Job ID = 6453269 SRX = SRX110783 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:25:02 prefetch.2.10.7: 1) Downloading 'SRR388365'... 2020-06-21T08:25:02 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:30:29 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:30:29 prefetch.2.10.7: 1) 'SRR388365' was downloaded successfully Read 35029531 spots for SRR388365/SRR388365.sra Written 35029531 spots for SRR388365/SRR388365.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:47 35029531 reads; of these: 35029531 (100.00%) were unpaired; of these: 15397466 (43.96%) aligned 0 times 18050572 (51.53%) aligned exactly 1 time 1581493 (4.51%) aligned >1 times 56.04% overall alignment rate Time searching: 00:04:48 Overall time: 00:04:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 8935140 / 19632065 = 0.4551 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:40:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX110783/SRX110783.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX110783/SRX110783.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX110783/SRX110783.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX110783/SRX110783.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:40:39: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:40:39: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:40:45: 1000000 INFO @ Sun, 21 Jun 2020 17:40:50: 2000000 INFO @ Sun, 21 Jun 2020 17:40:56: 3000000 INFO @ Sun, 21 Jun 2020 17:41:01: 4000000 INFO @ Sun, 21 Jun 2020 17:41:06: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:41:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX110783/SRX110783.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX110783/SRX110783.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX110783/SRX110783.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX110783/SRX110783.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:41:09: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:41:09: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:41:12: 6000000 INFO @ Sun, 21 Jun 2020 17:41:16: 1000000 INFO @ Sun, 21 Jun 2020 17:41:19: 7000000 INFO @ Sun, 21 Jun 2020 17:41:24: 2000000 INFO @ Sun, 21 Jun 2020 17:41:26: 8000000 INFO @ Sun, 21 Jun 2020 17:41:31: 3000000 INFO @ Sun, 21 Jun 2020 17:41:33: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:41:38: 4000000 INFO @ Sun, 21 Jun 2020 17:41:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX110783/SRX110783.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX110783/SRX110783.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX110783/SRX110783.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX110783/SRX110783.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:41:39: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:41:39: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:41:39: 10000000 INFO @ Sun, 21 Jun 2020 17:41:44: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 17:41:44: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 17:41:44: #1 total tags in treatment: 10696925 INFO @ Sun, 21 Jun 2020 17:41:44: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:41:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:41:45: #1 tags after filtering in treatment: 10696906 INFO @ Sun, 21 Jun 2020 17:41:45: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:41:45: #1 finished! INFO @ Sun, 21 Jun 2020 17:41:45: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:41:45: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:41:45: 5000000 INFO @ Sun, 21 Jun 2020 17:41:46: #2 number of paired peaks: 7651 INFO @ Sun, 21 Jun 2020 17:41:46: start model_add_line... INFO @ Sun, 21 Jun 2020 17:41:46: 1000000 INFO @ Sun, 21 Jun 2020 17:41:46: start X-correlation... INFO @ Sun, 21 Jun 2020 17:41:46: end of X-cor INFO @ Sun, 21 Jun 2020 17:41:46: #2 finished! INFO @ Sun, 21 Jun 2020 17:41:46: #2 predicted fragment length is 95 bps INFO @ Sun, 21 Jun 2020 17:41:46: #2 alternative fragment length(s) may be 95 bps INFO @ Sun, 21 Jun 2020 17:41:46: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX110783/SRX110783.05_model.r INFO @ Sun, 21 Jun 2020 17:41:46: #3 Call peaks... INFO @ Sun, 21 Jun 2020 17:41:46: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 21 Jun 2020 17:41:51: 6000000 INFO @ Sun, 21 Jun 2020 17:41:52: 2000000 INFO @ Sun, 21 Jun 2020 17:41:57: 7000000 INFO @ Sun, 21 Jun 2020 17:41:59: 3000000 INFO @ Sun, 21 Jun 2020 17:42:04: 8000000 INFO @ Sun, 21 Jun 2020 17:42:05: 4000000 INFO @ Sun, 21 Jun 2020 17:42:11: 9000000 INFO @ Sun, 21 Jun 2020 17:42:11: #3 Call peaks for each chromosome... INFO @ Sun, 21 Jun 2020 17:42:12: 5000000 