Job ID = 6529210 SRX = SRX042246 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:29 32940343 reads; of these: 32940343 (100.00%) were unpaired; of these: 9021340 (27.39%) aligned 0 times 20451521 (62.09%) aligned exactly 1 time 3467482 (10.53%) aligned >1 times 72.61% overall alignment rate Time searching: 00:11:30 Overall time: 00:11:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7595088 / 23919003 = 0.3175 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:43:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX042246/SRX042246.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX042246/SRX042246.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX042246/SRX042246.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX042246/SRX042246.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:43:10: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:43:10: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:43:18: 1000000 INFO @ Tue, 30 Jun 2020 01:43:26: 2000000 INFO @ Tue, 30 Jun 2020 01:43:34: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:43:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX042246/SRX042246.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX042246/SRX042246.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX042246/SRX042246.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX042246/SRX042246.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:43:40: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:43:40: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:43:42: 4000000 INFO @ Tue, 30 Jun 2020 01:43:49: 1000000 INFO @ Tue, 30 Jun 2020 01:43:52: 5000000 INFO @ Tue, 30 Jun 2020 01:43:59: 2000000 INFO @ Tue, 30 Jun 2020 01:44:01: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:44:09: 3000000 INFO @ Tue, 30 Jun 2020 01:44:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX042246/SRX042246.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX042246/SRX042246.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX042246/SRX042246.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX042246/SRX042246.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:44:10: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:44:10: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:44:10: 7000000 INFO @ Tue, 30 Jun 2020 01:44:19: 4000000 INFO @ Tue, 30 Jun 2020 01:44:20: 8000000 INFO @ Tue, 30 Jun 2020 01:44:21: 1000000 INFO @ Tue, 30 Jun 2020 01:44:30: 5000000 INFO @ Tue, 30 Jun 2020 01:44:30: 9000000 INFO @ Tue, 30 Jun 2020 01:44:31: 2000000 INFO @ Tue, 30 Jun 2020 01:44:39: 10000000 INFO @ Tue, 30 Jun 2020 01:44:40: 6000000 INFO @ Tue, 30 Jun 2020 01:44:42: 3000000 INFO @ Tue, 30 Jun 2020 01:44:48: 11000000 INFO @ Tue, 30 Jun 2020 01:44:50: 7000000 INFO @ Tue, 30 Jun 2020 01:44:52: 4000000 INFO @ Tue, 30 Jun 2020 01:44:58: 12000000 INFO @ Tue, 30 Jun 2020 01:45:01: 8000000 INFO @ Tue, 30 Jun 2020 01:45:03: 5000000 INFO @ Tue, 30 Jun 2020 01:45:07: 13000000 INFO @ Tue, 30 Jun 2020 01:45:11: 9000000 INFO @ Tue, 30 Jun 2020 01:45:13: 6000000 INFO @ Tue, 30 Jun 2020 01:45:16: 14000000 INFO @ Tue, 30 Jun 2020 01:45:21: 10000000 INFO @ Tue, 30 Jun 2020 01:45:23: 7000000 INFO @ Tue, 30 Jun 2020 01:45:24: 15000000 INFO @ Tue, 30 Jun 2020 01:45:31: 11000000 INFO @ Tue, 30 Jun 2020 01:45:32: 16000000 INFO @ Tue, 30 Jun 2020 01:45:34: 8000000 INFO @ Tue, 30 Jun 2020 01:45:35: #1 tag size is determined as 76 bps INFO @ Tue, 30 Jun 2020 01:45:35: #1 tag size = 76 INFO @ Tue, 30 Jun 2020 01:45:35: #1 total tags in treatment: 16323915 INFO @ Tue, 30 Jun 2020 01:45:35: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:45:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:45:35: #1 tags after filtering in treatment: 16323766 INFO @ Tue, 30 Jun 2020 01:45:35: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:45:35: #1 finished! INFO @ Tue, 30 Jun 2020 01:45:35: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:45:35: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:45:37: #2 number of paired peaks: 463 WARNING @ Tue, 30 Jun 2020 01:45:37: Fewer paired peaks (463) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 463 pairs to build model! INFO @ Tue, 30 Jun 2020 01:45:37: start model_add_line... INFO @ Tue, 30 Jun 2020 01:45:37: start X-correlation... INFO @ Tue, 30 Jun 2020 01:45:37: end of X-cor INFO @ Tue, 30 Jun 2020 01:45:37: #2 finished! INFO @ Tue, 30 Jun 2020 01:45:37: #2 predicted fragment length is 0 bps INFO @ Tue, 30 Jun 2020 01:45:37: #2 alternative fragment length(s) may be 0 bps INFO @ Tue, 30 Jun 2020 01:45:37: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX042246/SRX042246.05_model.r WARNING @ Tue, 30 Jun 2020 01:45:37: #2 Since the d (0) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 30 Jun 2020 01:45:37: #2 You may need to consider one of the other alternative d(s): 0 WARNING @ Tue, 30 Jun 2020 01:45:37: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 30 Jun 2020 01:45:37: #3 Call peaks... INFO @ Tue, 30 Jun 2020 01:45:37: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 30 Jun 2020 01:45:41: 12000000 INFO @ Tue, 30 Jun 2020 01:45:44: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 01:45:52: 13000000 INFO @ Tue, 30 Jun 2020 01:45:55: 10000000 INFO @ Tue, 30 Jun 2020 01:46:03: 14000000 INFO @ Tue, 30 Jun 2020 01:46:05: 11000000 INFO @ Tue, 30 Jun 2020 01:46:13: 15000000 INFO @ Tue, 30 Jun 2020 01:46:15: 12000000 INFO @ Tue, 30 Jun 2020 01:46:24: 16000000 INFO @ Tue, 30 Jun 2020 01:46:26: 13000000 INFO @ Tue, 30 Jun 2020 01:46:27: #1 tag size is determined as 76 bps INFO @ Tue, 30 Jun 2020 01:46:27: #1 tag size = 76 INFO @ Tue, 30 Jun 2020 01:46:27: #1 total tags in treatment: 16323915 INFO @ Tue, 30 Jun 2020 01:46:27: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:46:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) BigWig に変換しました。 /var/spool/uge/it011/job_scripts/6529210: line 293: 24400 Terminated MACS $i /var/spool/uge/it011/job_scripts/6529210: line 293: 31727 Terminated MACS $i /var/spool/uge/it011/job_scripts/6529210: line 293: 32022 Terminated MACS $i ls: cannot access SRX042246.05.bed: No such file or directory mv: cannot stat ‘SRX042246.05.bed’: No such file or directory mv: cannot stat ‘SRX042246.05.bb’: No such file or directory ls: cannot access SRX042246.10.bed: No such file or directory mv: cannot stat ‘SRX042246.10.bed’: No such file or directory mv: cannot stat ‘SRX042246.10.bb’: No such file or directory ls: cannot access SRX042246.20.bed: No such file or directory mv: cannot stat ‘SRX042246.20.bed’: No such file or directory mv: cannot stat ‘SRX042246.20.bb’: No such file or directory