Job ID = 6453275 SRX = SRX110787 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:13:33 prefetch.2.10.7: 1) Downloading 'SRR388369'... 2020-06-21T08:13:33 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:17:31 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:17:31 prefetch.2.10.7: 1) 'SRR388369' was downloaded successfully Read 39881305 spots for SRR388369/SRR388369.sra Written 39881305 spots for SRR388369/SRR388369.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:49 39881305 reads; of these: 39881305 (100.00%) were unpaired; of these: 1517403 (3.80%) aligned 0 times 29238938 (73.31%) aligned exactly 1 time 9124964 (22.88%) aligned >1 times 96.20% overall alignment rate Time searching: 00:08:49 Overall time: 00:08:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5376953 / 38363902 = 0.1402 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:36:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX110787/SRX110787.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX110787/SRX110787.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX110787/SRX110787.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX110787/SRX110787.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:36:30: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:36:30: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:36:36: 1000000 INFO @ Sun, 21 Jun 2020 17:36:41: 2000000 INFO @ Sun, 21 Jun 2020 17:36:47: 3000000 INFO @ Sun, 21 Jun 2020 17:36:52: 4000000 INFO @ Sun, 21 Jun 2020 17:36:58: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:37:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX110787/SRX110787.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX110787/SRX110787.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX110787/SRX110787.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX110787/SRX110787.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:37:00: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:37:00: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:37:04: 6000000 INFO @ Sun, 21 Jun 2020 17:37:07: 1000000 INFO @ Sun, 21 Jun 2020 17:37:10: 7000000 INFO @ Sun, 21 Jun 2020 17:37:15: 2000000 INFO @ Sun, 21 Jun 2020 17:37:17: 8000000 INFO @ Sun, 21 Jun 2020 17:37:21: 3000000 INFO @ Sun, 21 Jun 2020 17:37:23: 9000000 BedGraph に変換中... INFO @ Sun, 21 Jun 2020 17:37:28: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:37:29: 10000000 INFO @ Sun, 21 Jun 2020 17:37:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX110787/SRX110787.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX110787/SRX110787.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX110787/SRX110787.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX110787/SRX110787.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:37:30: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:37:30: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:37:35: 5000000 INFO @ Sun, 21 Jun 2020 17:37:36: 11000000 INFO @ Sun, 21 Jun 2020 17:37:37: 1000000 INFO @ Sun, 21 Jun 2020 17:37:42: 12000000 INFO @ Sun, 21 Jun 2020 17:37:42: 6000000 INFO @ Sun, 21 Jun 2020 17:37:43: 2000000 INFO @ Sun, 21 Jun 2020 17:37:49: 13000000 INFO @ Sun, 21 Jun 2020 17:37:49: 7000000 INFO @ Sun, 21 Jun 2020 17:37:50: 3000000 INFO @ Sun, 21 Jun 2020 17:37:55: 14000000 INFO @ Sun, 21 Jun 2020 17:37:56: 8000000 INFO @ Sun, 21 Jun 2020 17:37:56: 4000000 INFO @ Sun, 21 Jun 2020 17:38:02: 15000000 INFO @ Sun, 21 Jun 2020 17:38:03: 9000000 INFO @ Sun, 21 Jun 2020 17:38:03: 5000000 INFO @ Sun, 21 Jun 2020 17:38:08: 16000000 INFO @ Sun, 21 Jun 2020 17:38:09: 10000000 INFO @ Sun, 21 Jun 2020 17:38:09: 6000000 INFO @ Sun, 21 Jun 2020 17:38:14: 17000000 INFO @ Sun, 21 Jun 2020 17:38:16: 7000000 INFO @ Sun, 21 Jun 2020 17:38:16: 11000000 INFO @ Sun, 21 Jun 2020 17:38:21: 18000000 INFO @ Sun, 21 Jun 2020 17:38:22: 12000000 INFO @ Sun, 21 Jun 2020 17:38:22: 8000000 INFO @ Sun, 21 Jun 2020 17:38:27: 19000000 INFO @ Sun, 21 Jun 2020 17:38:29: 13000000 INFO @ Sun, 21 Jun 2020 17:38:29: 9000000 INFO @ Sun, 21 Jun 2020 17:38:33: 20000000 INFO @ Sun, 21 Jun 2020 17:38:35: 14000000 INFO @ Sun, 21 Jun 2020 17:38:35: 10000000 INFO @ Sun, 21 Jun 2020 17:38:40: 21000000 INFO @ Sun, 21 Jun 2020 17:38:41: 15000000 INFO @ Sun, 21 Jun 2020 17:38:42: 11000000 INFO @ Sun, 21 Jun 2020 17:38:46: 22000000 INFO @ Sun, 21 Jun 2020 17:38:48: 16000000 INFO @ Sun, 21 Jun 2020 17:38:48: 12000000 INFO @ Sun, 21 Jun 2020 17:38:52: 23000000 INFO @ Sun, 21 Jun 2020 17:38:54: 17000000 INFO @ Sun, 21 Jun 2020 17:38:55: 13000000 INFO @ Sun, 21 Jun 2020 17:38:59: 24000000 INFO @ Sun, 21 Jun 2020 17:39:01: 18000000 INFO @ Sun, 21 Jun 2020 17:39:01: 14000000 INFO @ Sun, 21 Jun 2020 17:39:05: 25000000 INFO @ Sun, 21 Jun 2020 17:39:07: 19000000 INFO @ Sun, 21 Jun 2020 17:39:07: 15000000 INFO @ Sun, 21 Jun 2020 17:39:11: 26000000 INFO @ Sun, 21 Jun 2020 17:39:13: 20000000 INFO @ Sun, 21 Jun 2020 17:39:14: 16000000 INFO @ Sun, 21 Jun 2020 17:39:18: 27000000 INFO @ Sun, 21 Jun 2020 17:39:20: 21000000 INFO @ Sun, 21 Jun 2020 17:39:20: 17000000 INFO @ Sun, 21 Jun 2020 17:39:24: 28000000 INFO @ Sun, 21 Jun 2020 17:39:26: 22000000 INFO @ Sun, 21 Jun 2020 17:39:26: 18000000 INFO @ Sun, 21 Jun 2020 17:39:31: 29000000 INFO @ Sun, 21 Jun 2020 17:39:32: 23000000 INFO @ Sun, 21 Jun 2020 17:39:33: 19000000 INFO @ Sun, 21 Jun 2020 17:39:37: 30000000 INFO @ Sun, 21 Jun 2020 17:39:39: 24000000 INFO @ Sun, 21 Jun 2020 17:39:39: 20000000 INFO @ Sun, 21 Jun 2020 17:39:43: 31000000 INFO @ Sun, 21 Jun 2020 17:39:45: 25000000 INFO @ Sun, 21 Jun 2020 17:39:45: 21000000 INFO @ Sun, 21 Jun 2020 17:39:50: 32000000 INFO @ Sun, 21 Jun 2020 17:39:51: 26000000 INFO @ Sun, 21 Jun 2020 17:39:52: 22000000 INFO @ Sun, 21 Jun 2020 17:39:57: #1 tag size is determined as 40 bps INFO @ Sun, 21 Jun 2020 17:39:57: #1 tag size = 40 INFO @ Sun, 21 Jun 2020 17:39:57: #1 total tags in treatment: 32986949 INFO @ Sun, 21 Jun 2020 17:39:57: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:39:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:39:57: #1 tags after filtering in treatment: 32986949 INFO @ Sun, 21 Jun 2020 17:39:57: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:39:57: #1 finished! INFO @ Sun, 21 Jun 2020 17:39:57: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:39:57: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:39:58: 27000000 INFO @ Sun, 21 Jun 2020 17:39:58: 23000000 INFO @ Sun, 21 Jun 2020 17:39:59: #2 number of paired peaks: 70 WARNING @ Sun, 21 Jun 2020 17:39:59: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:39:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX110787/SRX110787.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX110787/SRX110787.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX110787/SRX110787.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX110787/SRX110787.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:40:04: 28000000 INFO @ Sun, 21 Jun 2020 17:40:05: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:40:11: 29000000 INFO @ Sun, 21 Jun 2020 17:40:11: 25000000 INFO @ Sun, 21 Jun 2020 17:40:17: 30000000 INFO @ Sun, 21 Jun 2020 17:40:17: 26000000 INFO @ Sun, 21 Jun 2020 17:40:24: 31000000 INFO @ Sun, 21 Jun 2020 17:40:24: 27000000 INFO @ Sun, 21 Jun 2020 17:40:30: 32000000 INFO @ Sun, 21 Jun 2020 17:40:31: 28000000 INFO @ Sun, 21 Jun 2020 17:40:37: #1 tag size is determined as 40 bps INFO @ Sun, 21 Jun 2020 17:40:37: #1 tag size = 40 INFO @ Sun, 21 Jun 2020 17:40:37: #1 total tags in treatment: 32986949 INFO @ Sun, 21 Jun 2020 17:40:37: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:40:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:40:37: 29000000 INFO @ Sun, 21 Jun 2020 17:40:38: #1 tags after filtering in treatment: 32986949 INFO @ Sun, 21 Jun 2020 17:40:38: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:40:38: #1 finished! INFO @ Sun, 21 Jun 2020 17:40:38: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:40:38: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:40:40: #2 number of paired peaks: 70 WARNING @ Sun, 21 Jun 2020 17:40:40: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:40:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX110787/SRX110787.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX110787/SRX110787.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX110787/SRX110787.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX110787/SRX110787.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:40:43: 30000000 INFO @ Sun, 21 Jun 2020 17:40:49: 31000000 INFO @ Sun, 21 Jun 2020 17:40:55: 32000000 INFO @ Sun, 21 Jun 2020 17:41:01: #1 tag size is determined as 40 bps INFO @ Sun, 21 Jun 2020 17:41:01: #1 tag size = 40 INFO @ Sun, 21 Jun 2020 17:41:01: #1 total tags in treatment: 32986949 INFO @ Sun, 21 Jun 2020 17:41:01: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:41:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:41:02: #1 tags after filtering in treatment: 32986949 INFO @ Sun, 21 Jun 2020 17:41:02: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:41:02: #1 finished! INFO @ Sun, 21 Jun 2020 17:41:02: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:41:02: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:41:04: #2 number of paired peaks: 70 WARNING @ Sun, 21 Jun 2020 17:41:04: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:41:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX110787/SRX110787.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX110787/SRX110787.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX110787/SRX110787.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX110787/SRX110787.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。