Job ID = 6453231 SRX = SRX110510 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:12:48 prefetch.2.10.7: 1) Downloading 'SRR387831'... 2020-06-21T08:12:48 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:16:27 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:16:27 prefetch.2.10.7: 1) 'SRR387831' was downloaded successfully Read 19141145 spots for SRR387831/SRR387831.sra Written 19141145 spots for SRR387831/SRR387831.sra 2020-06-21T08:17:35 prefetch.2.10.7: 1) Downloading 'SRR387832'... 2020-06-21T08:17:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:20:57 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:20:57 prefetch.2.10.7: 1) 'SRR387832' was downloaded successfully Read 23287832 spots for SRR387832/SRR387832.sra Written 23287832 spots for SRR387832/SRR387832.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:14 42428977 reads; of these: 42428977 (100.00%) were unpaired; of these: 5192995 (12.24%) aligned 0 times 32974031 (77.72%) aligned exactly 1 time 4261951 (10.04%) aligned >1 times 87.76% overall alignment rate Time searching: 00:08:14 Overall time: 00:08:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 11188614 / 37235982 = 0.3005 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:38:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX110510/SRX110510.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX110510/SRX110510.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX110510/SRX110510.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX110510/SRX110510.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:38:24: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:38:24: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:38:30: 1000000 INFO @ Sun, 21 Jun 2020 17:38:35: 2000000 INFO @ Sun, 21 Jun 2020 17:38:40: 3000000 INFO @ Sun, 21 Jun 2020 17:38:46: 4000000 INFO @ Sun, 21 Jun 2020 17:38:51: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:38:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX110510/SRX110510.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX110510/SRX110510.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX110510/SRX110510.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX110510/SRX110510.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:38:54: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:38:54: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:38:57: 6000000 INFO @ Sun, 21 Jun 2020 17:39:00: 1000000 INFO @ Sun, 21 Jun 2020 17:39:03: 7000000 INFO @ Sun, 21 Jun 2020 17:39:06: 2000000 INFO @ Sun, 21 Jun 2020 17:39:09: 8000000 INFO @ Sun, 21 Jun 2020 17:39:12: 3000000 INFO @ Sun, 21 Jun 2020 17:39:15: 9000000 INFO @ Sun, 21 Jun 2020 17:39:18: 4000000 INFO @ Sun, 21 Jun 2020 17:39:21: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:39:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX110510/SRX110510.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX110510/SRX110510.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX110510/SRX110510.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX110510/SRX110510.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:39:24: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:39:24: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:39:24: 5000000 INFO @ Sun, 21 Jun 2020 17:39:27: 11000000 INFO @ Sun, 21 Jun 2020 17:39:31: 6000000 INFO @ Sun, 21 Jun 2020 17:39:31: 1000000 INFO @ Sun, 21 Jun 2020 17:39:34: 12000000 INFO @ Sun, 21 Jun 2020 17:39:37: 7000000 INFO @ Sun, 21 Jun 2020 17:39:39: 2000000 INFO @ Sun, 21 Jun 2020 17:39:40: 13000000 INFO @ Sun, 21 Jun 2020 17:39:44: 8000000 INFO @ Sun, 21 Jun 2020 17:39:46: 3000000 INFO @ Sun, 21 Jun 2020 17:39:47: 14000000 INFO @ Sun, 21 Jun 2020 17:39:50: 9000000 INFO @ Sun, 21 Jun 2020 17:39:53: 4000000 INFO @ Sun, 21 Jun 2020 17:39:53: 15000000 INFO @ Sun, 21 Jun 2020 17:39:57: 10000000 INFO @ Sun, 21 Jun 2020 17:40:00: 16000000 INFO @ Sun, 21 Jun 2020 17:40:00: 5000000 INFO @ Sun, 21 Jun 2020 17:40:03: 11000000 INFO @ Sun, 21 Jun 2020 17:40:06: 17000000 INFO @ Sun, 21 Jun 2020 17:40:07: 6000000 INFO @ Sun, 21 Jun 2020 17:40:10: 12000000 INFO @ Sun, 21 Jun 2020 17:40:12: 18000000 INFO @ Sun, 21 Jun 2020 17:40:14: 7000000 INFO @ Sun, 21 Jun 2020 17:40:16: 13000000 INFO @ Sun, 21 Jun 2020 17:40:19: 19000000 INFO @ Sun, 21 Jun 2020 17:40:21: 8000000 INFO @ Sun, 21 Jun 2020 17:40:23: 14000000 INFO @ Sun, 21 Jun 2020 17:40:25: 20000000 INFO @ Sun, 21 Jun 2020 17:40:28: 9000000 INFO @ Sun, 21 Jun 2020 17:40:29: 15000000 INFO @ Sun, 21 Jun 2020 17:40:32: 21000000 INFO @ Sun, 21 Jun 2020 17:40:35: 16000000 INFO @ Sun, 21 Jun 2020 17:40:36: 10000000 INFO @ Sun, 