Job ID = 14167741 SRX = SRX10760659 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:53 16428574 reads; of these: 16428574 (100.00%) were unpaired; of these: 4566327 (27.80%) aligned 0 times 10668929 (64.94%) aligned exactly 1 time 1193318 (7.26%) aligned >1 times 72.20% overall alignment rate Time searching: 00:03:53 Overall time: 00:03:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2976834 / 11862247 = 0.2510 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:22:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX10760659/SRX10760659.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX10760659/SRX10760659.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX10760659/SRX10760659.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX10760659/SRX10760659.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:22:14: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:22:14: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:22:22: 1000000 INFO @ Fri, 10 Dec 2021 13:22:30: 2000000 INFO @ Fri, 10 Dec 2021 13:22:38: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:22:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX10760659/SRX10760659.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX10760659/SRX10760659.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX10760659/SRX10760659.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX10760659/SRX10760659.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:22:44: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:22:44: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:22:46: 4000000 INFO @ Fri, 10 Dec 2021 13:22:52: 1000000 INFO @ Fri, 10 Dec 2021 13:22:55: 5000000 INFO @ Fri, 10 Dec 2021 13:23:01: 2000000 INFO @ Fri, 10 Dec 2021 13:23:04: 6000000 INFO @ Fri, 10 Dec 2021 13:23:09: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:23:13: 7000000 INFO @ Fri, 10 Dec 2021 13:23:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX10760659/SRX10760659.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX10760659/SRX10760659.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX10760659/SRX10760659.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX10760659/SRX10760659.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:23:14: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:23:14: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:23:17: 4000000 INFO @ Fri, 10 Dec 2021 13:23:22: 8000000 INFO @ Fri, 10 Dec 2021 13:23:24: 1000000 INFO @ Fri, 10 Dec 2021 13:23:25: 5000000 INFO @ Fri, 10 Dec 2021 13:23:30: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 13:23:30: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 13:23:30: #1 total tags in treatment: 8885413 INFO @ Fri, 10 Dec 2021 13:23:30: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:23:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:23:30: #1 tags after filtering in treatment: 8884901 INFO @ Fri, 10 Dec 2021 13:23:30: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:23:30: #1 finished! INFO @ Fri, 10 Dec 2021 13:23:30: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:23:30: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:23:32: #2 number of paired peaks: 7277 INFO @ Fri, 10 Dec 2021 13:23:32: start model_add_line... INFO @ Fri, 10 Dec 2021 13:23:32: start X-correlation... INFO @ Fri, 10 Dec 2021 13:23:32: end of X-cor INFO @ Fri, 10 Dec 2021 13:23:32: #2 finished! INFO @ Fri, 10 Dec 2021 13:23:32: #2 predicted fragment length is 181 bps INFO @ Fri, 10 Dec 2021 13:23:32: #2 alternative fragment length(s) may be 181 bps INFO @ Fri, 10 Dec 2021 13:23:32: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX10760659/SRX10760659.05_model.r INFO @ Fri, 10 Dec 2021 13:23:32: #3 Call peaks... INFO @ Fri, 10 Dec 2021 13:23:32: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 13:23:32: 2000000 INFO @ Fri, 10 Dec 2021 13:23:33: 6000000 INFO @ Fri, 10 Dec 2021 13:23:41: 3000000 INFO @ Fri, 10 Dec 2021 13:23:41: 7000000 INFO @ Fri, 10 Dec 2021 13:23:50: 8000000 INFO @ Fri, 10 Dec 2021 13:23:50: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 13:23:57: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 13:23:57: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 13:23:57: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 13:23:57: #1 total tags in treatment: 8885413 INFO @ Fri, 10 Dec 2021 13:23:57: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:23:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:23:57: #1 tags after filtering in treatment: 8884901 INFO @ Fri, 10 Dec 2021 13:23:57: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:23:57: #1 finished! INFO @ Fri, 10 Dec 2021 13:23:57: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:23:57: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:23:58: #2 number of paired peaks: 7277 INFO @ Fri, 10 Dec 2021 13:23:58: start model_add_line... INFO @ Fri, 10 Dec 2021 13:23:58: start X-correlation... INFO @ Fri, 10 Dec 2021 13:23:58: end of X-cor INFO @ Fri, 10 Dec 2021 13:23:58: #2 finished! INFO @ Fri, 10 Dec 2021 13:23:58: #2 predicted fragment length is 181 bps INFO @ Fri, 10 Dec 2021 13:23:58: #2 alternative fragment length(s) may be 181 bps INFO @ Fri, 10 Dec 2021 13:23:58: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX10760659/SRX10760659.10_model.r INFO @ Fri, 10 Dec 2021 13:23:59: #3 Call peaks... INFO @ Fri, 10 Dec 2021 13:23:59: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 13:23:59: 5000000 INFO @ Fri, 10 Dec 2021 13:24:06: 6000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 13:24:09: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX10760659/SRX10760659.05_peaks.xls INFO @ Fri, 10 Dec 2021 13:24:09: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX10760659/SRX10760659.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 13:24:09: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX10760659/SRX10760659.05_summits.bed INFO @ Fri, 10 Dec 2021 13:24:09: Done! pass1 - making usageList (182 chroms): 2 millis pass2 - checking and writing primary data (13945 records, 4 fields): 20 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 13:24:14: 7000000 INFO @ Fri, 10 Dec 2021 13:24:22: 8000000 INFO @ Fri, 10 Dec 2021 13:24:24: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 13:24:29: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 13:24:29: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 13:24:29: #1 total tags in treatment: 8885413 INFO @ Fri, 10 Dec 2021 13:24:29: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:24:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:24:29: #1 tags after filtering in treatment: 8884901 INFO @ Fri, 10 Dec 2021 13:24:29: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:24:29: #1 finished! INFO @ Fri, 10 Dec 2021 13:24:29: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:24:29: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:24:30: #2 number of paired peaks: 7277 INFO @ Fri, 10 Dec 2021 13:24:30: start model_add_line... INFO @ Fri, 10 Dec 2021 13:24:30: start X-correlation... INFO @ Fri, 10 Dec 2021 13:24:30: end of X-cor INFO @ Fri, 10 Dec 2021 13:24:30: #2 finished! INFO @ Fri, 10 Dec 2021 13:24:30: #2 predicted fragment length is 181 bps INFO @ Fri, 10 Dec 2021 13:24:30: #2 alternative fragment length(s) may be 181 bps INFO @ Fri, 10 Dec 2021 13:24:30: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX10760659/SRX10760659.20_model.r INFO @ Fri, 10 Dec 2021 13:24:30: #3 Call peaks... INFO @ Fri, 10 Dec 2021 13:24:30: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 13:24:35: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX10760659/SRX10760659.10_peaks.xls INFO @ Fri, 10 Dec 2021 13:24:35: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX10760659/SRX10760659.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 13:24:35: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX10760659/SRX10760659.10_summits.bed INFO @ Fri, 10 Dec 2021 13:24:35: Done! pass1 - making usageList (135 chroms): 3 millis pass2 - checking and writing primary data (9700 records, 4 fields): 15 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 13:24:55: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 13:25:06: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX10760659/SRX10760659.20_peaks.xls INFO @ Fri, 10 Dec 2021 13:25:06: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX10760659/SRX10760659.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 13:25:06: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX10760659/SRX10760659.20_summits.bed INFO @ Fri, 10 Dec 2021 13:25:06: Done! pass1 - making usageList (91 chroms): 1 millis pass2 - checking and writing primary data (6347 records, 4 fields): 12 millis CompletedMACS2peakCalling