Job ID = 6529187 SRX = SRX032127 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:29 15825369 reads; of these: 15825369 (100.00%) were unpaired; of these: 1931920 (12.21%) aligned 0 times 11870129 (75.01%) aligned exactly 1 time 2023320 (12.79%) aligned >1 times 87.79% overall alignment rate Time searching: 00:02:29 Overall time: 00:02:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 968851 / 13893449 = 0.0697 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:32:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX032127/SRX032127.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX032127/SRX032127.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX032127/SRX032127.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX032127/SRX032127.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:32:57: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:32:57: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:33:03: 1000000 INFO @ Tue, 30 Jun 2020 01:33:09: 2000000 INFO @ Tue, 30 Jun 2020 01:33:15: 3000000 INFO @ Tue, 30 Jun 2020 01:33:21: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:33:27: 5000000 INFO @ Tue, 30 Jun 2020 01:33:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX032127/SRX032127.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX032127/SRX032127.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX032127/SRX032127.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX032127/SRX032127.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:33:27: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:33:27: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:33:33: 6000000 INFO @ Tue, 30 Jun 2020 01:33:33: 1000000 INFO @ Tue, 30 Jun 2020 01:33:39: 7000000 INFO @ Tue, 30 Jun 2020 01:33:39: 2000000 INFO @ Tue, 30 Jun 2020 01:33:45: 8000000 INFO @ Tue, 30 Jun 2020 01:33:45: 3000000 INFO @ Tue, 30 Jun 2020 01:33:51: 9000000 INFO @ Tue, 30 Jun 2020 01:33:51: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:33:57: 10000000 INFO @ Tue, 30 Jun 2020 01:33:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX032127/SRX032127.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX032127/SRX032127.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX032127/SRX032127.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX032127/SRX032127.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:33:57: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:33:57: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:33:57: 5000000 INFO @ Tue, 30 Jun 2020 01:34:03: 1000000 INFO @ Tue, 30 Jun 2020 01:34:04: 11000000 INFO @ Tue, 30 Jun 2020 01:34:04: 6000000 INFO @ Tue, 30 Jun 2020 01:34:10: 2000000 INFO @ Tue, 30 Jun 2020 01:34:10: 12000000 INFO @ Tue, 30 Jun 2020 01:34:10: 7000000 INFO @ Tue, 30 Jun 2020 01:34:16: 3000000 INFO @ Tue, 30 Jun 2020 01:34:16: #1 tag size is determined as 36 bps INFO @ Tue, 30 Jun 2020 01:34:16: #1 tag size = 36 INFO @ Tue, 30 Jun 2020 01:34:16: #1 total tags in treatment: 12924598 INFO @ Tue, 30 Jun 2020 01:34:16: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:34:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:34:17: 8000000 INFO @ Tue, 30 Jun 2020 01:34:17: #1 tags after filtering in treatment: 12924561 INFO @ Tue, 30 Jun 2020 01:34:17: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:34:17: #1 finished! INFO @ Tue, 30 Jun 2020 01:34:17: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:34:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:34:18: #2 number of paired peaks: 46 WARNING @ Tue, 30 Jun 2020 01:34:18: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:34:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX032127/SRX032127.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX032127/SRX032127.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX032127/SRX032127.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX032127/SRX032127.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:34:23: 4000000 INFO @ Tue, 30 Jun 2020 01:34:23: 9000000 INFO @ Tue, 30 Jun 2020 01:34:29: 5000000 INFO @ Tue, 30 Jun 2020 01:34:30: 10000000 INFO @ Tue, 30 Jun 2020 01:34:36: 6000000 INFO @ Tue, 30 Jun 2020 01:34:37: 11000000 INFO @ Tue, 30 Jun 2020 01:34:42: 7000000 INFO @ Tue, 30 Jun 2020 01:34:43: 12000000 INFO @ Tue, 30 Jun 2020 01:34:48: 8000000 INFO @ Tue, 30 Jun 2020 01:34:50: #1 tag size is determined as 36 bps INFO @ Tue, 30 Jun 2020 01:34:50: #1 tag size = 36 INFO @ Tue, 30 Jun 2020 01:34:50: #1 total tags in treatment: 12924598 INFO @ Tue, 30 Jun 2020 01:34:50: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:34:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:34:50: #1 tags after filtering in treatment: 12924561 INFO @ Tue, 30 Jun 2020 01:34:50: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:34:50: #1 finished! INFO @ Tue, 30 Jun 2020 01:34:50: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:34:50: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:34:51: #2 number of paired peaks: 46 WARNING @ Tue, 30 Jun 2020 01:34:51: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:34:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX032127/SRX032127.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX032127/SRX032127.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX032127/SRX032127.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX032127/SRX032127.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 01:34:55: 9000000 INFO @ Tue, 30 Jun 2020 01:35:01: 10000000 INFO @ Tue, 30 Jun 2020 01:35:07: 11000000 INFO @ Tue, 30 Jun 2020 01:35:13: 12000000 INFO @ Tue, 30 Jun 2020 01:35:19: #1 tag size is determined as 36 bps INFO @ Tue, 30 Jun 2020 01:35:19: #1 tag size = 36 INFO @ Tue, 30 Jun 2020 01:35:19: #1 total tags in treatment: 12924598 INFO @ Tue, 30 Jun 2020 01:35:19: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:35:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:35:20: #1 tags after filtering in treatment: 12924561 INFO @ Tue, 30 Jun 2020 01:35:20: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:35:20: #1 finished! INFO @ Tue, 30 Jun 2020 01:35:20: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:35:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:35:21: #2 number of paired peaks: 46 WARNING @ Tue, 30 Jun 2020 01:35:21: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:35:21: Process for pairing-model is terminated! BigWig に変換しました。 cut: /home/okishinya/chipatlas/results/dm6/SRX032127/SRX032127.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX032127/SRX032127.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX032127/SRX032127.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX032127/SRX032127.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling