Job ID = 14172520 SRX = SRX10089759 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:36 26395075 reads; of these: 26395075 (100.00%) were unpaired; of these: 3727757 (14.12%) aligned 0 times 19248557 (72.92%) aligned exactly 1 time 3418761 (12.95%) aligned >1 times 85.88% overall alignment rate Time searching: 00:04:36 Overall time: 00:04:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5240965 / 22667318 = 0.2312 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:55:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX10089759/SRX10089759.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX10089759/SRX10089759.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX10089759/SRX10089759.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX10089759/SRX10089759.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:55:51: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:55:51: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:55:55: 1000000 INFO @ Sat, 11 Dec 2021 15:56:00: 2000000 INFO @ Sat, 11 Dec 2021 15:56:04: 3000000 INFO @ Sat, 11 Dec 2021 15:56:08: 4000000 INFO @ Sat, 11 Dec 2021 15:56:13: 5000000 INFO @ Sat, 11 Dec 2021 15:56:17: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:56:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX10089759/SRX10089759.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX10089759/SRX10089759.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX10089759/SRX10089759.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX10089759/SRX10089759.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:56:21: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:56:21: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:56:21: 7000000 INFO @ Sat, 11 Dec 2021 15:56:25: 1000000 INFO @ Sat, 11 Dec 2021 15:56:26: 8000000 INFO @ Sat, 11 Dec 2021 15:56:29: 2000000 INFO @ Sat, 11 Dec 2021 15:56:30: 9000000 INFO @ Sat, 11 Dec 2021 15:56:34: 3000000 INFO @ Sat, 11 Dec 2021 15:56:35: 10000000 INFO @ Sat, 11 Dec 2021 15:56:38: 4000000 INFO @ Sat, 11 Dec 2021 15:56:39: 11000000 INFO @ Sat, 11 Dec 2021 15:56:42: 5000000 INFO @ Sat, 11 Dec 2021 15:56:43: 12000000 INFO @ Sat, 11 Dec 2021 15:56:47: 6000000 INFO @ Sat, 11 Dec 2021 15:56:48: 13000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:56:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX10089759/SRX10089759.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX10089759/SRX10089759.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX10089759/SRX10089759.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX10089759/SRX10089759.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:56:51: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:56:51: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:56:51: 7000000 INFO @ Sat, 11 Dec 2021 15:56:52: 14000000 INFO @ Sat, 11 Dec 2021 15:56:55: 8000000 INFO @ Sat, 11 Dec 2021 15:56:56: 1000000 INFO @ Sat, 11 Dec 2021 15:56:57: 15000000 INFO @ Sat, 11 Dec 2021 15:57:00: 9000000 INFO @ Sat, 11 Dec 2021 15:57:01: 16000000 INFO @ Sat, 11 Dec 2021 15:57:01: 2000000 INFO @ Sat, 11 Dec 2021 15:57:04: 10000000 INFO @ Sat, 11 Dec 2021 15:57:05: 17000000 INFO @ Sat, 11 Dec 2021 15:57:06: 3000000 INFO @ Sat, 11 Dec 2021 15:57:07: #1 tag size is determined as 40 bps INFO @ Sat, 11 Dec 2021 15:57:07: #1 tag size = 40 INFO @ Sat, 11 Dec 2021 15:57:07: #1 total tags in treatment: 17426353 INFO @ Sat, 11 Dec 2021 15:57:07: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:57:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:57:08: #1 tags after filtering in treatment: 17426353 INFO @ Sat, 11 Dec 2021 15:57:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 15:57:08: #1 finished! INFO @ Sat, 11 Dec 2021 15:57:08: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:57:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:57:08: 11000000 INFO @ Sat, 11 Dec 2021 15:57:09: #2 number of paired peaks: 1206 INFO @ Sat, 11 Dec 2021 15:57:09: start model_add_line... INFO @ Sat, 11 Dec 2021 15:57:09: start X-correlation... INFO @ Sat, 11 Dec 2021 15:57:09: end of X-cor INFO @ Sat, 11 Dec 2021 15:57:09: #2 finished! INFO @ Sat, 11 Dec 2021 15:57:09: #2 predicted fragment length is 194 bps INFO @ Sat, 11 Dec 2021 15:57:09: #2 alternative fragment length(s) may be 194 bps INFO @ Sat, 11 Dec 2021 15:57:09: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX10089759/SRX10089759.05_model.r INFO @ Sat, 11 Dec 2021 15:57:09: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:57:09: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 15:57:11: 4000000 INFO @ Sat, 11 Dec 2021 15:57:13: 12000000 INFO @ Sat, 11 Dec 2021 15:57:16: 5000000 INFO @ Sat, 11 Dec 2021 15:57:17: 13000000 INFO @ Sat, 11 Dec 2021 15:57:21: 6000000 INFO @ Sat, 11 Dec 2021 15:57:21: 14000000 INFO @ Sat, 11 Dec 2021 15:57:26: 15000000 INFO @ Sat, 11 Dec 2021 15:57:26: 7000000 INFO @ Sat, 11 Dec 2021 15:57:30: 16000000 INFO @ Sat, 11 Dec 2021 15:57:31: 