Job ID = 6452661 SRX = SRX025469 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:01:53 prefetch.2.10.7: 1) Downloading 'SRR063877'... 2020-06-21T08:01:53 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:04:09 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:04:10 prefetch.2.10.7: 'SRR063877' is valid 2020-06-21T08:04:10 prefetch.2.10.7: 1) 'SRR063877' was downloaded successfully Read 21352887 spots for SRR063877/SRR063877.sra Written 21352887 spots for SRR063877/SRR063877.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:39 21352887 reads; of these: 21352887 (100.00%) were unpaired; of these: 3488074 (16.34%) aligned 0 times 14876140 (69.67%) aligned exactly 1 time 2988673 (14.00%) aligned >1 times 83.66% overall alignment rate Time searching: 00:03:40 Overall time: 00:03:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3218656 / 17864813 = 0.1802 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:12:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX025469/SRX025469.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX025469/SRX025469.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX025469/SRX025469.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX025469/SRX025469.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:12:21: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:12:21: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:12:26: 1000000 INFO @ Sun, 21 Jun 2020 17:12:32: 2000000 INFO @ Sun, 21 Jun 2020 17:12:37: 3000000 INFO @ Sun, 21 Jun 2020 17:12:42: 4000000 INFO @ Sun, 21 Jun 2020 17:12:47: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:12:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX025469/SRX025469.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX025469/SRX025469.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX025469/SRX025469.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX025469/SRX025469.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:12:51: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:12:51: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:12:53: 6000000 INFO @ Sun, 21 Jun 2020 17:12:57: 1000000 INFO @ Sun, 21 Jun 2020 17:12:59: 7000000 INFO @ Sun, 21 Jun 2020 17:13:03: 2000000 INFO @ Sun, 21 Jun 2020 17:13:05: 8000000 INFO @ Sun, 21 Jun 2020 17:13:09: 3000000 INFO @ Sun, 21 Jun 2020 17:13:11: 9000000 INFO @ Sun, 21 Jun 2020 17:13:15: 4000000 INFO @ Sun, 21 Jun 2020 17:13:17: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:13:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX025469/SRX025469.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX025469/SRX025469.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX025469/SRX025469.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX025469/SRX025469.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:13:21: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:13:21: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:13:21: 5000000 INFO @ Sun, 21 Jun 2020 17:13:23: 11000000 INFO @ Sun, 21 Jun 2020 17:13:27: 1000000 INFO @ Sun, 21 Jun 2020 17:13:27: 6000000 INFO @ Sun, 21 Jun 2020 17:13:29: 12000000 INFO @ Sun, 21 Jun 2020 17:13:33: 7000000 INFO @ Sun, 21 Jun 2020 17:13:33: 2000000 INFO @ Sun, 21 Jun 2020 17:13:35: 13000000 INFO @ Sun, 21 Jun 2020 17:13:39: 8000000 INFO @ Sun, 21 Jun 2020 17:13:40: 3000000 INFO @ Sun, 21 Jun 2020 17:13:41: 14000000 INFO @ Sun, 21 Jun 2020 17:13:45: 9000000 INFO @ Sun, 21 Jun 2020 17:13:46: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 17:13:46: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 17:13:46: #1 total tags in treatment: 14646157 INFO @ Sun, 21 Jun 2020 17:13:46: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:13:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:13:46: 4000000 INFO @ Sun, 21 Jun 2020 17:13:46: #1 tags after filtering in treatment: 14646096 INFO @ Sun, 21 Jun 2020 17:13:46: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:13:46: #1 finished! INFO @ Sun, 21 Jun 2020 17:13:46: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:13:46: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:13:47: #2 number of paired peaks: 62 WARNING @ Sun, 21 Jun 2020 17:13:47: Too few paired peaks (62) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:13:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX025469/SRX025469.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX025469/SRX025469.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX025469/SRX025469.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX025469/SRX025469.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:13:51: 10000000 INFO @ Sun, 21 Jun 2020 17:13:52: 5000000 INFO @ Sun, 21 Jun 2020 17:13:57: 11000000 INFO @ Sun, 21 Jun 2020 17:13:58: 6000000 INFO @ Sun, 21 Jun 2020 17:14:04: 7000000 INFO @ Sun, 21 Jun 2020 17:14:04: 12000000 INFO @ Sun, 21 Jun 2020 17:14:10: 8000000 INFO @ Sun, 21 Jun 2020 17:14:10: 13000000 INFO @ Sun, 21 Jun 2020 17:14:16: 9000000 INFO @ Sun, 21 Jun 2020 17:14:16: 14000000 INFO @ Sun, 21 Jun 2020 17:14:20: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 17:14:20: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 17:14:20: #1 total tags in treatment: 14646157 INFO @ Sun, 21 Jun 2020 17:14:20: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:14:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:14:20: #1 tags after filtering in treatment: 14646096 INFO @ Sun, 21 Jun 2020 17:14:20: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:14:20: #1 finished! INFO @ Sun, 21 Jun 2020 17:14:20: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:14:20: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:14:21: #2 number of paired peaks: 62 WARNING @ Sun, 21 Jun 2020 17:14:21: Too few paired peaks (62) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:14:21: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 17:14:21: 10000000 cut: /home/okishinya/chipatlas/results/dm6/SRX025469/SRX025469.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX025469/SRX025469.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX025469/SRX025469.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX025469/SRX025469.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:14:27: 11000000 INFO @ Sun, 21 Jun 2020 17:14:33: 12000000 INFO @ Sun, 21 Jun 2020 17:14:38: 13000000 INFO @ Sun, 21 Jun 2020 17:14:43: 14000000 INFO @ Sun, 21 Jun 2020 17:14:47: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 17:14:47: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 17:14:47: #1 total tags in treatment: 14646157 INFO @ Sun, 21 Jun 2020 17:14:47: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:14:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:14:47: #1 tags after filtering in treatment: 14646096 INFO @ Sun, 21 Jun 2020 17:14:47: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:14:47: #1 finished! INFO @ Sun, 21 Jun 2020 17:14:47: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:14:47: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:14:48: #2 number of paired peaks: 62 WARNING @ Sun, 21 Jun 2020 17:14:48: Too few paired peaks (62) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:14:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX025469/SRX025469.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX025469/SRX025469.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX025469/SRX025469.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX025469/SRX025469.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。