Job ID = 6452804 SRX = SRX033323 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T07:43:36 prefetch.2.10.7: 1) Downloading 'SRR080719'... 2020-06-21T07:43:36 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T07:47:37 prefetch.2.10.7: HTTPS download succeed 2020-06-21T07:47:37 prefetch.2.10.7: 'SRR080719' is valid 2020-06-21T07:47:37 prefetch.2.10.7: 1) 'SRR080719' was downloaded successfully Read 16096991 spots for SRR080719/SRR080719.sra Written 16096991 spots for SRR080719/SRR080719.sra 2020-06-21T07:48:33 prefetch.2.10.7: 1) Downloading 'SRR080720'... 2020-06-21T07:48:33 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T07:53:28 prefetch.2.10.7: HTTPS download succeed 2020-06-21T07:53:28 prefetch.2.10.7: 'SRR080720' is valid 2020-06-21T07:53:28 prefetch.2.10.7: 1) 'SRR080720' was downloaded successfully Read 14795589 spots for SRR080720/SRR080720.sra Written 14795589 spots for SRR080720/SRR080720.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:52 30892580 reads; of these: 30892580 (100.00%) were unpaired; of these: 1668759 (5.40%) aligned 0 times 24303883 (78.67%) aligned exactly 1 time 4919938 (15.93%) aligned >1 times 94.60% overall alignment rate Time searching: 00:05:52 Overall time: 00:05:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7188162 / 29223821 = 0.2460 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:06:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX033323/SRX033323.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX033323/SRX033323.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX033323/SRX033323.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX033323/SRX033323.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:06:17: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:06:17: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:06:22: 1000000 INFO @ Sun, 21 Jun 2020 17:06:27: 2000000 INFO @ Sun, 21 Jun 2020 17:06:31: 3000000 INFO @ Sun, 21 Jun 2020 17:06:36: 4000000 INFO @ Sun, 21 Jun 2020 17:06:41: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:06:46: 6000000 INFO @ Sun, 21 Jun 2020 17:06:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX033323/SRX033323.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX033323/SRX033323.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX033323/SRX033323.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX033323/SRX033323.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:06:47: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:06:47: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:06:52: 7000000 INFO @ Sun, 21 Jun 2020 17:06:52: 1000000 INFO @ Sun, 21 Jun 2020 17:06:57: 8000000 INFO @ Sun, 21 Jun 2020 17:06:57: 2000000 INFO @ Sun, 21 Jun 2020 17:07:02: 9000000 INFO @ Sun, 21 Jun 2020 17:07:02: 3000000 INFO @ Sun, 21 Jun 2020 17:07:07: 4000000 INFO @ Sun, 21 Jun 2020 17:07:07: 10000000 INFO @ Sun, 21 Jun 2020 17:07:12: 5000000 INFO @ Sun, 21 Jun 2020 17:07:12: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:07:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX033323/SRX033323.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX033323/SRX033323.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX033323/SRX033323.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX033323/SRX033323.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:07:17: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:07:17: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:07:17: 12000000 INFO @ Sun, 21 Jun 2020 17:07:17: 6000000 INFO @ Sun, 21 Jun 2020 17:07:22: 1000000 INFO @ Sun, 21 Jun 2020 17:07:22: 7000000 INFO @ Sun, 21 Jun 2020 17:07:23: 13000000 INFO @ Sun, 21 Jun 2020 17:07:27: 2000000 INFO @ Sun, 21 Jun 2020 17:07:28: 8000000 INFO @ Sun, 21 Jun 2020 17:07:28: 14000000 INFO @ Sun, 21 Jun 2020 17:07:33: 3000000 INFO @ Sun, 21 Jun 2020 17:07:33: 9000000 INFO @ Sun, 21 Jun 2020 17:07:33: 15000000 INFO @ Sun, 21 Jun 2020 17:07:38: 10000000 INFO @ Sun, 21 Jun 2020 17:07:38: 4000000 INFO @ Sun, 21 Jun 2020 17:07:38: 16000000 INFO @ Sun, 21 Jun 2020 17:07:43: 11000000 INFO @ Sun, 21 Jun 2020 17:07:43: 5000000 INFO @ Sun, 21 Jun 2020 17:07:44: 17000000 INFO @ Sun, 21 Jun 2020 17:07:48: 12000000 INFO @ Sun, 21 Jun 2020 17:07:48: 6000000 INFO @ Sun, 21 Jun 2020 17:07:49: 18000000 INFO @ Sun, 21 Jun 2020 17:07:53: 13000000 INFO @ Sun, 21 Jun 2020 17:07:54: 7000000 INFO @ Sun, 21 Jun 2020 17:07:55: 19000000 INFO @ Sun, 21 Jun 2020 17:07:59: 14000000 INFO @ Sun, 21 Jun 2020 17:07:59: 8000000 INFO @ Sun, 21 Jun 2020 17:08:00: 20000000 INFO @ Sun, 21 Jun 2020 17:08:04: 15000000 INFO @ Sun, 21 Jun 2020 17:08:04: 9000000 INFO @ Sun, 21 Jun 2020 17:08:06: 21000000 INFO @ Sun, 21 Jun 2020 17:08:09: 16000000 INFO @ Sun, 21 Jun 2020 17:08:09: 10000000 INFO @ Sun, 21 Jun 2020 17:08:11: 22000000 INFO @ Sun, 21 Jun 2020 17:08:12: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 17:08:12: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 17:08:12: #1 total tags in treatment: 22035659 INFO @ Sun, 21 Jun 2020 17:08:12: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:08:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:08:12: #1 tags after filtering in treatment: 22035657 INFO @ Sun, 21 Jun 2020 17:08:12: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:08:12: #1 finished! INFO @ Sun, 21 Jun 2020 17:08:12: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:08:12: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:08:14: #2 number of paired peaks: 64 WARNING @ Sun, 21 Jun 2020 17:08:14: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:08:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX033323/SRX033323.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX033323/SRX033323.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX033323/SRX033323.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX033323/SRX033323.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:08:14: 17000000 INFO @ Sun, 21 Jun 2020 17:08:15: 11000000 INFO @ Sun, 21 Jun 2020 17:08:19: 18000000 INFO @ Sun, 21 Jun 2020 17:08:20: 12000000 INFO @ Sun, 21 Jun 2020 17:08:25: 19000000 INFO @ Sun, 21 Jun 2020 17:08:25: 13000000 INFO @ Sun, 21 Jun 2020 17:08:30: 20000000 INFO @ Sun, 21 Jun 2020 17:08:31: 14000000 INFO @ Sun, 21 Jun 2020 17:08:35: 21000000 INFO @ Sun, 21 Jun 2020 17:08:36: 15000000 INFO @ Sun, 21 Jun 2020 17:08:40: 22000000 INFO @ Sun, 21 Jun 2020 17:08:41: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 17:08:41: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 17:08:41: #1 total tags in treatment: 22035659 INFO @ Sun, 21 Jun 2020 17:08:41: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:08:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:08:41: #1 tags after filtering in treatment: 22035657 INFO @ Sun, 21 Jun 2020 17:08:41: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:08:41: #1 finished! INFO @ Sun, 21 Jun 2020 17:08:41: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:08:41: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:08:41: 16000000 INFO @ Sun, 21 Jun 2020 17:08:43: #2 number of paired peaks: 64 WARNING @ Sun, 21 Jun 2020 17:08:43: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:08:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX033323/SRX033323.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX033323/SRX033323.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX033323/SRX033323.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX033323/SRX033323.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:08:47: 17000000 INFO @ Sun, 21 Jun 2020 17:08:52: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:08:58: 19000000 INFO @ Sun, 21 Jun 2020 17:09:03: 20000000 INFO @ Sun, 21 Jun 2020 17:09:08: 21000000 INFO @ Sun, 21 Jun 2020 17:09:14: 22000000 INFO @ Sun, 21 Jun 2020 17:09:14: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 17:09:14: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 17:09:14: #1 total tags in treatment: 22035659 INFO @ Sun, 21 Jun 2020 17:09:14: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:09:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:09:15: #1 tags after filtering in treatment: 22035657 INFO @ Sun, 21 Jun 2020 17:09:15: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:09:15: #1 finished! INFO @ Sun, 21 Jun 2020 17:09:15: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:09:15: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:09:16: #2 number of paired peaks: 64 WARNING @ Sun, 21 Jun 2020 17:09:16: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:09:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX033323/SRX033323.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX033323/SRX033323.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX033323/SRX033323.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX033323/SRX033323.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。