8000000 INFO @ Sat, 11 Dec 2021 15:57:35: 17000000 INFO @ Sat, 11 Dec 2021 15:57:36: 9000000 INFO @ Sat, 11 Dec 2021 15:57:37: #1 tag size is determined as 40 bps INFO @ Sat, 11 Dec 2021 15:57:37: #1 tag size = 40 INFO @ Sat, 11 Dec 2021 15:57:37: #1 total tags in treatment: 17426353 INFO @ Sat, 11 Dec 2021 15:57:37: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:57:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:57:38: #1 tags after filtering in treatment: 17426353 INFO @ Sat, 11 Dec 2021 15:57:38: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 15:57:38: #1 finished! INFO @ Sat, 11 Dec 2021 15:57:38: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:57:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:57:39: #2 number of paired peaks: 1206 INFO @ Sat, 11 Dec 2021 15:57:39: start model_add_line... INFO @ Sat, 11 Dec 2021 15:57:39: start X-correlation... INFO @ Sat, 11 Dec 2021 15:57:39: end of X-cor INFO @ Sat, 11 Dec 2021 15:57:39: #2 finished! INFO @ Sat, 11 Dec 2021 15:57:39: #2 predicted fragment length is 194 bps INFO @ Sat, 11 Dec 2021 15:57:39: #2 alternative fragment length(s) may be 194 bps INFO @ Sat, 11 Dec 2021 15:57:39: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX10089759/SRX10089759.10_model.r INFO @ Sat, 11 Dec 2021 15:57:39: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:57:39: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 15:57:41: 10000000 INFO @ Sat, 11 Dec 2021 15:57:46: 11000000 INFO @ Sat, 11 Dec 2021 15:57:48: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 15:57:51: 12000000 INFO @ Sat, 11 Dec 2021 15:57:56: 13000000 INFO @ Sat, 11 Dec 2021 15:58:01: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 15:58:06: 15000000 INFO @ Sat, 11 Dec 2021 15:58:07: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX10089759/SRX10089759.05_peaks.xls INFO @ Sat, 11 Dec 2021 15:58:07: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX10089759/SRX10089759.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:58:07: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX10089759/SRX10089759.05_summits.bed INFO @ Sat, 11 Dec 2021 15:58:07: Done! pass1 - making usageList (426 chroms): 2 millis pass2 - checking and writing primary data (10116 records, 4 fields): 20 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 15:58:11: 16000000 INFO @ Sat, 11 Dec 2021 15:58:16: 17000000 INFO @ Sat, 11 Dec 2021 15:58:17: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 15:58:19: #1 tag size is determined as 40 bps INFO @ Sat, 11 Dec 2021 15:58:19: #1 tag size = 40 INFO @ Sat, 11 Dec 2021 15:58:19: #1 total tags in treatment: 17426353 INFO @ Sat, 11 Dec 2021 15:58:19: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:58:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:58:19: #1 tags after filtering in treatment: 17426353 INFO @ Sat, 11 Dec 2021 15:58:19: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 15:58:19: #1 finished! INFO @ Sat, 11 Dec 2021 15:58:19: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:58:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:58:20: #2 number of paired peaks: 1206 INFO @ Sat, 11 Dec 2021 15:58:20: start model_add_line... INFO @ Sat, 11 Dec 2021 15:58:20: start X-correlation... INFO @ Sat, 11 Dec 2021 15:58:20: end of X-cor INFO @ Sat, 11 Dec 2021 15:58:20: #2 finished! INFO @ Sat, 11 Dec 2021 15:58:20: #2 predicted fragment length is 194 bps INFO @ Sat, 11 Dec 2021 15:58:20: #2 alternative fragment length(s) may be 194 bps INFO @ Sat, 11 Dec 2021 15:58:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX10089759/SRX10089759.20_model.r INFO @ Sat, 11 Dec 2021 15:58:20: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:58:20: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 15:58:35: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX10089759/SRX10089759.10_peaks.xls INFO @ Sat, 11 Dec 2021 15:58:36: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX10089759/SRX10089759.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:58:36: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX10089759/SRX10089759.10_summits.bed INFO @ Sat, 11 Dec 2021 15:58:36: Done! BigWig に変換しました。 pass1 - making usageList (307 chroms): 1 millis pass2 - checking and writing primary data (6860 records, 4 fields): 15 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 15:58:56: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 15:59:14: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX10089759/SRX10089759.20_peaks.xls INFO @ Sat, 11 Dec 2021 15:59:14: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX10089759/SRX10089759.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:59:14: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX10089759/SRX10089759.20_summits.bed INFO @ Sat, 11 Dec 2021 15:59:14: Done! pass1 - making usageList (123 chroms): 1 millis pass2 - checking and writing primary data (3841 records, 4 fields): 9 millis CompletedMACS2peakCalling