Job ID = 6452567 SRX = SRX016170 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:00:53 prefetch.2.10.7: 1) Downloading 'SRR034738'... 2020-06-21T08:00:53 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:04:43 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:04:44 prefetch.2.10.7: 'SRR034738' is valid 2020-06-21T08:04:44 prefetch.2.10.7: 1) 'SRR034738' was downloaded successfully Read 15544574 spots for SRR034738/SRR034738.sra Written 15544574 spots for SRR034738/SRR034738.sra 2020-06-21T08:05:34 prefetch.2.10.7: 1) Downloading 'SRR034739'... 2020-06-21T08:05:34 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:07:51 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:07:52 prefetch.2.10.7: 'SRR034739' is valid 2020-06-21T08:07:52 prefetch.2.10.7: 1) 'SRR034739' was downloaded successfully Read 13213445 spots for SRR034739/SRR034739.sra Written 13213445 spots for SRR034739/SRR034739.sra 2020-06-21T08:08:35 prefetch.2.10.7: 1) Downloading 'SRR034740'... 2020-06-21T08:08:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:10:51 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:10:51 prefetch.2.10.7: 'SRR034740' is valid 2020-06-21T08:10:51 prefetch.2.10.7: 1) 'SRR034740' was downloaded successfully Read 14604881 spots for SRR034740/SRR034740.sra Written 14604881 spots for SRR034740/SRR034740.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:53 43362900 reads; of these: 43362900 (100.00%) were unpaired; of these: 3287489 (7.58%) aligned 0 times 33577498 (77.43%) aligned exactly 1 time 6497913 (14.98%) aligned >1 times 92.42% overall alignment rate Time searching: 00:07:53 Overall time: 00:07:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 14791052 / 40075411 = 0.3691 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:28:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX016170/SRX016170.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX016170/SRX016170.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX016170/SRX016170.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX016170/SRX016170.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:28:46: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:28:46: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:28:52: 1000000 INFO @ Sun, 21 Jun 2020 17:28:59: 2000000 INFO @ Sun, 21 Jun 2020 17:29:05: 3000000 INFO @ Sun, 21 Jun 2020 17:29:12: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:29:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX016170/SRX016170.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX016170/SRX016170.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX016170/SRX016170.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX016170/SRX016170.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:29:16: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:29:16: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:29:19: 5000000 INFO @ Sun, 21 Jun 2020 17:29:23: 1000000 INFO @ Sun, 21 Jun 2020 17:29:25: 6000000 INFO @ Sun, 21 Jun 2020 17:29:30: 2000000 INFO @ Sun, 21 Jun 2020 17:29:32: 7000000 INFO @ Sun, 21 Jun 2020 17:29:37: 3000000 INFO @ Sun, 21 Jun 2020 17:29:39: 8000000 INFO @ Sun, 21 Jun 2020 17:29:43: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:29:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX016170/SRX016170.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX016170/SRX016170.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX016170/SRX016170.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX016170/SRX016170.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:29:46: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:29:46: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:29:46: 9000000 INFO @ Sun, 21 Jun 2020 17:29:51: 5000000 INFO @ Sun, 21 Jun 2020 17:29:53: 10000000 INFO @ Sun, 21 Jun 2020 17:29:53: 1000000 INFO @ Sun, 21 Jun 2020 17:29:58: 6000000 INFO @ Sun, 21 Jun 2020 17:30:00: 11000000 INFO @ Sun, 21 Jun 2020 17:30:00: 2000000 INFO @ Sun, 21 Jun 2020 17:30:04: 7000000 INFO @ Sun, 21 Jun 2020 17:30:07: 12000000 INFO @ Sun, 21 Jun 2020 17:30:07: 3000000 INFO @ Sun, 21 Jun 2020 17:30:11: 8000000 INFO @ Sun, 21 Jun 2020 17:30:14: 13000000 INFO @ Sun, 21 Jun 2020 17:30:14: 4000000 INFO @ Sun, 21 Jun 2020 17:30:18: 9000000 INFO @ Sun, 21 Jun 2020 17:30:20: 14000000 INFO @ Sun, 21 Jun 2020 17:30:22: 5000000 INFO @ Sun, 21 Jun 2020 17:30:25: 10000000 INFO @ Sun, 21 Jun 2020 17:30:27: 15000000 INFO @ Sun, 21 Jun 2020 17:30:29: 6000000 INFO @ Sun, 21 Jun 2020 17:30:32: 11000000 INFO @ Sun, 21 Jun 2020 17:30:34: 16000000 INFO @ Sun, 21 Jun 2020 17:30:36: 7000000 INFO @ Sun, 21 Jun 2020 17:30:39: 12000000 INFO @ Sun, 21 Jun 2020 17:30:41: 17000000 INFO @ Sun, 21 Jun 2020 17:30:43: 8000000 INFO @ Sun, 21 Jun 2020 17:30:46: 13000000 INFO @ Sun, 21 Jun 2020 17:30:48: 18000000 INFO @ Sun, 