Job ID = 14172572 SRX = SRX10089760 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:10 27506975 reads; of these: 27506975 (100.00%) were unpaired; of these: 3014344 (10.96%) aligned 0 times 21040336 (76.49%) aligned exactly 1 time 3452295 (12.55%) aligned >1 times 89.04% overall alignment rate Time searching: 00:05:10 Overall time: 00:05:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5577772 / 24492631 = 0.2277 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 16:08:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX10089760/SRX10089760.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX10089760/SRX10089760.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX10089760/SRX10089760.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX10089760/SRX10089760.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 16:08:48: #1 read tag files... INFO @ Sat, 11 Dec 2021 16:08:48: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 16:08:53: 1000000 INFO @ Sat, 11 Dec 2021 16:08:58: 2000000 INFO @ Sat, 11 Dec 2021 16:09:03: 3000000 INFO @ Sat, 11 Dec 2021 16:09:08: 4000000 INFO @ Sat, 11 Dec 2021 16:09:13: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 16:09:18: 6000000 INFO @ Sat, 11 Dec 2021 16:09:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX10089760/SRX10089760.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX10089760/SRX10089760.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX10089760/SRX10089760.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX10089760/SRX10089760.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 16:09:18: #1 read tag files... INFO @ Sat, 11 Dec 2021 16:09:18: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 16:09:23: 7000000 INFO @ Sat, 11 Dec 2021 16:09:24: 1000000 INFO @ Sat, 11 Dec 2021 16:09:28: 8000000 INFO @ Sat, 11 Dec 2021 16:09:29: 2000000 INFO @ Sat, 11 Dec 2021 16:09:33: 9000000 INFO @ Sat, 11 Dec 2021 16:09:34: 3000000 INFO @ Sat, 11 Dec 2021 16:09:39: 10000000 INFO @ Sat, 11 Dec 2021 16:09:40: 4000000 INFO @ Sat, 11 Dec 2021 16:09:44: 11000000 INFO @ Sat, 11 Dec 2021 16:09:45: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 16:09:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX10089760/SRX10089760.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX10089760/SRX10089760.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX10089760/SRX10089760.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX10089760/SRX10089760.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 16:09:49: #1 read tag files... INFO @ Sat, 11 Dec 2021 16:09:49: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 16:09:49: 12000000 INFO @ Sat, 11 Dec 2021 16:09:50: 6000000 INFO @ Sat, 11 Dec 2021 16:09:55: 13000000 INFO @ Sat, 11 Dec 2021 16:09:55: 1000000 INFO @ Sat, 11 Dec 2021 16:09:56: 7000000 INFO @ Sat, 11 Dec 2021 16:10:01: 14000000 INFO @ Sat, 11 Dec 2021 16:10:02: 2000000 INFO @ Sat, 11 Dec 2021 16:10:02: 8000000 INFO @ Sat, 11 Dec 2021 16:10:07: 15000000 INFO @ Sat, 11 Dec 2021 16:10:07: 9000000 INFO @ Sat, 11 Dec 2021 16:10:08: 3000000 INFO @ Sat, 11 Dec 2021 16:10:13: 16000000 INFO @ Sat, 11 Dec 2021 16:10:13: 10000000 INFO @ Sat, 11 Dec 2021 16:10:14: 4000000 INFO @ Sat, 11 Dec 2021 16:10:18: 17000000 INFO @ Sat, 11 Dec 2021 16:10:19: 11000000 INFO @ Sat, 11 Dec 2021 16:10:21: 5000000 INFO @ Sat, 11 Dec 2021 16:10:24: 18000000 INFO @ Sat, 11 Dec 2021 16:10:25: 12000000 INFO @ Sat, 11 Dec 2021 16:10:27: 6000000 INFO @ Sat, 11 Dec 2021 16:10:30: #1 tag size is determined as 40 bps INFO @ Sat, 11 Dec 2021 16:10:30: #1 tag size = 40 INFO @ Sat, 11 Dec 2021 16:10:30: #1 total tags in treatment: 18914859 INFO @ Sat, 11 Dec 2021 16:10:30: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 16:10:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 16:10:30: #1 tags after filtering in treatment: 18914859 INFO @ Sat, 11 Dec 2021 16:10:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 16:10:30: #1 finished! INFO @ Sat, 11 Dec 2021 16:10:30: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 16:10:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 16:10:30: 13000000 INFO @ Sat, 11 Dec 2021 16:10:32: #2 number of paired peaks: 1421 INFO @ Sat, 11 Dec 2021 16:10:32: start model_add_line... INFO @ Sat, 11 Dec 2021 16:10:32: start X-correlation... INFO @ Sat, 11 Dec 2021 16:10:32: end of X-cor INFO @ Sat, 11 Dec 2021 16:10:32: #2 finished! INFO @ Sat, 11 Dec 2021 16:10:32: #2 predicted fragment length is 195 bps INFO @ Sat, 11 Dec 2021 16:10:32: #2 alternative fragment length(s) may be 195 bps INFO @ Sat, 11 Dec 2021 16:10:32: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX10089760/SRX10089760.05_model.r INFO @ Sat, 11 Dec 2021 16:10:32: #3 Call peaks... INFO @ Sat, 11 Dec 2021 16:10:32: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 16:10:34: 7000000 INFO @ Sat, 11 Dec 2021 16:10:36: 14000000 INFO @ Sat, 11 Dec 2021 16:10:40: 8000000 INFO @ Sat, 11 Dec 2021 16:10:41: 15000000 INFO @ Sat, 11 Dec 2021 16:10:46: 9000000 INFO @ Sat, 11 Dec 2021 16:10:47: 16000000 INFO @ Sat, 11 Dec 2021 16:10:53: 10000000 INFO @ Sat, 11 Dec 2021 16:10:53: 17000000 INFO @ Sat, 11 Dec 2021 16:10:59: 18000000 INFO @ Sat, 11 Dec 2021 16:10:59: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 16:11:04: #1 tag size is determined as 40 bps INFO @ Sat, 11 Dec 2021 16:11:04: #1 tag size = 40 INFO @ Sat, 11 Dec 2021 16:11:04: #1 total tags in treatment: 18914859 INFO @ Sat, 11 Dec 2021 16:11:04: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 16:11:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 16:11:05: #1 tags after filtering in treatment: 18914859 INFO @ Sat, 11 Dec 2021 16:11:05: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 16:11:05: #1 finished! INFO @ Sat, 11 Dec 2021 16:11:05: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 16:11:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 16:11:05: 12000000 INFO @ Sat, 11 Dec 2021 16:11:06: #2 number of paired peaks: 1421 INFO @ Sat, 11 Dec 2021 16:11:06: start model_add_line... INFO @ Sat, 11 Dec 2021 16:11:06: start X-correlation... INFO @ Sat, 11 Dec 2021 16:11:06: end of X-cor INFO @ Sat, 11 Dec 2021 16:11:06: #2 finished! INFO @ Sat, 11 Dec 2021 16:11:06: #2 predicted fragment length is 195 bps INFO @ Sat, 11 Dec 2021 16:11:06: #2 alternative fragment length(s) may be 195 bps INFO @ Sat, 11 Dec 2021 16:11:06: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX10089760/SRX10089760.10_model.r INFO @ Sat, 11 Dec 2021 16:11:06: #3 Call peaks... INFO @ Sat, 11 Dec 2021 16:11:06: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 16:11:12: 13000000 INFO @ Sat, 11 Dec 2021 16:11:14: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 16:11:18: 14000000 INFO @ Sat, 11 Dec 2021 16:11:24: 15000000 INFO @ Sat, 11 Dec 2021 16:11:30: 16000000 INFO @ Sat, 11 Dec 2021 16:11:35: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX10089760/SRX10089760.05_peaks.xls INFO @ Sat, 11 Dec 2021 16:11:35: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX10089760/SRX10089760.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 16:11:35: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX10089760/SRX10089760.05_summits.bed INFO @ Sat, 11 Dec 2021 16:11:35: Done! pass1 - making usageList (455 chroms): 2 millis pass2 - checking and writing primary data (10895 records, 4 fields): 22 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 16:11:36: 17000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 16:11:41: 18000000 INFO @ Sat, 11 Dec 2021 16:11:47: #1 tag size is determined as 40 bps INFO @ Sat, 11 Dec 2021 16:11:47: #1 tag size = 40 INFO @ Sat, 11 Dec 2021 16:11:47: #1 total tags in treatment: 18914859 INFO @ Sat, 11 Dec 2021 16:11:47: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 16:11:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 16:11:48: #1 tags after filtering in treatment: 18914859 INFO @ Sat, 11 Dec 2021 16:11:48: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 16:11:48: #1 finished! INFO @ Sat, 11 Dec 2021 16:11:48: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 16:11:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 16:11:49: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 16:11:49: #2 number of paired peaks: 1421 INFO @ Sat, 11 Dec 2021 16:11:49: start model_add_line... INFO @ Sat, 11 Dec 2021 16:11:49: start X-correlation... INFO @ Sat, 11 Dec 2021 16:11:49: end of X-cor INFO @ Sat, 11 Dec 2021 16:11:49: #2 finished! INFO @ Sat, 11 Dec 2021 16:11:49: #2 predicted fragment length is 195 bps INFO @ Sat, 11 Dec 2021 16:11:49: #2 alternative fragment length(s) may be 195 bps INFO @ Sat, 11 Dec 2021 16:11:49: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX10089760/SRX10089760.20_model.r INFO @ Sat, 11 Dec 2021 16:11:49: #3 Call peaks... INFO @ Sat, 11 Dec 2021 16:11:49: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 16:12:09: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX10089760/SRX10089760.10_peaks.xls INFO @ Sat, 11 Dec 2021 16:12:09: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX10089760/SRX10089760.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 16:12:09: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX10089760/SRX10089760.10_summits.bed INFO @ Sat, 11 Dec 2021 16:12:09: Done! pass1 - making usageList (372 chroms): 2 millis pass2 - checking and writing primary data (7931 records, 4 fields): 18 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 16:12:32: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 16:12:52: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX10089760/SRX10089760.20_peaks.xls INFO @ Sat, 11 Dec 2021 16:12:52: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX10089760/SRX10089760.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 16:12:52: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX10089760/SRX10089760.20_summits.bed INFO @ Sat, 11 Dec 2021 16:12:52: Done! pass1 - making usageList (193 chroms): 1 millis pass2 - checking and writing primary data (4685 records, 4 fields): 11 millis CompletedMACS2peakCalling