Job ID = 6452775 SRX = SRX032116 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T07:51:45 prefetch.2.10.7: 1) Downloading 'SRR073936'... 2020-06-21T07:51:45 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T07:53:36 prefetch.2.10.7: HTTPS download succeed 2020-06-21T07:53:37 prefetch.2.10.7: 'SRR073936' is valid 2020-06-21T07:53:37 prefetch.2.10.7: 1) 'SRR073936' was downloaded successfully Read 24471328 spots for SRR073936/SRR073936.sra Written 24471328 spots for SRR073936/SRR073936.sra 2020-06-21T07:54:54 prefetch.2.10.7: 1) Downloading 'SRR073937'... 2020-06-21T07:54:54 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T07:58:32 prefetch.2.10.7: HTTPS download succeed 2020-06-21T07:58:33 prefetch.2.10.7: 'SRR073937' is valid 2020-06-21T07:58:33 prefetch.2.10.7: 1) 'SRR073937' was downloaded successfully Read 22288347 spots for SRR073937/SRR073937.sra Written 22288347 spots for SRR073937/SRR073937.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:28 46759675 reads; of these: 46759675 (100.00%) were unpaired; of these: 1882281 (4.03%) aligned 0 times 33888799 (72.47%) aligned exactly 1 time 10988595 (23.50%) aligned >1 times 95.97% overall alignment rate Time searching: 00:10:28 Overall time: 00:10:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 10175365 / 44877394 = 0.2267 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:19:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX032116/SRX032116.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX032116/SRX032116.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX032116/SRX032116.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX032116/SRX032116.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:19:02: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:19:02: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:19:07: 1000000 INFO @ Sun, 21 Jun 2020 17:19:11: 2000000 INFO @ Sun, 21 Jun 2020 17:19:16: 3000000 INFO @ Sun, 21 Jun 2020 17:19:21: 4000000 INFO @ Sun, 21 Jun 2020 17:19:26: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:19:30: 6000000 INFO @ Sun, 21 Jun 2020 17:19:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX032116/SRX032116.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX032116/SRX032116.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX032116/SRX032116.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX032116/SRX032116.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:19:32: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:19:32: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:19:35: 7000000 INFO @ Sun, 21 Jun 2020 17:19:37: 1000000 INFO @ Sun, 21 Jun 2020 17:19:40: 8000000 INFO @ Sun, 21 Jun 2020 17:19:42: 2000000 INFO @ Sun, 21 Jun 2020 17:19:44: 9000000 INFO @ Sun, 21 Jun 2020 17:19:46: 3000000 INFO @ Sun, 21 Jun 2020 17:19:49: 10000000 INFO @ Sun, 21 Jun 2020 17:19:51: 4000000 INFO @ Sun, 21 Jun 2020 17:19:54: 11000000 INFO @ Sun, 21 Jun 2020 17:19:56: 5000000 INFO @ Sun, 21 Jun 2020 17:19:58: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:20:01: 6000000 INFO @ Sun, 21 Jun 2020 17:20:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX032116/SRX032116.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX032116/SRX032116.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX032116/SRX032116.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX032116/SRX032116.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:20:02: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:20:02: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:20:03: 13000000 INFO @ Sun, 21 Jun 2020 17:20:06: 7000000 INFO @ Sun, 21 Jun 2020 17:20:07: 1000000 INFO @ Sun, 21 Jun 2020 17:20:08: 14000000 INFO @ Sun, 21 Jun 2020 17:20:10: 8000000 INFO @ Sun, 21 Jun 2020 17:20:12: 2000000 INFO @ Sun, 21 Jun 2020 17:20:13: 15000000 INFO @ Sun, 21 Jun 2020 17:20:15: 9000000 INFO @ Sun, 21 Jun 2020 17:20:17: 3000000 INFO @ Sun, 21 Jun 2020 17:20:17: 16000000 INFO @ Sun, 21 Jun 2020 17:20:20: 10000000 INFO @ Sun, 21 Jun 2020 17:20:22: 4000000 INFO @ Sun, 21 Jun 2020 17:20:22: 17000000 INFO @ Sun, 21 Jun 2020 17:20:25: 11000000 INFO @ Sun, 21 Jun 2020 17:20:27: 5000000 INFO @ Sun, 21 Jun 2020 17:20:27: 18000000 INFO @ Sun, 21 Jun 2020 17:20:30: 12000000 INFO @ Sun, 21 Jun 2020 17:20:31: 19000000 INFO @ Sun, 21 Jun 2020 17:20:31: 6000000 INFO @ Sun, 21 Jun 2020 17:20:35: 13000000 INFO @ Sun, 21 Jun 2020 17:20:36: 20000000 INFO @ Sun, 21 Jun 2020 17:20:36: 7000000 INFO @ Sun, 21 Jun 2020 17:20:40: 14000000 INFO @ Sun, 21 Jun 2020 17:20:41: 21000000 INFO @ Sun, 21 Jun 2020 17:20:41: 8000000 INFO @ Sun, 21 Jun 2020 17:20:44: 15000000 INFO @ Sun, 21 Jun 2020 17:20:46: 22000000 INFO @ Sun, 21 Jun 2020 17:20:46: 9000000 INFO @ Sun, 21 Jun 2020 17:20:49: 16000000 INFO @ Sun, 21 Jun 2020 17:20:50: 23000000 INFO @ Sun, 21 Jun 2020 17:20:51: 10000000 INFO @ Sun, 21 Jun 2020 17:20:54: 17000000 INFO @ Sun, 21 Jun 2020 17:20:55: 24000000 INFO @ Sun, 21 Jun 2020 17:20:55: 11000000 INFO @ Sun, 21 Jun 2020 17:20:59: 18000000 INFO @ Sun, 21 Jun 2020 17:21:00: 25000000 INFO @ Sun, 21 Jun 2020 17:21:00: 12000000 INFO @ Sun, 21 Jun 2020 17:21:04: 19000000 INFO @ Sun, 21 Jun 2020 17:21:04: 26000000 INFO @ Sun, 21 Jun 2020 17:21:05: 13000000 INFO @ Sun, 21 Jun 2020 17:21:09: 20000000 INFO @ Sun, 21 Jun 2020 17:21:09: 27000000 INFO @ Sun, 21 Jun 2020 17:21:10: 14000000 INFO @ Sun, 21 Jun 2020 17:21:14: 21000000 INFO @ Sun, 21 Jun 2020 17:21:14: 28000000 INFO @ Sun, 21 Jun 2020 17:21:15: 15000000 INFO @ Sun, 21 Jun 2020 17:21:19: 22000000 INFO @ Sun, 21 Jun 2020 17:21:19: 29000000 INFO @ Sun, 21 Jun 2020 17:21:20: 16000000 INFO @ Sun, 21 Jun 2020 17:21:23: 23000000 INFO @ Sun, 21 Jun 2020 17:21:24: 30000000 INFO @ Sun, 21 Jun 2020 17:21:25: 17000000 INFO @ Sun, 21 Jun 2020 17:21:28: 24000000 INFO @ Sun, 21 Jun 2020 17:21:28: 31000000 INFO @ Sun, 21 Jun 2020 17:21:29: 18000000 INFO @ Sun, 21 Jun 2020 17:21:33: 25000000 INFO @ Sun, 21 Jun 2020 17:21:33: 32000000 INFO @ Sun, 21 Jun 2020 17:21:34: 19000000 INFO @ Sun, 21 Jun 2020 17:21:38: 33000000 INFO @ Sun, 21 Jun 2020 17:21:38: 26000000 INFO @ Sun, 21 Jun 2020 17:21:39: 20000000 INFO @ Sun, 21 Jun 2020 17:21:42: 34000000 INFO @ Sun, 21 Jun 2020 17:21:42: 27000000 INFO @ Sun, 21 Jun 2020 17:21:44: 21000000 INFO @ Sun, 21 Jun 2020 17:21:46: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 17:21:46: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 17:21:46: #1 total tags in treatment: 34702029 INFO @ Sun, 21 Jun 2020 17:21:46: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:21:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:21:47: #1 tags after filtering in treatment: 34702029 INFO @ Sun, 21 Jun 2020 17:21:47: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:21:47: #1 finished! INFO @ Sun, 21 Jun 2020 17:21:47: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:21:47: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:21:47: 28000000 INFO @ Sun, 21 Jun 2020 17:21:49: 22000000 INFO @ Sun, 21 Jun 2020 17:21:49: #2 number of paired peaks: 97 WARNING @ Sun, 21 Jun 2020 17:21:49: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:21:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX032116/SRX032116.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX032116/SRX032116.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX032116/SRX032116.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX032116/SRX032116.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:21:53: 29000000 INFO @ Sun, 21 Jun 2020 17:21:54: 23000000 INFO @ Sun, 21 Jun 2020 17:21:57: 30000000 INFO @ Sun, 21 Jun 2020 17:21:58: 24000000 INFO @ Sun, 21 Jun 2020 17:22:02: 31000000 INFO @ Sun, 21 Jun 2020 17:22:03: 25000000 INFO @ Sun, 21 Jun 2020 17:22:07: 32000000 INFO @ Sun, 21 Jun 2020 17:22:08: 26000000 INFO @ Sun, 21 Jun 2020 17:22:11: 33000000 INFO @ Sun, 21 Jun 2020 17:22:12: 27000000 INFO @ Sun, 21 Jun 2020 17:22:16: 34000000 INFO @ Sun, 21 Jun 2020 17:22:17: 28000000 INFO @ Sun, 21 Jun 2020 17:22:20: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 17:22:20: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 17:22:20: #1 total tags in treatment: 34702029 INFO @ Sun, 21 Jun 2020 17:22:20: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:22:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:22:20: #1 tags after filtering in treatment: 34702029 INFO @ Sun, 21 Jun 2020 17:22:20: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:22:20: #1 finished! INFO @ Sun, 21 Jun 2020 17:22:20: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:22:20: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:22:21: 29000000 INFO @ Sun, 21 Jun 2020 17:22:23: #2 number of paired peaks: 97 WARNING @ Sun, 21 Jun 2020 17:22:23: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:22:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX032116/SRX032116.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX032116/SRX032116.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX032116/SRX032116.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX032116/SRX032116.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:22:26: 30000000 INFO @ Sun, 21 Jun 2020 17:22:30: 31000000 INFO @ Sun, 21 Jun 2020 17:22:35: 32000000 INFO @ Sun, 21 Jun 2020 17:22:39: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:22:43: 34000000 INFO @ Sun, 21 Jun 2020 17:22:47: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 17:22:47: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 17:22:47: #1 total tags in treatment: 34702029 INFO @ Sun, 21 Jun 2020 17:22:47: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:22:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:22:47: #1 tags after filtering in treatment: 34702029 INFO @ Sun, 21 Jun 2020 17:22:47: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:22:47: #1 finished! INFO @ Sun, 21 Jun 2020 17:22:47: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:22:47: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:22:50: #2 number of paired peaks: 97 WARNING @ Sun, 21 Jun 2020 17:22:50: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:22:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX032116/SRX032116.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX032116/SRX032116.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX032116/SRX032116.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX032116/SRX032116.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。