Job ID = 6452790 SRX = SRX033313 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T07:43:06 prefetch.2.10.7: 1) Downloading 'SRR080705'... 2020-06-21T07:43:06 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T07:46:00 prefetch.2.10.7: HTTPS download succeed 2020-06-21T07:46:01 prefetch.2.10.7: 'SRR080705' is valid 2020-06-21T07:46:01 prefetch.2.10.7: 1) 'SRR080705' was downloaded successfully Read 25675302 spots for SRR080705/SRR080705.sra Written 25675302 spots for SRR080705/SRR080705.sra 2020-06-21T07:47:17 prefetch.2.10.7: 1) Downloading 'SRR080706'... 2020-06-21T07:47:17 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T07:50:37 prefetch.2.10.7: HTTPS download succeed 2020-06-21T07:50:37 prefetch.2.10.7: 1) 'SRR080706' was downloaded successfully Read 29456758 spots for SRR080706/SRR080706.sra Written 29456758 spots for SRR080706/SRR080706.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:50 55132060 reads; of these: 55132060 (100.00%) were unpaired; of these: 2438319 (4.42%) aligned 0 times 46591053 (84.51%) aligned exactly 1 time 6102688 (11.07%) aligned >1 times 95.58% overall alignment rate Time searching: 00:09:50 Overall time: 00:09:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 14277454 / 52693741 = 0.2710 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:11:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX033313/SRX033313.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX033313/SRX033313.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX033313/SRX033313.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX033313/SRX033313.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:11:29: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:11:29: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:11:33: 1000000 INFO @ Sun, 21 Jun 2020 17:11:37: 2000000 INFO @ Sun, 21 Jun 2020 17:11:42: 3000000 INFO @ Sun, 21 Jun 2020 17:11:46: 4000000 INFO @ Sun, 21 Jun 2020 17:11:50: 5000000 INFO @ Sun, 21 Jun 2020 17:11:54: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:11:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX033313/SRX033313.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX033313/SRX033313.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX033313/SRX033313.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX033313/SRX033313.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:11:58: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:11:58: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:11:59: 7000000 INFO @ Sun, 21 Jun 2020 17:12:03: 1000000 INFO @ Sun, 21 Jun 2020 17:12:03: 8000000 INFO @ Sun, 21 Jun 2020 17:12:07: 2000000 INFO @ Sun, 21 Jun 2020 17:12:07: 9000000 INFO @ Sun, 21 Jun 2020 17:12:11: 3000000 INFO @ Sun, 21 Jun 2020 17:12:12: 10000000 INFO @ Sun, 21 Jun 2020 17:12:15: 4000000 INFO @ Sun, 21 Jun 2020 17:12:16: 11000000 INFO @ Sun, 21 Jun 2020 17:12:20: 5000000 INFO @ Sun, 21 Jun 2020 17:12:20: 12000000 INFO @ Sun, 21 Jun 2020 17:12:24: 6000000 INFO @ Sun, 21 Jun 2020 17:12:25: 13000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:12:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX033313/SRX033313.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX033313/SRX033313.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX033313/SRX033313.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX033313/SRX033313.