Job ID = 6529157 SRX = SRX016169 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:36 13718156 reads; of these: 13718156 (100.00%) were unpaired; of these: 1424686 (10.39%) aligned 0 times 10219844 (74.50%) aligned exactly 1 time 2073626 (15.12%) aligned >1 times 89.61% overall alignment rate Time searching: 00:02:36 Overall time: 00:02:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 1998769 / 12293470 = 0.1626 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:37:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX016169/SRX016169.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX016169/SRX016169.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX016169/SRX016169.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX016169/SRX016169.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:37:37: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:37:37: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:37:42: 1000000 INFO @ Tue, 30 Jun 2020 01:37:47: 2000000 INFO @ Tue, 30 Jun 2020 01:37:53: 3000000 INFO @ Tue, 30 Jun 2020 01:37:58: 4000000 INFO @ Tue, 30 Jun 2020 01:38:03: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:38:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX016169/SRX016169.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX016169/SRX016169.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX016169/SRX016169.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX016169/SRX016169.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:38:07: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:38:07: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:38:08: 6000000 INFO @ Tue, 30 Jun 2020 01:38:13: 1000000 INFO @ Tue, 30 Jun 2020 01:38:13: 7000000 INFO @ Tue, 30 Jun 2020 01:38:18: 2000000 INFO @ Tue, 30 Jun 2020 01:38:19: 8000000 INFO @ Tue, 30 Jun 2020 01:38:24: 3000000 INFO @ Tue, 30 Jun 2020 01:38:25: 9000000 INFO @ Tue, 30 Jun 2020 01:38:29: 4000000 INFO @ Tue, 30 Jun 2020 01:38:30: 10000000 INFO @ Tue, 30 Jun 2020 01:38:32: #1 tag size is determined as 36 bps INFO @ Tue, 30 Jun 2020 01:38:32: #1 tag size = 36 INFO @ Tue, 30 Jun 2020 01:38:32: #1 total tags in treatment: 10294701 INFO @ Tue, 30 Jun 2020 01:38:32: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:38:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:38:33: #1 tags after filtering in treatment: 10294655 INFO @ Tue, 30 Jun 2020 01:38:33: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:38:33: #1 finished! INFO @ Tue, 30 Jun 2020 01:38:33: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:38:33: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:38:34: #2 number of paired peaks: 91 WARNING @ Tue, 30 Jun 2020 01:38:34: Too few paired peaks (91) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:38:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX016169/SRX016169.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016169/SRX016169.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016169/SRX016169.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016169/SRX016169.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:38:34: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:38:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX016169/SRX016169.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX016169/SRX016169.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX016169/SRX016169.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX016169/SRX016169.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:38:37: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:38:37: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:38:40: 6000000 INFO @ Tue, 30 Jun 2020 01:38:42: 1000000 INFO @ Tue, 30 Jun 2020 01:38:45: 7000000 INFO @ Tue, 30 Jun 2020 01:38:48: 2000000 INFO @ Tue, 30 Jun 2020 01:38:51: 8000000 INFO @ Tue, 30 Jun 2020 01:38:53: 3000000 INFO @ Tue, 30 Jun 2020 01:38:58: 9000000 INFO @ Tue, 30 Jun 2020 01:38:59: 4000000 INFO @ Tue, 30 Jun 2020 01:39:04: 10000000 INFO @ Tue, 30 Jun 2020 01:39:05: 5000000 INFO @ Tue, 30 Jun 2020 01:39:06: #1 tag size is determined as 36 bps INFO @ Tue, 30 Jun 2020 01:39:06: #1 tag size = 36 INFO @ Tue, 30 Jun 2020 01:39:06: #1 total tags in treatment: 10294701 INFO @ Tue, 30 Jun 2020 01:39:06: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:39:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:39:07: #1 tags after filtering in treatment: 10294655 INFO @ Tue, 30 Jun 2020 01:39:07: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:39:07: #1 finished! INFO @ Tue, 30 Jun 2020 01:39:07: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:39:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:39:08: #2 number of paired peaks: 91 WARNING @ Tue, 30 Jun 2020 01:39:08: Too few paired peaks (91) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:39:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX016169/SRX016169.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016169/SRX016169.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016169/SRX016169.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016169/SRX016169.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:39:10: 6000000 INFO @ Tue, 30 Jun 2020 01:39:16: 7000000 INFO @ Tue, 30 Jun 2020 01:39:21: 8000000 INFO @ Tue, 30 Jun 2020 01:39:28: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 01:39:34: 10000000 INFO @ Tue, 30 Jun 2020 01:39:36: #1 tag size is determined as 36 bps INFO @ Tue, 30 Jun 2020 01:39:36: #1 tag size = 36 INFO @ Tue, 30 Jun 2020 01:39:36: #1 total tags in treatment: 10294701 INFO @ Tue, 30 Jun 2020 01:39:36: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:39:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:39:37: #1 tags after filtering in treatment: 10294655 INFO @ Tue, 30 Jun 2020 01:39:37: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:39:37: #1 finished! INFO @ Tue, 30 Jun 2020 01:39:37: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:39:37: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:39:38: #2 number of paired peaks: 91 WARNING @ Tue, 30 Jun 2020 01:39:38: Too few paired peaks (91) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:39:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX016169/SRX016169.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016169/SRX016169.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016169/SRX016169.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016169/SRX016169.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。