21 Jun 2020 17:40:38: 22000000 INFO @ Sun, 21 Jun 2020 17:40:42: 17000000 INFO @ Sun, 21 Jun 2020 17:40:43: 11000000 INFO @ Sun, 21 Jun 2020 17:40:45: 23000000 INFO @ Sun, 21 Jun 2020 17:40:48: 18000000 INFO @ Sun, 21 Jun 2020 17:40:50: 12000000 INFO @ Sun, 21 Jun 2020 17:40:51: 24000000 INFO @ Sun, 21 Jun 2020 17:40:55: 19000000 INFO @ Sun, 21 Jun 2020 17:40:57: 13000000 INFO @ Sun, 21 Jun 2020 17:40:58: 25000000 INFO @ Sun, 21 Jun 2020 17:41:01: 20000000 INFO @ Sun, 21 Jun 2020 17:41:04: 14000000 INFO @ Sun, 21 Jun 2020 17:41:04: 26000000 INFO @ Sun, 21 Jun 2020 17:41:05: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:41:05: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:41:05: #1 total tags in treatment: 26047368 INFO @ Sun, 21 Jun 2020 17:41:05: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:41:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:41:05: #1 tags after filtering in treatment: 26047336 INFO @ Sun, 21 Jun 2020 17:41:05: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:41:05: #1 finished! INFO @ Sun, 21 Jun 2020 17:41:05: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:41:05: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:41:07: #2 number of paired peaks: 1 WARNING @ Sun, 21 Jun 2020 17:41:07: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:41:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX110510/SRX110510.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX110510/SRX110510.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX110510/SRX110510.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX110510/SRX110510.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:41:08: 21000000 INFO @ Sun, 21 Jun 2020 17:41:11: 15000000 INFO @ Sun, 21 Jun 2020 17:41:14: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:41:18: 16000000 INFO @ Sun, 21 Jun 2020 17:41:20: 23000000 INFO @ Sun, 21 Jun 2020 17:41:25: 17000000 INFO @ Sun, 21 Jun 2020 17:41:27: 24000000 INFO @ Sun, 21 Jun 2020 17:41:32: 18000000 INFO @ Sun, 21 Jun 2020 17:41:33: 25000000 INFO @ Sun, 21 Jun 2020 17:41:38: 19000000 INFO @ Sun, 21 Jun 2020 17:41:39: 26000000 INFO @ Sun, 21 Jun 2020 17:41:40: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:41:40: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:41:40: #1 total tags in treatment: 26047368 INFO @ Sun, 21 Jun 2020 17:41:40: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:41:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:41:41: #1 tags after filtering in treatment: 26047336 INFO @ Sun, 21 Jun 2020 17:41:41: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:41:41: #1 finished! INFO @ Sun, 21 Jun 2020 17:41:41: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:41:41: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:41:42: #2 number of paired peaks: 1 WARNING @ Sun, 21 Jun 2020 17:41:42: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:41:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX110510/SRX110510.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX110510/SRX110510.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX110510/SRX110510.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX110510/SRX110510.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:41:45: 20000000 INFO @ Sun, 21 Jun 2020 17:41:52: 21000000 INFO @ Sun, 21 Jun 2020 17:41:58: 22000000 INFO @ Sun, 21 Jun 2020 17:42:04: 23000000 INFO @ Sun, 21 Jun 2020 17:42:11: 24000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 17:42:17: 25000000 INFO @ Sun, 21 Jun 2020 17:42:23: 26000000 INFO @ Sun, 21 Jun 2020 17:42:24: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:42:24: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:42:24: #1 total tags in treatment: 26047368 INFO @ Sun, 21 Jun 2020 17:42:24: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:42:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:42:24: #1 tags after filtering in treatment: 26047336 INFO @ Sun, 21 Jun 2020 17:42:24: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:42:24: #1 finished! INFO @ Sun, 21 Jun 2020 17:42:24: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:42:24: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:42:26: #2 number of paired peaks: 1 WARNING @ Sun, 21 Jun 2020 17:42:26: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:42:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX110510/SRX110510.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX110510/SRX110510.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX110510/SRX110510.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX110510/SRX110510.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling