Job ID = 6453123 SRX = SRX1038029 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:17:19 prefetch.2.10.7: 1) Downloading 'SRR2039658'... 2020-06-21T08:17:19 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:19:47 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:19:48 prefetch.2.10.7: 'SRR2039658' is valid 2020-06-21T08:19:48 prefetch.2.10.7: 1) 'SRR2039658' was downloaded successfully Read 9781054 spots for SRR2039658/SRR2039658.sra Written 9781054 spots for SRR2039658/SRR2039658.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:07 9781054 reads; of these: 9781054 (100.00%) were unpaired; of these: 7572914 (77.42%) aligned 0 times 2016712 (20.62%) aligned exactly 1 time 191428 (1.96%) aligned >1 times 22.58% overall alignment rate Time searching: 00:01:07 Overall time: 00:01:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 422039 / 2208140 = 0.1911 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:22:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1038029/SRX1038029.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1038029/SRX1038029.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1038029/SRX1038029.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1038029/SRX1038029.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:22:33: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:22:33: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:22:39: 1000000 INFO @ Sun, 21 Jun 2020 17:22:44: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 17:22:44: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 17:22:44: #1 total tags in treatment: 1786101 INFO @ Sun, 21 Jun 2020 17:22:44: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:22:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:22:44: #1 tags after filtering in treatment: 1785494 INFO @ Sun, 21 Jun 2020 17:22:44: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:22:44: #1 finished! INFO @ Sun, 21 Jun 2020 17:22:44: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:22:44: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:22:44: #2 number of paired peaks: 4991 INFO @ Sun, 21 Jun 2020 17:22:44: start model_add_line... INFO @ Sun, 21 Jun 2020 17:22:44: start X-correlation... INFO @ Sun, 21 Jun 2020 17:22:44: end of X-cor INFO @ Sun, 21 Jun 2020 17:22:44: #2 finished! INFO @ Sun, 21 Jun 2020 17:22:44: #2 predicted fragment length is 153 bps INFO @ Sun, 21 Jun 2020 17:22:44: #2 alternative fragment length(s) may be 153 bps INFO @ Sun, 21 Jun 2020 17:22:44: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX1038029/SRX1038029.05_model.r INFO @ Sun, 21 Jun 2020 17:22:44: #3 Call peaks... INFO @ Sun, 21 Jun 2020 17:22:44: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 21 Jun 2020 17:22:49: #3 Call peaks for each chromosome... INFO @ Sun, 21 Jun 2020 17:22:52: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX1038029/SRX1038029.05_peaks.xls INFO @ Sun, 21 Jun 2020 17:22:52: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX1038029/SRX1038029.05_peaks.narrowPeak INFO @ Sun, 21 Jun 2020 17:22:52: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX1038029/SRX1038029.05_summits.bed INFO @ Sun, 21 Jun 2020 17:22:52: Done! pass1 - making usageList (90 chroms): 1 millis pass2 - checking and writing primary data (1995 records, 4 fields): 6 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:23:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1038029/SRX1038029.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1038029/SRX1038029.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1038029/SRX1038029.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1038029/SRX1038029.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:23:04: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:23:04: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:23:11: 1000000 INFO @ Sun, 21 Jun 2020 17:23:16: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 17:23:16: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 17:23:16: #1 total tags in treatment: 1786101 INFO @ Sun, 21 Jun 2020 17:23:16: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:23:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:23:17: #1 tags after filtering in treatment: 1785494 INFO @ Sun, 21 Jun 2020 17:23:17: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:23:17: #1 finished! INFO @ Sun, 21 Jun 2020 17:23:17: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:23:17: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:23:17: #2 number of paired peaks: 4991 INFO @ Sun, 21 Jun 2020 17:23:17: start model_add_line... INFO @ Sun, 21 Jun 2020 17:23:17: start X-correlation... INFO @ Sun, 21 Jun 2020 17:23:17: end of X-cor INFO @ Sun, 21 Jun 2020 17:23:17: #2 finished! INFO @ Sun, 21 Jun 2020 17:23:17: #2 predicted fragment length is 153 bps INFO @ Sun, 21 Jun 2020 17:23:17: #2 alternative fragment length(s) may be 153 bps INFO @ Sun, 21 Jun 2020 17:23:17: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX1038029/SRX1038029.10_model.r INFO @ Sun, 21 Jun 2020 17:23:17: #3 Call peaks... INFO @ Sun, 21 Jun 2020 17:23:17: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 21 Jun 2020 17:23:22: #3 Call peaks for each chromosome... INFO @ Sun, 21 Jun 2020 17:23:25: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX1038029/SRX1038029.10_peaks.xls INFO @ Sun, 21 Jun 2020 17:23:25: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX1038029/SRX1038029.10_peaks.narrowPeak INFO @ Sun, 21 Jun 2020 17:23:25: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX1038029/SRX1038029.10_summits.bed INFO @ Sun, 21 Jun 2020 17:23:25: Done! pass1 - making usageList (75 chroms): 1 millis pass2 - checking and writing primary data (1178 records, 4 fields): 6 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:23:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1038029/SRX1038029.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1038029/SRX1038029.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1038029/SRX1038029.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1038029/SRX1038029.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:23:33: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:23:33: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:23:39: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:23:44: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 17:23:44: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 17:23:44: #1 total tags in treatment: 1786101 INFO @ Sun, 21 Jun 2020 17:23:44: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:23:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:23:44: #1 tags after filtering in treatment: 1785494 INFO @ Sun, 21 Jun 2020 17:23:44: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:23:44: #1 finished! INFO @ Sun, 21 Jun 2020 17:23:44: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:23:44: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 17:23:44: #2 number of paired peaks: 4991 INFO @ Sun, 21 Jun 2020 17:23:44: start model_add_line... INFO @ Sun, 21 Jun 2020 17:23:44: start X-correlation... INFO @ Sun, 21 Jun 2020 17:23:44: end of X-cor INFO @ Sun, 21 Jun 2020 17:23:44: #2 finished! INFO @ Sun, 21 Jun 2020 17:23:44: #2 predicted fragment length is 153 bps INFO @ Sun, 21 Jun 2020 17:23:44: #2 alternative fragment length(s) may be 153 bps INFO @ Sun, 21 Jun 2020 17:23:44: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX1038029/SRX1038029.20_model.r INFO @ Sun, 21 Jun 2020 17:23:44: #3 Call peaks... INFO @ Sun, 21 Jun 2020 17:23:44: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 21 Jun 2020 17:23:50: #3 Call peaks for each chromosome... INFO @ Sun, 21 Jun 2020 17:23:52: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX1038029/SRX1038029.20_peaks.xls INFO @ Sun, 21 Jun 2020 17:23:52: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX1038029/SRX1038029.20_peaks.narrowPeak INFO @ Sun, 21 Jun 2020 17:23:52: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX1038029/SRX1038029.20_summits.bed INFO @ Sun, 21 Jun 2020 17:23:52: Done! pass1 - making usageList (51 chroms): 0 millis pass2 - checking and writing primary data (699 records, 4 fields): 5 millis CompletedMACS2peakCalling