Job ID = 6453301 SRX = SRX111779 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:25:56 prefetch.2.10.7: 1) Downloading 'SRR390223'... 2020-06-21T08:25:56 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:30:42 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:30:42 prefetch.2.10.7: 1) 'SRR390223' was downloaded successfully Read 20222375 spots for SRR390223/SRR390223.sra Written 20222375 spots for SRR390223/SRR390223.sra 2020-06-21T08:31:47 prefetch.2.10.7: 1) Downloading 'SRR390224'... 2020-06-21T08:31:47 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:37:02 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:37:02 prefetch.2.10.7: 1) 'SRR390224' was downloaded successfully Read 24290557 spots for SRR390224/SRR390224.sra Written 24290557 spots for SRR390224/SRR390224.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:18 44512932 reads; of these: 44512932 (100.00%) were unpaired; of these: 10737010 (24.12%) aligned 0 times 30040519 (67.49%) aligned exactly 1 time 3735403 (8.39%) aligned >1 times 75.88% overall alignment rate Time searching: 00:07:18 Overall time: 00:07:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 10546586 / 33775922 = 0.3123 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:53:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111779/SRX111779.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111779/SRX111779.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111779/SRX111779.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111779/SRX111779.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:53:05: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:53:05: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:53:11: 1000000 INFO @ Sun, 21 Jun 2020 17:53:17: 2000000 INFO @ Sun, 21 Jun 2020 17:53:23: 3000000 INFO @ Sun, 21 Jun 2020 17:53:30: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:53:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111779/SRX111779.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111779/SRX111779.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111779/SRX111779.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111779/SRX111779.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:53:35: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:53:35: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:53:36: 5000000 INFO @ Sun, 21 Jun 2020 17:53:42: 1000000 INFO @ Sun, 21 Jun 2020 17:53:43: 6000000 INFO @ Sun, 21 Jun 2020 17:53:48: 2000000 INFO @ Sun, 21 Jun 2020 17:53:49: 7000000 INFO @ Sun, 21 Jun 2020 17:53:55: 3000000 INFO @ Sun, 21 Jun 2020 17:53:56: 8000000 INFO @ Sun, 21 Jun 2020 17:54:02: 4000000 INFO @ Sun, 21 Jun 2020 17:54:02: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:54:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111779/SRX111779.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111779/SRX111779.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111779/SRX111779.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111779/SRX111779.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:54:05: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:54:05: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:54:09: 5000000 INFO @ Sun, 21 Jun 2020 17:54:09: 10000000 INFO @ Sun, 21 Jun 2020 17:54:11: 1000000 INFO @ Sun, 21 Jun 2020 17:54:15: 6000000 INFO @ Sun, 21 Jun 2020 17:54:16: 11000000 INFO @ Sun, 21 Jun 2020 17:54:18: 2000000 INFO @ Sun, 21 Jun 2020 17:54:22: 7000000 INFO @ Sun, 21 Jun 2020 17:54:23: 12000000 INFO @ Sun, 21 Jun 2020 17:54:24: 3000000 INFO @ Sun, 21 Jun 2020 17:54:29: 8000000 INFO @ Sun, 21 Jun 2020 17:54:30: 13000000 INFO @ Sun, 21 Jun 2020 17:54:31: 4000000 INFO @ Sun, 21 Jun 2020 17:54:36: 9000000 INFO @ Sun, 21 Jun 2020 17:54:36: 14000000 INFO @ Sun, 21 Jun 2020 17:54:38: 5000000 INFO @ Sun, 21 Jun 2020 17:54:43: 10000000 INFO @ Sun, 21 Jun 2020 17:54:43: 15000000 INFO @ Sun, 21 Jun 2020 17:54:44: 6000000 INFO @ Sun, 21 Jun 2020 17:54:50: 11000000 INFO @ Sun, 21 Jun 2020 17:54:50: 16000000 INFO @ Sun, 21 Jun 2020 17:54:51: 7000000 INFO @ Sun, 21 Jun 2020 17:54:56: 12000000 INFO @ Sun, 21 Jun 2020 17:54:57: 17000000 INFO @ Sun, 21 Jun 2020 17:54:57: 8000000 INFO @ Sun, 21 Jun 2020 17:55:03: 13000000 INFO @ Sun, 21 Jun 2020 17:55:04: 18000000 INFO @ Sun, 21 Jun 2020 17:55:04: 9000000 INFO @ Sun, 21 Jun 2020 17:55:10: 14000000 INFO @ Sun, 21 Jun 2020 17:55:10: 10000000 INFO @ Sun, 21 Jun 2020 17:55:10: 19000000 INFO @ Sun, 21 Jun 2020 17:55:17: 11000000 INFO @ Sun, 21 Jun 2020 17:55:17: 15000000 INFO @ Sun, 21 Jun 2020 17:55:18: 20000000 INFO @ Sun, 21 Jun 2020 17:55:23: 12000000 INFO @ Sun, 21 Jun 2020 17:55:24: 16000000 INFO @ Sun, 21 Jun 2020 17:55:24: 21000000 INFO @ Sun, 21 Jun 2020 17:55:30: 13000000 INFO @ Sun, 21 Jun 2020 17:55:31: 17000000 INFO @ Sun, 21 Jun 2020 17:55:31: 22000000 INFO @ Sun, 21 Jun 2020 17:55:37: 14000000 INFO @ Sun, 21 Jun 2020 17:55:38: 18000000 INFO @ Sun, 21 Jun 2020 17:55:38: 23000000 INFO @ Sun, 21 Jun 2020 17:55:40: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:55:40: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:55:40: #1 total tags in treatment: 23229336 INFO @ Sun, 21 Jun 2020 17:55:40: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:55:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:55:41: #1 tags after filtering in treatment: 23229335 INFO @ Sun, 21 Jun 2020 17:55:41: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:55:41: #1 finished! INFO @ Sun, 21 Jun 2020 17:55:41: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:55:41: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:55:42: #2 number of paired peaks: 75 WARNING @ Sun, 21 Jun 2020 17:55:42: Too few paired peaks (75) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:55:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111779/SRX111779.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111779/SRX111779.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111779/SRX111779.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111779/SRX111779.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:55:43: 15000000 INFO @ Sun, 21 Jun 2020 17:55:45: 19000000 INFO @ Sun, 21 Jun 2020 17:55:50: 16000000 INFO @ Sun, 21 Jun 2020 17:55:52: 20000000 INFO @ Sun, 21 Jun 2020 17:55:56: 17000000 INFO @ Sun, 21 Jun 2020 17:55:59: 21000000 INFO @ Sun, 21 Jun 2020 17:56:03: 18000000 INFO @ Sun, 21 Jun 2020 17:56:05: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:56:09: 19000000 INFO @ Sun, 21 Jun 2020 17:56:12: 23000000 INFO @ Sun, 21 Jun 2020 17:56:14: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:56:14: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:56:14: #1 total tags in treatment: 23229336 INFO @ Sun, 21 Jun 2020 17:56:14: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:56:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:56:15: #1 tags after filtering in treatment: 23229335 INFO @ Sun, 21 Jun 2020 17:56:15: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:56:15: #1 finished! INFO @ Sun, 21 Jun 2020 17:56:15: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:56:15: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:56:16: #2 number of paired peaks: 75 WARNING @ Sun, 21 Jun 2020 17:56:16: Too few paired peaks (75) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:56:16: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 17:56:16: 20000000 cut: /home/okishinya/chipatlas/results/dm6/SRX111779/SRX111779.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111779/SRX111779.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111779/SRX111779.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111779/SRX111779.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:56:22: 21000000 INFO @ Sun, 21 Jun 2020 17:56:29: 22000000 INFO @ Sun, 21 Jun 2020 17:56:35: 23000000 INFO @ Sun, 21 Jun 2020 17:56:36: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:56:36: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:56:36: #1 total tags in treatment: 23229336 INFO @ Sun, 21 Jun 2020 17:56:36: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:56:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:56:37: #1 tags after filtering in treatment: 23229335 INFO @ Sun, 21 Jun 2020 17:56:37: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:56:37: #1 finished! INFO @ Sun, 21 Jun 2020 17:56:37: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:56:37: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:56:38: #2 number of paired peaks: 75 WARNING @ Sun, 21 Jun 2020 17:56:38: Too few paired peaks (75) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:56:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111779/SRX111779.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111779/SRX111779.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111779/SRX111779.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111779/SRX111779.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。