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:12:28: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:12:28: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:12:28: 7000000 INFO @ Sun, 21 Jun 2020 17:12:29: 14000000 INFO @ Sun, 21 Jun 2020 17:12:33: 1000000 INFO @ Sun, 21 Jun 2020 17:12:33: 8000000 INFO @ Sun, 21 Jun 2020 17:12:33: 15000000 INFO @ Sun, 21 Jun 2020 17:12:37: 2000000 INFO @ Sun, 21 Jun 2020 17:12:37: 9000000 INFO @ Sun, 21 Jun 2020 17:12:38: 16000000 INFO @ Sun, 21 Jun 2020 17:12:41: 3000000 INFO @ Sun, 21 Jun 2020 17:12:41: 10000000 INFO @ Sun, 21 Jun 2020 17:12:42: 17000000 INFO @ Sun, 21 Jun 2020 17:12:46: 4000000 INFO @ Sun, 21 Jun 2020 17:12:46: 11000000 INFO @ Sun, 21 Jun 2020 17:12:46: 18000000 INFO @ Sun, 21 Jun 2020 17:12:50: 5000000 INFO @ Sun, 21 Jun 2020 17:12:50: 12000000 INFO @ Sun, 21 Jun 2020 17:12:51: 19000000 INFO @ Sun, 21 Jun 2020 17:12:54: 6000000 INFO @ Sun, 21 Jun 2020 17:12:54: 13000000 INFO @ Sun, 21 Jun 2020 17:12:55: 20000000 INFO @ Sun, 21 Jun 2020 17:12:58: 7000000 INFO @ Sun, 21 Jun 2020 17:12:59: 14000000 INFO @ Sun, 21 Jun 2020 17:12:59: 21000000 INFO @ Sun, 21 Jun 2020 17:13:03: 8000000 INFO @ Sun, 21 Jun 2020 17:13:03: 15000000 INFO @ Sun, 21 Jun 2020 17:13:03: 22000000 INFO @ Sun, 21 Jun 2020 17:13:07: 9000000 INFO @ Sun, 21 Jun 2020 17:13:07: 16000000 INFO @ Sun, 21 Jun 2020 17:13:08: 23000000 INFO @ Sun, 21 Jun 2020 17:13:11: 17000000 INFO @ Sun, 21 Jun 2020 17:13:11: 10000000 INFO @ Sun, 21 Jun 2020 17:13:12: 24000000 INFO @ Sun, 21 Jun 2020 17:13:16: 18000000 INFO @ Sun, 21 Jun 2020 17:13:16: 11000000 INFO @ Sun, 21 Jun 2020 17:13:16: 25000000 INFO @ Sun, 21 Jun 2020 17:13:20: 19000000 INFO @ Sun, 21 Jun 2020 17:13:20: 12000000 INFO @ Sun, 21 Jun 2020 17:13:21: 26000000 INFO @ Sun, 21 Jun 2020 17:13:24: 20000000 INFO @ Sun, 21 Jun 2020 17:13:25: 13000000 INFO @ Sun, 21 Jun 2020 17:13:25: 27000000 INFO @ Sun, 21 Jun 2020 17:13:29: 21000000 INFO @ Sun, 21 Jun 2020 17:13:29: 14000000 INFO @ Sun, 21 Jun 2020 17:13:29: 28000000 INFO @ Sun, 21 Jun 2020 17:13:33: 22000000 INFO @ Sun, 21 Jun 2020 17:13:33: 15000000 INFO @ Sun, 21 Jun 2020 17:13:33: 29000000 INFO @ Sun, 21 Jun 2020 17:13:37: 23000000 INFO @ Sun, 21 Jun 2020 17:13:37: 16000000 INFO @ Sun, 21 Jun 2020 17:13:38: 30000000 INFO @ Sun, 21 Jun 2020 17:13:41: 24000000 INFO @ Sun, 21 Jun 2020 17:13:42: 17000000 INFO @ Sun, 21 Jun 2020 17:13:42: 31000000 INFO @ Sun, 21 Jun 2020 17:13:45: 25000000 INFO @ Sun, 21 Jun 2020 17:13:46: 18000000 INFO @ Sun, 21 Jun 2020 17:13:46: 32000000 INFO @ Sun, 21 Jun 2020 17:13:50: 26000000 INFO @ Sun, 21 Jun 2020 17:13:50: 19000000 INFO @ Sun, 21 Jun 2020 17:13:51: 33000000 INFO @ Sun, 21 Jun 2020 17:13:54: 27000000 INFO @ Sun, 21 Jun 2020 17:13:55: 20000000 INFO @ Sun, 21 Jun 2020 17:13:55: 34000000 INFO @ Sun, 21 Jun 2020 17:13:58: 28000000 INFO @ Sun, 21 Jun 2020 17:13:59: 21000000 INFO @ Sun, 21 Jun 2020 17:13:59: 35000000 INFO @ Sun, 21 Jun 2020 17:14:02: 29000000 INFO @ Sun, 21 Jun 2020 17:14:03: 22000000 INFO @ Sun, 21 Jun 2020 17:14:04: 36000000 INFO @ Sun, 21 Jun 2020 17:14:06: 30000000 INFO @ Sun, 21 Jun 2020 17:14:07: 23000000 INFO @ Sun, 21 Jun 2020 17:14:08: 37000000 INFO @ Sun, 21 Jun 2020 17:14:11: 31000000 INFO @ Sun, 21 Jun 2020 17:14:11: 24000000 INFO @ Sun, 21 Jun 2020 17:14:12: 38000000 INFO @ Sun, 21 Jun 2020 17:14:14: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 17:14:14: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 17:14:14: #1 total tags in treatment: 38416287 INFO @ Sun, 21 Jun 2020 17:14:14: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:14:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:14:15: #1 tags after filtering in treatment: 38416286 INFO @ Sun, 21 Jun 2020 17:14:15: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:14:15: #1 finished! INFO @ Sun, 21 Jun 2020 17:14:15: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:14:15: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:14:15: 32000000 INFO @ Sun, 21 Jun 2020 17:14:16: 25000000 INFO @ Sun, 21 Jun 2020 17:14:18: #2 number of paired peaks: 20 WARNING @ Sun, 21 Jun 2020 17:14:18: Too few paired peaks (20) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:14:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX033313/SRX033313.