21 Jun 2020 17:30:50: 9000000 INFO @ Sun, 21 Jun 2020 17:30:53: 14000000 INFO @ Sun, 21 Jun 2020 17:30:55: 19000000 INFO @ Sun, 21 Jun 2020 17:30:57: 10000000 INFO @ Sun, 21 Jun 2020 17:31:00: 15000000 INFO @ Sun, 21 Jun 2020 17:31:02: 20000000 INFO @ Sun, 21 Jun 2020 17:31:04: 11000000 INFO @ Sun, 21 Jun 2020 17:31:07: 16000000 INFO @ Sun, 21 Jun 2020 17:31:09: 21000000 INFO @ Sun, 21 Jun 2020 17:31:11: 12000000 INFO @ Sun, 21 Jun 2020 17:31:14: 17000000 INFO @ Sun, 21 Jun 2020 17:31:16: 22000000 INFO @ Sun, 21 Jun 2020 17:31:18: 13000000 INFO @ Sun, 21 Jun 2020 17:31:22: 18000000 INFO @ Sun, 21 Jun 2020 17:31:23: 23000000 INFO @ Sun, 21 Jun 2020 17:31:25: 14000000 INFO @ Sun, 21 Jun 2020 17:31:29: 19000000 INFO @ Sun, 21 Jun 2020 17:31:30: 24000000 INFO @ Sun, 21 Jun 2020 17:31:32: 15000000 INFO @ Sun, 21 Jun 2020 17:31:36: 20000000 INFO @ Sun, 21 Jun 2020 17:31:37: 25000000 INFO @ Sun, 21 Jun 2020 17:31:39: 16000000 INFO @ Sun, 21 Jun 2020 17:31:39: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 17:31:39: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 17:31:39: #1 total tags in treatment: 25284359 INFO @ Sun, 21 Jun 2020 17:31:39: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:31:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:31:39: #1 tags after filtering in treatment: 25284356 INFO @ Sun, 21 Jun 2020 17:31:39: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:31:39: #1 finished! INFO @ Sun, 21 Jun 2020 17:31:39: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:31:39: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:31:41: #2 number of paired peaks: 80 WARNING @ Sun, 21 Jun 2020 17:31:41: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:31:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX016170/SRX016170.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016170/SRX016170.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016170/SRX016170.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016170/SRX016170.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:31:43: 21000000 INFO @ Sun, 21 Jun 2020 17:31:45: 17000000 INFO @ Sun, 21 Jun 2020 17:31:50: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:31:52: 18000000 INFO @ Sun, 21 Jun 2020 17:31:57: 23000000 INFO @ Sun, 21 Jun 2020 17:31:59: 19000000 INFO @ Sun, 21 Jun 2020 17:32:04: 24000000 INFO @ Sun, 21 Jun 2020 17:32:06: 20000000 INFO @ Sun, 21 Jun 2020 17:32:11: 25000000 INFO @ Sun, 21 Jun 2020 17:32:13: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 17:32:13: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 17:32:13: #1 total tags in treatment: 25284359 INFO @ Sun, 21 Jun 2020 17:32:13: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:32:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:32:13: 21000000 INFO @ Sun, 21 Jun 2020 17:32:14: #1 tags after filtering in treatment: 25284356 INFO @ Sun, 21 Jun 2020 17:32:14: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:32:14: #1 finished! INFO @ Sun, 21 Jun 2020 17:32:14: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:32:14: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:32:15: #2 number of paired peaks: 80 WARNING @ Sun, 21 Jun 2020 17:32:15: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:32:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX016170/SRX016170.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016170/SRX016170.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016170/SRX016170.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016170/SRX016170.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:32:20: 22000000 INFO @ Sun, 21 Jun 2020 17:32:26: 23000000 INFO @ Sun, 21 Jun 2020 17:32:32: 24000000 INFO @ Sun, 21 Jun 2020 17:32:39: 25000000 INFO @ Sun, 21 Jun 2020 17:32:41: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 17:32:41: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 17:32:41: #1 total tags in treatment: 25284359 INFO @ Sun, 21 Jun 2020 17:32:41: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:32:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:32:41: #1 tags after filtering in treatment: 25284356 INFO @ Sun, 21 Jun 2020 17:32:41: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:32:41: #1 finished! INFO @ Sun, 21 Jun 2020 17:32:41: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:32:41: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:32:43: #2 number of paired peaks: 80 WARNING @ Sun, 21 Jun 2020 17:32:43: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:32:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX016170/SRX016170.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016170/SRX016170.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016170/SRX016170.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016170/SRX016170.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。