Job ID = 6452823 SRX = SRX041383 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:14:03 prefetch.2.10.7: 1) Downloading 'SRR099111'... 2020-06-21T08:14:03 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:16:36 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:16:36 prefetch.2.10.7: 1) 'SRR099111' was downloaded successfully Read 28434966 spots for SRR099111/SRR099111.sra Written 28434966 spots for SRR099111/SRR099111.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:44 28434966 reads; of these: 28434966 (100.00%) were unpaired; of these: 1566676 (5.51%) aligned 0 times 21989736 (77.33%) aligned exactly 1 time 4878554 (17.16%) aligned >1 times 94.49% overall alignment rate Time searching: 00:05:44 Overall time: 00:05:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4734298 / 26868290 = 0.1762 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:29:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX041383/SRX041383.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX041383/SRX041383.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX041383/SRX041383.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX041383/SRX041383.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:29:10: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:29:10: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:29:15: 1000000 INFO @ Sun, 21 Jun 2020 17:29:20: 2000000 INFO @ Sun, 21 Jun 2020 17:29:25: 3000000 INFO @ Sun, 21 Jun 2020 17:29:30: 4000000 INFO @ Sun, 21 Jun 2020 17:29:34: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:29:39: 6000000 INFO @ Sun, 21 Jun 2020 17:29:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX041383/SRX041383.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX041383/SRX041383.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX041383/SRX041383.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX041383/SRX041383.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:29:40: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:29:40: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:29:44: 7000000 INFO @ Sun, 21 Jun 2020 17:29:45: 1000000 INFO @ Sun, 21 Jun 2020 17:29:49: 8000000 INFO @ Sun, 21 Jun 2020 17:29:50: 2000000 INFO @ Sun, 21 Jun 2020 17:29:54: 9000000 INFO @ Sun, 21 Jun 2020 17:29:55: 3000000 INFO @ Sun, 21 Jun 2020 17:29:59: 10000000 INFO @ Sun, 21 Jun 2020 17:30:00: 4000000 INFO @ Sun, 21 Jun 2020 17:30:04: 11000000 INFO @ Sun, 21 Jun 2020 17:30:05: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:30:09: 12000000 INFO @ Sun, 21 Jun 2020 17:30:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX041383/SRX041383.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX041383/SRX041383.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX041383/SRX041383.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX041383/SRX041383.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:30:10: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:30:10: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:30:10: 6000000 INFO @ Sun, 21 Jun 2020 17:30:14: 13000000 INFO @ Sun, 21 Jun 2020 17:30:15: 1000000 INFO @ Sun, 21 Jun 2020 17:30:15: 7000000 INFO @ Sun, 21 Jun 2020 17:30:19: 14000000 INFO @ Sun, 21 Jun 2020 17:30:20: 2000000 INFO @ Sun, 21 Jun 2020 17:30:20: 8000000 INFO @ Sun, 21 Jun 2020 17:30:24: 15000000 INFO @ Sun, 21 Jun 2020 17:30:25: 3000000 INFO @ Sun, 21 Jun 2020 17:30:26: 9000000 INFO @ Sun, 21 Jun 2020 17:30:29: 16000000 INFO @ Sun, 21 Jun 2020 17:30:30: 4000000 INFO @ Sun, 21 Jun 2020 17:30:31: 10000000 INFO @ Sun, 21 Jun 2020 17:30:34: 17000000 INFO @ Sun, 21 Jun 2020 17:30:36: 5000000 INFO @ Sun, 21 Jun 2020 17:30:36: 11000000 INFO @ Sun, 21 Jun 2020 17:30:40: 18000000 INFO @ Sun, 21 Jun 2020 17:30:41: 6000000 INFO @ Sun, 21 Jun 2020 17:30:41: 12000000 INFO @ Sun, 21 Jun 2020 17:30:45: 19000000 INFO @ Sun, 21 Jun 2020 17:30:47: 7000000 INFO @ Sun, 21 Jun 2020 17:30:47: 13000000 INFO @ Sun, 21 Jun 2020 17:30:50: 20000000 INFO @ Sun, 21 Jun 2020 17:30:52: 8000000 INFO @ Sun, 21 Jun 2020 17:30:53: 14000000 INFO @ Sun, 21 Jun 2020 17:30:56: 21000000 INFO @ Sun, 21 Jun 2020 17:30:58: 9000000 INFO @ Sun, 21 Jun 2020 17:30:58: 15000000 INFO @ Sun, 21 Jun 2020 17:31:01: 22000000 INFO @ Sun, 21 Jun 2020 17:31:02: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 17:31:02: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 17:31:02: #1 total tags in treatment: 22133992 INFO @ Sun, 21 Jun 2020 17:31:02: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:31:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:31:02: #1 tags after filtering in treatment: 22133939 INFO @ Sun, 21 Jun 2020 17:31:02: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:31:02: #1 finished! INFO @ Sun, 21 Jun 2020 17:31:02: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:31:02: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:31:03: 10000000 INFO @ Sun, 21 Jun 2020 17:31:04: 16000000 INFO @ Sun, 21 Jun 2020 17:31:04: #2 number of paired peaks: 86 WARNING @ Sun, 21 Jun 2020 17:31:04: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:31:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX041383/SRX041383.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX041383/SRX041383.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX041383/SRX041383.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX041383/SRX041383.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:31:08: 11000000 INFO @ Sun, 21 Jun 2020 17:31:09: 17000000 INFO @ Sun, 21 Jun 2020 17:31:13: 12000000 INFO @ Sun, 21 Jun 2020 17:31:14: 18000000 INFO @ Sun, 21 Jun 2020 17:31:18: 13000000 INFO @ Sun, 21 Jun 2020 17:31:19: 19000000 INFO @ Sun, 21 Jun 2020 17:31:23: 14000000 INFO @ Sun, 21 Jun 2020 17:31:24: 20000000 INFO @ Sun, 21 Jun 2020 17:31:28: 15000000 INFO @ Sun, 21 Jun 2020 17:31:30: 21000000 INFO @ Sun, 21 Jun 2020 17:31:33: 16000000 INFO @ Sun, 21 Jun 2020 17:31:35: 22000000 INFO @ Sun, 21 Jun 2020 17:31:36: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 17:31:36: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 17:31:36: #1 total tags in treatment: 22133992 INFO @ Sun, 21 Jun 2020 17:31:36: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:31:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:31:37: #1 tags after filtering in treatment: 22133939 INFO @ Sun, 21 Jun 2020 17:31:37: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:31:37: #1 finished! INFO @ Sun, 21 Jun 2020 17:31:37: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:31:37: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:31:38: #2 number of paired peaks: 86 WARNING @ Sun, 21 Jun 2020 17:31:38: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:31:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX041383/SRX041383.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX041383/SRX041383.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX041383/SRX041383.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX041383/SRX041383.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:31:39: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:31:44: 18000000 INFO @ Sun, 21 Jun 2020 17:31:49: 19000000 INFO @ Sun, 21 Jun 2020 17:31:54: 20000000 INFO @ Sun, 21 Jun 2020 17:31:59: 21000000 INFO @ Sun, 21 Jun 2020 17:32:04: 22000000 INFO @ Sun, 21 Jun 2020 17:32:05: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 17:32:05: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 17:32:05: #1 total tags in treatment: 22133992 INFO @ Sun, 21 Jun 2020 17:32:05: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:32:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:32:06: #1 tags after filtering in treatment: 22133939 INFO @ Sun, 21 Jun 2020 17:32:06: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:32:06: #1 finished! INFO @ Sun, 21 Jun 2020 17:32:06: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:32:06: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:32:07: #2 number of paired peaks: 86 WARNING @ Sun, 21 Jun 2020 17:32:07: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:32:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX041383/SRX041383.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX041383/SRX041383.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX041383/SRX041383.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX041383/SRX041383.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。