Job ID = 6452896 SRX = SRX041397 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:08:03 prefetch.2.10.7: 1) Downloading 'SRR099132'... 2020-06-21T08:08:03 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:10:13 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:10:14 prefetch.2.10.7: 'SRR099132' is valid 2020-06-21T08:10:14 prefetch.2.10.7: 1) 'SRR099132' was downloaded successfully Read 18604075 spots for SRR099132/SRR099132.sra Written 18604075 spots for SRR099132/SRR099132.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:41 18604075 reads; of these: 18604075 (100.00%) were unpaired; of these: 758227 (4.08%) aligned 0 times 14672233 (78.87%) aligned exactly 1 time 3173615 (17.06%) aligned >1 times 95.92% overall alignment rate Time searching: 00:03:41 Overall time: 00:03:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2048525 / 17845848 = 0.1148 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:20:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX041397/SRX041397.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX041397/SRX041397.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX041397/SRX041397.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX041397/SRX041397.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:20:25: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:20:25: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:20:32: 1000000 INFO @ Sun, 21 Jun 2020 17:20:39: 2000000 INFO @ Sun, 21 Jun 2020 17:20:47: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:20:54: 4000000 INFO @ Sun, 21 Jun 2020 17:20:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX041397/SRX041397.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX041397/SRX041397.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX041397/SRX041397.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX041397/SRX041397.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:20:55: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:20:55: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:21:02: 5000000 INFO @ Sun, 21 Jun 2020 17:21:02: 1000000 INFO @ Sun, 21 Jun 2020 17:21:09: 6000000 INFO @ Sun, 21 Jun 2020 17:21:10: 2000000 INFO @ Sun, 21 Jun 2020 17:21:16: 7000000 INFO @ Sun, 21 Jun 2020 17:21:17: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:21:23: 8000000 INFO @ Sun, 21 Jun 2020 17:21:24: 4000000 INFO @ Sun, 21 Jun 2020 17:21:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX041397/SRX041397.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX041397/SRX041397.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX041397/SRX041397.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX041397/SRX041397.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:21:25: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:21:25: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:21:31: 9000000 INFO @ Sun, 21 Jun 2020 17:21:32: 5000000 INFO @ Sun, 21 Jun 2020 17:21:32: 1000000 INFO @ Sun, 21 Jun 2020 17:21:39: 10000000 INFO @ Sun, 21 Jun 2020 17:21:39: 6000000 INFO @ Sun, 21 Jun 2020 17:21:39: 2000000 INFO @ Sun, 21 Jun 2020 17:21:46: 11000000 INFO @ Sun, 21 Jun 2020 17:21:47: 7000000 INFO @ Sun, 21 Jun 2020 17:21:47: 3000000 INFO @ Sun, 21 Jun 2020 17:21:53: 12000000 INFO @ Sun, 21 Jun 2020 17:21:54: 8000000 INFO @ Sun, 21 Jun 2020 17:21:54: 4000000 INFO @ Sun, 21 Jun 2020 17:22:01: 9000000 INFO @ Sun, 21 Jun 2020 17:22:01: 13000000 INFO @ Sun, 21 Jun 2020 17:22:02: 5000000 INFO @ Sun, 21 Jun 2020 17:22:09: 10000000 INFO @ Sun, 21 Jun 2020 17:22:09: 14000000 INFO @ Sun, 21 Jun 2020 17:22:10: 6000000 INFO @ Sun, 21 Jun 2020 17:22:16: 11000000 INFO @ Sun, 21 Jun 2020 17:22:16: 15000000 INFO @ Sun, 21 Jun 2020 17:22:17: 7000000 INFO @ Sun, 21 Jun 2020 17:22:22: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 17:22:22: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 17:22:22: #1 total tags in treatment: 15797323 INFO @ Sun, 21 Jun 2020 17:22:22: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:22:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:22:23: 12000000 INFO @ Sun, 21 Jun 2020 17:22:23: #1 tags after filtering in treatment: 15797312 INFO @ Sun, 21 Jun 2020 17:22:23: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:22:23: #1 finished! INFO @ Sun, 21 Jun 2020 17:22:23: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:22:23: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:22:24: #2 number of paired peaks: 74 WARNING @ Sun, 21 Jun 2020 17:22:24: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:22:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX041397/SRX041397.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX041397/SRX041397.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX041397/SRX041397.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX041397/SRX041397.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:22:25: 8000000 INFO @ Sun, 21 Jun 2020 17:22:31: 13000000 INFO @ Sun, 21 Jun 2020 17:22:32: 9000000 INFO @ Sun, 21 Jun 2020 17:22:38: 14000000 INFO @ Sun, 21 Jun 2020 17:22:39: 10000000 INFO @ Sun, 21 Jun 2020 17:22:45: 15000000 INFO @ Sun, 21 Jun 2020 17:22:46: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:22:51: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 17:22:51: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 17:22:51: #1 total tags in treatment: 15797323 INFO @ Sun, 21 Jun 2020 17:22:51: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:22:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:22:52: #1 tags after filtering in treatment: 15797312 INFO @ Sun, 21 Jun 2020 17:22:52: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:22:52: #1 finished! INFO @ Sun, 21 Jun 2020 17:22:52: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:22:52: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:22:53: #2 number of paired peaks: 74 WARNING @ Sun, 21 Jun 2020 17:22:53: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:22:53: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 17:22:53: 12000000 cut: /home/okishinya/chipatlas/results/dm6/SRX041397/SRX041397.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX041397/SRX041397.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX041397/SRX041397.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX041397/SRX041397.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:23:00: 13000000 INFO @ Sun, 21 Jun 2020 17:23:07: 14000000 INFO @ Sun, 21 Jun 2020 17:23:14: 15000000 INFO @ Sun, 21 Jun 2020 17:23:20: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 17:23:20: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 17:23:20: #1 total tags in treatment: 15797323 INFO @ Sun, 21 Jun 2020 17:23:20: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:23:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:23:21: #1 tags after filtering in treatment: 15797312 INFO @ Sun, 21 Jun 2020 17:23:21: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:23:21: #1 finished! INFO @ Sun, 21 Jun 2020 17:23:21: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:23:21: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:23:22: #2 number of paired peaks: 74 WARNING @ Sun, 21 Jun 2020 17:23:22: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:23:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX041397/SRX041397.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX041397/SRX041397.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX041397/SRX041397.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX041397/SRX041397.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。