Job ID = 6453022 SRX = SRX099861 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:04:08 prefetch.2.10.7: 1) Downloading 'SRR350870'... 2020-06-21T08:04:08 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:09:22 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:09:22 prefetch.2.10.7: 1) 'SRR350870' was downloaded successfully Read 22026834 spots for SRR350870/SRR350870.sra Written 22026834 spots for SRR350870/SRR350870.sra 2020-06-21T08:10:33 prefetch.2.10.7: 1) Downloading 'SRR350871'... 2020-06-21T08:10:33 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:20:57 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:20:57 prefetch.2.10.7: 1) 'SRR350871' was downloaded successfully Read 40753322 spots for SRR350871/SRR350871.sra Written 40753322 spots for SRR350871/SRR350871.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:14 62780156 reads; of these: 62780156 (100.00%) were unpaired; of these: 8035956 (12.80%) aligned 0 times 45805256 (72.96%) aligned exactly 1 time 8938944 (14.24%) aligned >1 times 87.20% overall alignment rate Time searching: 00:12:14 Overall time: 00:12:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 19728489 / 54744200 = 0.3604 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:46:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX099861/SRX099861.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX099861/SRX099861.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX099861/SRX099861.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX099861/SRX099861.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:46:39: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:46:39: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:46:46: 1000000 INFO @ Sun, 21 Jun 2020 17:46:52: 2000000 INFO @ Sun, 21 Jun 2020 17:46:59: 3000000 INFO @ Sun, 21 Jun 2020 17:47:06: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:47:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX099861/SRX099861.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX099861/SRX099861.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX099861/SRX099861.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX099861/SRX099861.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:47:09: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:47:09: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:47:13: 5000000 INFO @ Sun, 21 Jun 2020 17:47:16: 1000000 INFO @ Sun, 21 Jun 2020 17:47:20: 6000000 INFO @ Sun, 21 Jun 2020 17:47:24: 2000000 INFO @ Sun, 21 Jun 2020 17:47:27: 7000000 INFO @ Sun, 21 Jun 2020 17:47:31: 3000000 INFO @ Sun, 21 Jun 2020 17:47:35: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:47:39: 4000000 INFO @ Sun, 21 Jun 2020 17:47:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX099861/SRX099861.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX099861/SRX099861.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX099861/SRX099861.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX099861/SRX099861.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:47:39: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:47:39: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:47:42: 9000000 INFO @ Sun, 21 Jun 2020 17:47:46: 1000000 INFO @ Sun, 21 Jun 2020 17:47:46: 5000000 INFO @ Sun, 21 Jun 2020 17:47:49: 10000000 INFO @ Sun, 21 Jun 2020 17:47:53: 2000000 INFO @ Sun, 21 Jun 2020 17:47:53: 6000000 INFO @ Sun, 21 Jun 2020 17:47:57: 11000000 INFO @ Sun, 21 Jun 2020 17:47:59: 3000000 INFO @ Sun, 21 Jun 2020 17:48:01: 7000000 INFO @ Sun, 21 Jun 2020 17:48:04: 12000000 INFO @ Sun, 21 Jun 2020 17:48:06: 4000000 INFO @ Sun, 21 Jun 2020 17:48:08: 8000000 INFO @ Sun, 21 Jun 2020 17:48:11: 13000000 INFO @ Sun, 21 Jun 2020 17:48:13: 5000000 INFO @ Sun, 21 Jun 2020 17:48:16: 9000000 INFO @ Sun, 21 Jun 2020 17:48:19: 14000000 INFO @ Sun, 21 Jun 2020 17:48:19: 6000000 INFO @ Sun, 21 Jun 2020 17:48:23: 10000000 INFO @ Sun, 21 Jun 2020 17:48:26: 15000000 INFO @ Sun, 21 Jun 2020 17:48:26: 7000000 INFO @ Sun, 21 Jun 2020 17:48:31: 11000000 INFO @ Sun, 21 Jun 2020 17:48:33: 8000000 INFO @ Sun, 21 Jun 2020 17:48:33: 16000000 INFO @ Sun, 21 Jun 2020 17:48:38: 12000000 INFO @ Sun, 21 Jun 2020 17:48:40: 9000000 INFO @ Sun, 21 Jun 2020 17:48:41: 17000000 INFO @ Sun, 21 Jun 2020 17:48:45: 13000000 INFO @ Sun, 21 Jun 2020 17:48:46: 10000000 INFO @ Sun, 21 Jun 2020 17:48:48: 18000000 INFO @ Sun, 21 Jun 2020 17:48:52: 14000000 INFO @ Sun, 21 Jun 2020 17:48:53: 11000000 INFO @ Sun, 21 Jun 2020 17:48:55: 19000000 INFO @ Sun, 21 Jun 2020 17:49:00: 15000000 INFO @ Sun, 21 Jun 2020 17:49:00: 12000000 INFO @ Sun, 21 Jun 2020 17:49:03: 20000000 INFO @ Sun, 21 Jun 2020 17:49:07: 13000000 INFO @ Sun, 21 Jun 2020 17:49:07: 16000000 INFO @ Sun, 21 Jun 2020 17:49:10: 21000000 INFO @ Sun, 21 Jun 2020 17:49:14: 14000000 INFO @ Sun, 21 Jun 2020 17:49:14: 17000000 INFO @ Sun, 21 Jun 2020 17:49:17: 22000000 INFO @ Sun, 21 Jun 2020 17:49:20: 15000000 INFO @ Sun, 21 Jun 2020 17:49:22: 18000000 INFO @ Sun, 21 Jun 2020 17:49:25: 23000000 INFO @ Sun, 21 Jun 2020 17:49:27: 16000000 INFO @ Sun, 21 Jun 2020 17:49:29: 19000000 INFO @ Sun, 21 Jun 2020 17:49:32: 24000000 INFO @ Sun, 21 Jun 2020 17:49:34: 17000000 INFO @ Sun, 21 Jun 2020 17:49:36: 20000000 