Job ID = 6529159 SRX = SRX016173 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:05 15188996 reads; of these: 15188996 (100.00%) were unpaired; of these: 1528475 (10.06%) aligned 0 times 11406875 (75.10%) aligned exactly 1 time 2253646 (14.84%) aligned >1 times 89.94% overall alignment rate Time searching: 00:03:05 Overall time: 00:03:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 1702300 / 13660521 = 0.1246 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:35:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX016173/SRX016173.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX016173/SRX016173.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX016173/SRX016173.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX016173/SRX016173.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:35:57: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:35:57: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:36:03: 1000000 INFO @ Tue, 30 Jun 2020 01:36:09: 2000000 INFO @ Tue, 30 Jun 2020 01:36:15: 3000000 INFO @ Tue, 30 Jun 2020 01:36:22: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:36:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX016173/SRX016173.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX016173/SRX016173.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX016173/SRX016173.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX016173/SRX016173.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:36:27: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:36:27: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:36:28: 5000000 INFO @ Tue, 30 Jun 2020 01:36:33: 1000000 INFO @ Tue, 30 Jun 2020 01:36:34: 6000000 INFO @ Tue, 30 Jun 2020 01:36:39: 2000000 INFO @ Tue, 30 Jun 2020 01:36:41: 7000000 INFO @ Tue, 30 Jun 2020 01:36:45: 3000000 INFO @ Tue, 30 Jun 2020 01:36:47: 8000000 INFO @ Tue, 30 Jun 2020 01:36:51: 4000000 INFO @ Tue, 30 Jun 2020 01:36:53: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:36:57: 5000000 INFO @ Tue, 30 Jun 2020 01:36:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX016173/SRX016173.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX016173/SRX016173.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX016173/SRX016173.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX016173/SRX016173.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:36:57: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:36:57: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:37:00: 10000000 INFO @ Tue, 30 Jun 2020 01:37:03: 6000000 INFO @ Tue, 30 Jun 2020 01:37:04: 1000000 INFO @ Tue, 30 Jun 2020 01:37:06: 11000000 INFO @ Tue, 30 Jun 2020 01:37:09: 7000000 INFO @ Tue, 30 Jun 2020 01:37:10: 2000000 INFO @ Tue, 30 Jun 2020 01:37:12: #1 tag size is determined as 36 bps INFO @ Tue, 30 Jun 2020 01:37:12: #1 tag size = 36 INFO @ Tue, 30 Jun 2020 01:37:12: #1 total tags in treatment: 11958221 INFO @ Tue, 30 Jun 2020 01:37:12: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:37:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:37:13: #1 tags after filtering in treatment: 11958206 INFO @ Tue, 30 Jun 2020 01:37:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:37:13: #1 finished! INFO @ Tue, 30 Jun 2020 01:37:13: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:37:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:37:13: #2 number of paired peaks: 92 WARNING @ Tue, 30 Jun 2020 01:37:13: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:37:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX016173/SRX016173.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016173/SRX016173.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016173/SRX016173.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016173/SRX016173.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:37:14: 8000000 INFO @ Tue, 30 Jun 2020 01:37:17: 3000000 INFO @ Tue, 30 Jun 2020 01:37:20: 9000000 INFO @ Tue, 30 Jun 2020 01:37:24: 4000000 INFO @ Tue, 30 Jun 2020 01:37:26: 10000000 INFO @ Tue, 30 Jun 2020 01:37:30: 5000000 INFO @ Tue, 30 Jun 2020 01:37:32: 11000000 INFO @ Tue, 30 Jun 2020 01:37:36: 6000000 INFO @ Tue, 30 Jun 2020 01:37:38: #1 tag size is determined as 36 bps INFO @ Tue, 30 Jun 2020 01:37:38: #1 tag size = 36 INFO @ Tue, 30 Jun 2020 01:37:38: #1 total tags in treatment: 11958221 INFO @ Tue, 30 Jun 2020 01:37:38: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:37:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:37:38: #1 tags after filtering in treatment: 11958206 INFO @ Tue, 30 Jun 2020 01:37:38: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:37:38: #1 finished! INFO @ Tue, 30 Jun 2020 01:37:38: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:37:38: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:37:39: #2 number of paired peaks: 92 WARNING @ Tue, 30 Jun 2020 01:37:39: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:37:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX016173/SRX016173.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016173/SRX016173.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016173/SRX016173.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016173/SRX016173.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:37:43: 7000000 INFO @ Tue, 30 Jun 2020 01:37:49: 8000000 INFO @ Tue, 30 Jun 2020 01:37:55: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 01:38:01: 10000000 INFO @ Tue, 30 Jun 2020 01:38:07: 11000000 INFO @ Tue, 30 Jun 2020 01:38:13: #1 tag size is determined as 36 bps INFO @ Tue, 30 Jun 2020 01:38:13: #1 tag size = 36 INFO @ Tue, 30 Jun 2020 01:38:13: #1 total tags in treatment: 11958221 INFO @ Tue, 30 Jun 2020 01:38:13: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:38:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:38:13: #1 tags after filtering in treatment: 11958206 INFO @ Tue, 30 Jun 2020 01:38:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:38:13: #1 finished! INFO @ Tue, 30 Jun 2020 01:38:13: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:38:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:38:14: #2 number of paired peaks: 92 WARNING @ Tue, 30 Jun 2020 01:38:14: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:38:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX016173/SRX016173.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016173/SRX016173.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016173/SRX016173.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016173/SRX016173.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。