Job ID = 6452756 SRX = SRX030960 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T07:44:36 prefetch.2.10.7: 1) Downloading 'SRR072387'... 2020-06-21T07:44:36 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T07:51:10 prefetch.2.10.7: HTTPS download succeed 2020-06-21T07:51:10 prefetch.2.10.7: 1) 'SRR072387' was downloaded successfully Read 26705535 spots for SRR072387/SRR072387.sra Written 26705535 spots for SRR072387/SRR072387.sra 2020-06-21T07:52:46 prefetch.2.10.7: 1) Downloading 'SRR072388'... 2020-06-21T07:52:46 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T07:54:31 prefetch.2.10.7: HTTPS download succeed 2020-06-21T07:54:32 prefetch.2.10.7: 'SRR072388' is valid 2020-06-21T07:54:32 prefetch.2.10.7: 1) 'SRR072388' was downloaded successfully Read 26973476 spots for SRR072388/SRR072388.sra Written 26973476 spots for SRR072388/SRR072388.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:14 53679011 reads; of these: 53679011 (100.00%) were unpaired; of these: 2590297 (4.83%) aligned 0 times 43581615 (81.19%) aligned exactly 1 time 7507099 (13.99%) aligned >1 times 95.17% overall alignment rate Time searching: 00:10:14 Overall time: 00:10:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 17849058 / 51088714 = 0.3494 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:17:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX030960/SRX030960.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX030960/SRX030960.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX030960/SRX030960.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX030960/SRX030960.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:17:44: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:17:44: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:17:49: 1000000 INFO @ Sun, 21 Jun 2020 17:17:54: 2000000 INFO @ Sun, 21 Jun 2020 17:17:58: 3000000 INFO @ Sun, 21 Jun 2020 17:18:04: 4000000 INFO @ Sun, 21 Jun 2020 17:18:08: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:18:13: 6000000 INFO @ Sun, 21 Jun 2020 17:18:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX030960/SRX030960.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX030960/SRX030960.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX030960/SRX030960.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX030960/SRX030960.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:18:14: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:18:14: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:18:18: 7000000 INFO @ Sun, 21 Jun 2020 17:18:19: 1000000 INFO @ Sun, 21 Jun 2020 17:18:23: 8000000 INFO @ Sun, 21 Jun 2020 17:18:24: 2000000 INFO @ Sun, 21 Jun 2020 17:18:28: 9000000 INFO @ Sun, 21 Jun 2020 17:18:29: 3000000 INFO @ Sun, 21 Jun 2020 17:18:33: 10000000 INFO @ Sun, 21 Jun 2020 17:18:34: 4000000 INFO @ Sun, 21 Jun 2020 17:18:38: 11000000 INFO @ Sun, 21 Jun 2020 17:18:39: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:18:43: 12000000 INFO @ Sun, 21 Jun 2020 17:18:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX030960/SRX030960.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX030960/SRX030960.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX030960/SRX030960.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX030960/SRX030960.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:18:44: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:18:44: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:18:45: 6000000 INFO @ Sun, 21 Jun 2020 17:18:48: 13000000 INFO @ Sun, 21 Jun 2020 17:18:49: 1000000 INFO @ Sun, 21 Jun 2020 17:18:50: 7000000 INFO @ Sun, 21 Jun 2020 17:18:52: 14000000 INFO @ Sun, 21 Jun 2020 17:18:55: 2000000 INFO @ Sun, 21 Jun 2020 17:18:55: 8000000 INFO @ Sun, 21 Jun 2020 17:18:57: 15000000 INFO @ Sun, 21 Jun 2020 17:19:00: 3000000 INFO @ Sun, 21 Jun 2020 17:19:01: 9000000 INFO @ Sun, 21 Jun 2020 17:19:02: 16000000 INFO @ Sun, 21 Jun 2020 17:19:06: 4000000 INFO @ Sun, 21 Jun 2020 17:19:06: 10000000 INFO @ Sun, 21 Jun 2020 17:19:07: 17000000 INFO @ Sun, 21 Jun 2020 17:19:11: 18000000 INFO @ Sun, 21 Jun 2020 17:19:11: 5000000 INFO @ Sun, 21 Jun 2020 17:19:12: 11000000 INFO @ Sun, 21 Jun 2020 17:19:16: 19000000 INFO @ Sun, 21 Jun 2020 17:19:17: 6000000 INFO @ Sun, 21 Jun 2020 17:19:17: 12000000 INFO @ Sun, 21 Jun 2020 17:19:21: 20000000 INFO @ Sun, 21 Jun 2020 17:19:22: 7000000 INFO @ Sun, 21 Jun 2020 17:19:23: 13000000 INFO @ Sun, 21 Jun 2020 17:19:26: 21000000 INFO @ Sun, 21 Jun 2020 17:19:28: 8000000 INFO @ Sun, 21 Jun 2020 17:19:28: 14000000 INFO @ Sun, 21 Jun 2020 17:19:30: 22000000 INFO @ Sun, 21 Jun 2020 17:19:33: 9000000 INFO @ Sun, 21 Jun 2020 17:19:34: 15000000 INFO @ Sun, 21 Jun 2020 17:19:35: 23000000 INFO @ Sun, 21 Jun 2020 17:19:39: 10000000 INFO @ Sun, 21 Jun 2020 17:19:39: 16000000 INFO @ Sun, 21 Jun 2020 17:19:40: 24000000 INFO @ Sun, 21 Jun 2020 17:19:44: 17000000 INFO @ Sun, 21 Jun 2020 17:19:45: 25000000 INFO @ Sun, 21 Jun 2020 17:19:45: 11000000 INFO @ Sun, 21 Jun 2020 17:19:50: 18000000 INFO @ Sun, 21 Jun 2020 17:19:50: 26000000 INFO @ Sun, 21 Jun 2020 17:19:51: 12000000 INFO @ Sun, 21 Jun 2020 17:19:55: 27000000 INFO @ Sun, 21 Jun 2020 17:19:55: 19000000 INFO @ Sun, 21 Jun 2020 17:19:56: 13000000 INFO @ Sun, 21 Jun 2020 17:20:00: 28000000 INFO @ Sun, 21 Jun 2020 17:20:01: 20000000 INFO @ Sun, 21 Jun 2020 17:20:02: 14000000 INFO @ Sun, 21 Jun 2020 17:20:05: 29000000 INFO @ Sun, 21 Jun 2020 17:20:06: 21000000 INFO @ Sun, 21 Jun 2020 17:20:07: 15000000 INFO @ Sun, 21 Jun 2020 17:20:10: 30000000 INFO @ Sun, 21 Jun 2020 17:20:12: 22000000 INFO @ Sun, 21 Jun 2020 17:20:13: 16000000 INFO @ Sun, 21 Jun 2020 17:20:14: 31000000 INFO @ Sun, 21 Jun 2020 17:20:17: 23000000 INFO @ Sun, 21 Jun 2020 17:20:18: 17000000 INFO @ Sun, 21 Jun 2020 17:20:19: 32000000 INFO @ Sun, 21 Jun 2020 17:20:23: 24000000 INFO @ Sun, 21 Jun 2020 17:20:24: 18000000 INFO @ Sun, 21 Jun 2020 17:20:24: 33000000 INFO @ Sun, 21 Jun 2020 17:20:26: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 17:20:26: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 17:20:26: #1 total tags in treatment: 33239656 INFO @ Sun, 21 Jun 2020 17:20:26: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:20:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:20:27: #1 tags after filtering in treatment: 33239656 INFO @ Sun, 21 Jun 2020 17:20:27: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:20:27: #1 finished! INFO @ Sun, 21 Jun 2020 17:20:27: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:20:27: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:20:28: 25000000 INFO @ Sun, 21 Jun 2020 17:20:29: #2 number of paired peaks: 69 WARNING @ Sun, 21 Jun 2020 17:20:29: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:20:29: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 17:20:29: 19000000 cut: /home/okishinya/chipatlas/results/dm6/SRX030960/SRX030960.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX030960/SRX030960.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX030960/SRX030960.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX030960/SRX030960.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:20:34: 26000000 INFO @ Sun, 21 Jun 2020 17:20:35: 20000000 INFO @ Sun, 21 Jun 2020 17:20:40: 27000000 INFO @ Sun, 21 Jun 2020 17:20:40: 21000000 INFO @ Sun, 21 Jun 2020 17:20:45: 28000000 INFO @ Sun, 21 Jun 2020 17:20:45: 22000000 INFO @ Sun, 21 Jun 2020 17:20:50: 29000000 INFO @ Sun, 21 Jun 2020 17:20:51: 23000000 INFO @ Sun, 21 Jun 2020 17:20:56: 30000000 INFO @ Sun, 21 Jun 2020 17:20:56: 24000000 INFO @ Sun, 21 Jun 2020 17:21:01: 31000000 INFO @ Sun, 21 Jun 2020 17:21:01: 25000000 INFO @ Sun, 21 Jun 2020 17:21:06: 32000000 INFO @ Sun, 21 Jun 2020 17:21:07: 26000000 INFO @ Sun, 21 Jun 2020 17:21:12: 33000000 INFO @ Sun, 21 Jun 2020 17:21:12: 27000000 INFO @ Sun, 21 Jun 2020 17:21:14: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 17:21:14: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 17:21:14: #1 total tags in treatment: 33239656 INFO @ Sun, 21 Jun 2020 17:21:14: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:21:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:21:15: #1 tags after filtering in treatment: 33239656 INFO @ Sun, 21 Jun 2020 17:21:15: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:21:15: #1 finished! INFO @ Sun, 21 Jun 2020 17:21:15: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:21:15: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:21:17: #2 number of paired peaks: 69 WARNING @ Sun, 21 Jun 2020 17:21:17: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:21:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX030960/SRX030960.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX030960/SRX030960.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX030960/SRX030960.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX030960/SRX030960.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:21:17: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:21:22: 29000000 INFO @ Sun, 21 Jun 2020 17:21:27: 30000000 INFO @ Sun, 21 Jun 2020 17:21:32: 31000000 INFO @ Sun, 21 Jun 2020 17:21:37: 32000000 INFO @ Sun, 21 Jun 2020 17:21:42: 33000000 INFO @ Sun, 21 Jun 2020 17:21:44: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 17:21:44: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 17:21:44: #1 total tags in treatment: 33239656 INFO @ Sun, 21 Jun 2020 17:21:44: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:21:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:21:45: #1 tags after filtering in treatment: 33239656 INFO @ Sun, 21 Jun 2020 17:21:45: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:21:45: #1 finished! INFO @ Sun, 21 Jun 2020 17:21:45: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:21:45: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:21:47: #2 number of paired peaks: 69 WARNING @ Sun, 21 Jun 2020 17:21:47: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:21:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX030960/SRX030960.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX030960/SRX030960.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX030960/SRX030960.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX030960/SRX030960.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。