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX033313/SRX033313.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX033313/SRX033313.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX033313/SRX033313.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:14:20: 33000000 INFO @ Sun, 21 Jun 2020 17:14:20: 26000000 INFO @ Sun, 21 Jun 2020 17:14:24: 34000000 INFO @ Sun, 21 Jun 2020 17:14:24: 27000000 INFO @ Sun, 21 Jun 2020 17:14:28: 35000000 INFO @ Sun, 21 Jun 2020 17:14:28: 28000000 INFO @ Sun, 21 Jun 2020 17:14:32: 36000000 INFO @ Sun, 21 Jun 2020 17:14:32: 29000000 INFO @ Sun, 21 Jun 2020 17:14:36: 37000000 INFO @ Sun, 21 Jun 2020 17:14:37: 30000000 INFO @ Sun, 21 Jun 2020 17:14:41: 38000000 INFO @ Sun, 21 Jun 2020 17:14:41: 31000000 INFO @ Sun, 21 Jun 2020 17:14:43: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 17:14:43: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 17:14:43: #1 total tags in treatment: 38416287 INFO @ Sun, 21 Jun 2020 17:14:43: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:14:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:14:44: #1 tags after filtering in treatment: 38416286 INFO @ Sun, 21 Jun 2020 17:14:44: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:14:44: #1 finished! INFO @ Sun, 21 Jun 2020 17:14:44: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:14:44: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:14:46: 32000000 INFO @ Sun, 21 Jun 2020 17:14:46: #2 number of paired peaks: 20 WARNING @ Sun, 21 Jun 2020 17:14:46: Too few paired peaks (20) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:14:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX033313/SRX033313.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX033313/SRX033313.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX033313/SRX033313.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX033313/SRX033313.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:14:50: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:14:54: 34000000 INFO @ Sun, 21 Jun 2020 17:14:58: 35000000 INFO @ Sun, 21 Jun 2020 17:15:02: 36000000 INFO @ Sun, 21 Jun 2020 17:15:06: 37000000 INFO @ Sun, 21 Jun 2020 17:15:11: 38000000 INFO @ Sun, 21 Jun 2020 17:15:13: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 17:15:13: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 17:15:13: #1 total tags in treatment: 38416287 INFO @ Sun, 21 Jun 2020 17:15:13: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:15:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:15:14: #1 tags after filtering in treatment: 38416286 INFO @ Sun, 21 Jun 2020 17:15:14: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:15:14: #1 finished! INFO @ Sun, 21 Jun 2020 17:15:14: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:15:14: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:15:16: #2 number of paired peaks: 20 WARNING @ Sun, 21 Jun 2020 17:15:16: Too few paired peaks (20) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:15:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX033313/SRX033313.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX033313/SRX033313.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX033313/SRX033313.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX033313/SRX033313.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。