INFO @ Sun, 21 Jun 2020 17:42:18: 10000000 INFO @ Sun, 21 Jun 2020 17:42:18: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:42:23: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 17:42:23: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 17:42:23: #1 total tags in treatment: 10696925 INFO @ Sun, 21 Jun 2020 17:42:23: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:42:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:42:23: #1 tags after filtering in treatment: 10696906 INFO @ Sun, 21 Jun 2020 17:42:23: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:42:23: #1 finished! INFO @ Sun, 21 Jun 2020 17:42:23: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:42:23: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:42:23: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX110783/SRX110783.05_peaks.xls INFO @ Sun, 21 Jun 2020 17:42:24: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX110783/SRX110783.05_peaks.narrowPeak INFO @ Sun, 21 Jun 2020 17:42:24: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX110783/SRX110783.05_summits.bed INFO @ Sun, 21 Jun 2020 17:42:24: Done! pass1 - making usageList (111 chroms): 2 millis pass2 - checking and writing primary data (7305 records, 4 fields): 10 millis CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:42:25: #2 number of paired peaks: 7651 INFO @ Sun, 21 Jun 2020 17:42:25: start model_add_line... INFO @ Sun, 21 Jun 2020 17:42:25: 7000000 INFO @ Sun, 21 Jun 2020 17:42:25: start X-correlation... INFO @ Sun, 21 Jun 2020 17:42:25: end of X-cor INFO @ Sun, 21 Jun 2020 17:42:25: #2 finished! INFO @ Sun, 21 Jun 2020 17:42:25: #2 predicted fragment length is 95 bps INFO @ Sun, 21 Jun 2020 17:42:25: #2 alternative fragment length(s) may be 95 bps INFO @ Sun, 21 Jun 2020 17:42:25: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX110783/SRX110783.10_model.r INFO @ Sun, 21 Jun 2020 17:42:25: #3 Call peaks... INFO @ Sun, 21 Jun 2020 17:42:25: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 21 Jun 2020 17:42:31: 8000000 INFO @ Sun, 21 Jun 2020 17:42:37: 9000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 17:42:42: 10000000 INFO @ Sun, 21 Jun 2020 17:42:46: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 17:42:46: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 17:42:46: #1 total tags in treatment: 10696925 INFO @ Sun, 21 Jun 2020 17:42:46: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:42:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:42:47: #1 tags after filtering in treatment: 10696906 INFO @ Sun, 21 Jun 2020 17:42:47: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:42:47: #1 finished! INFO @ Sun, 21 Jun 2020 17:42:47: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:42:47: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:42:48: #2 number of paired peaks: 7651 INFO @ Sun, 21 Jun 2020 17:42:48: start model_add_line... INFO @ Sun, 21 Jun 2020 17:42:48: start X-correlation... INFO @ Sun, 21 Jun 2020 17:42:48: end of X-cor INFO @ Sun, 21 Jun 2020 17:42:48: #2 finished! INFO @ Sun, 21 Jun 2020 17:42:48: #2 predicted fragment length is 95 bps INFO @ Sun, 21 Jun 2020 17:42:48: #2 alternative fragment length(s) may be 95 bps INFO @ Sun, 21 Jun 2020 17:42:48: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX110783/SRX110783.20_model.r INFO @ Sun, 21 Jun 2020 17:42:48: #3 Call peaks... INFO @ Sun, 21 Jun 2020 17:42:48: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 21 Jun 2020 17:42:50: #3 Call peaks for each chromosome... INFO @ Sun, 21 Jun 2020 17:43:03: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX110783/SRX110783.10_peaks.xls INFO @ Sun, 21 Jun 2020 17:43:03: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX110783/SRX110783.10_peaks.narrowPeak INFO @ Sun, 21 Jun 2020 17:43:04: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX110783/SRX110783.10_summits.bed INFO @ Sun, 21 Jun 2020 17:43:04: Done! pass1 - making usageList (88 chroms): 1 millis pass2 - checking and writing primary data (6541 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:43:13: #3 Call peaks for each chromosome... INFO @ Sun, 21 Jun 2020 17:43:26: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX110783/SRX110783.20_peaks.xls INFO @ Sun, 21 Jun 2020 17:43:26: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX110783/SRX110783.20_peaks.narrowPeak INFO @ Sun, 21 Jun 2020 17:43:26: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX110783/SRX110783.20_summits.bed INFO @ Sun, 21 Jun 2020 17:43:26: Done! pass1 - making usageList (65 chroms): 1 millis pass2 - checking and writing primary data (5679 records, 4 fields): 9 millis CompletedMACS2peakCalling