INFO @ Sun, 21 Jun 2020 17:49:39: 25000000 INFO @ Sun, 21 Jun 2020 17:49:41: 18000000 INFO @ Sun, 21 Jun 2020 17:49:44: 21000000 INFO @ Sun, 21 Jun 2020 17:49:47: 26000000 INFO @ Sun, 21 Jun 2020 17:49:47: 19000000 INFO @ Sun, 21 Jun 2020 17:49:51: 22000000 INFO @ Sun, 21 Jun 2020 17:49:54: 27000000 INFO @ Sun, 21 Jun 2020 17:49:54: 20000000 INFO @ Sun, 21 Jun 2020 17:49:59: 23000000 INFO @ Sun, 21 Jun 2020 17:50:01: 28000000 INFO @ Sun, 21 Jun 2020 17:50:01: 21000000 INFO @ Sun, 21 Jun 2020 17:50:06: 24000000 INFO @ Sun, 21 Jun 2020 17:50:08: 22000000 INFO @ Sun, 21 Jun 2020 17:50:09: 29000000 INFO @ Sun, 21 Jun 2020 17:50:13: 25000000 INFO @ Sun, 21 Jun 2020 17:50:15: 23000000 INFO @ Sun, 21 Jun 2020 17:50:16: 30000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:50:21: 26000000 INFO @ Sun, 21 Jun 2020 17:50:21: 24000000 INFO @ Sun, 21 Jun 2020 17:50:24: 31000000 INFO @ Sun, 21 Jun 2020 17:50:28: 25000000 INFO @ Sun, 21 Jun 2020 17:50:28: 27000000 INFO @ Sun, 21 Jun 2020 17:50:31: 32000000 INFO @ Sun, 21 Jun 2020 17:50:35: 26000000 INFO @ Sun, 21 Jun 2020 17:50:35: 28000000 INFO @ Sun, 21 Jun 2020 17:50:39: 33000000 INFO @ Sun, 21 Jun 2020 17:50:42: 27000000 INFO @ Sun, 21 Jun 2020 17:50:43: 29000000 INFO @ Sun, 21 Jun 2020 17:50:46: 34000000 INFO @ Sun, 21 Jun 2020 17:50:49: 28000000 INFO @ Sun, 21 Jun 2020 17:50:51: 30000000 INFO @ Sun, 21 Jun 2020 17:50:54: 35000000 INFO @ Sun, 21 Jun 2020 17:50:54: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:50:54: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:50:54: #1 total tags in treatment: 35015711 INFO @ Sun, 21 Jun 2020 17:50:54: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:50:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:50:55: #1 tags after filtering in treatment: 35015709 INFO @ Sun, 21 Jun 2020 17:50:55: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:50:55: #1 finished! INFO @ Sun, 21 Jun 2020 17:50:55: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:50:55: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:50:56: 29000000 INFO @ Sun, 21 Jun 2020 17:50:57: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 17:50:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:50:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX099861/SRX099861.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX099861/SRX099861.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX099861/SRX099861.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX099861/SRX099861.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:50:58: 31000000 INFO @ Sun, 21 Jun 2020 17:51:03: 30000000 INFO @ Sun, 21 Jun 2020 17:51:05: 32000000 INFO @ Sun, 21 Jun 2020 17:51:09: 31000000 INFO @ Sun, 21 Jun 2020 17:51:13: 33000000 INFO @ Sun, 21 Jun 2020 17:51:16: 32000000 INFO @ Sun, 21 Jun 2020 17:51:20: 34000000 INFO @ Sun, 21 Jun 2020 17:51:23: 33000000 INFO @ Sun, 21 Jun 2020 17:51:28: 35000000 INFO @ Sun, 21 Jun 2020 17:51:28: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:51:28: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:51:28: #1 total tags in treatment: 35015711 INFO @ Sun, 21 Jun 2020 17:51:28: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:51:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:51:29: #1 tags after filtering in treatment: 35015709 INFO @ Sun, 21 Jun 2020 17:51:29: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:51:29: #1 finished! INFO @ Sun, 21 Jun 2020 17:51:29: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:51:29: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:51:29: 34000000 INFO @ Sun, 21 Jun 2020 17:51:31: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 17:51:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:51:31: Process for pairing-model is terminated! BigWig に変換しました。 cut: /home/okishinya/chipatlas/results/dm6/SRX099861/SRX099861.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX099861/SRX099861.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX099861/SRX099861.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX099861/SRX099861.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:51:35: 35000000 INFO @ Sun, 21 Jun 2020 17:51:36: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:51:36: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:51:36: #1 total tags in treatment: 35015711 INFO @ Sun, 21 Jun 2020 17:51:36: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:51:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:51:37: #1 tags after filtering in treatment: 35015709 INFO @ Sun, 21 Jun 2020 17:51:37: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:51:37: #1 finished! INFO @ Sun, 21 Jun 2020 17:51:37: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:51:37: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:51:39: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 17:51:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:51:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX099861/SRX099861.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX099861/SRX099861.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX099861/SRX099861.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX099861/SRX099861.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling