Job ID = 6529155 SRX = SRX016163 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:06 15285952 reads; of these: 15285952 (100.00%) were unpaired; of these: 2340224 (15.31%) aligned 0 times 11157989 (73.00%) aligned exactly 1 time 1787739 (11.70%) aligned >1 times 84.69% overall alignment rate Time searching: 00:03:06 Overall time: 00:03:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 941230 / 12945728 = 0.0727 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:35:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX016163/SRX016163.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX016163/SRX016163.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX016163/SRX016163.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX016163/SRX016163.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:35:12: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:35:12: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:35:17: 1000000 INFO @ Tue, 30 Jun 2020 01:35:23: 2000000 INFO @ Tue, 30 Jun 2020 01:35:28: 3000000 INFO @ Tue, 30 Jun 2020 01:35:34: 4000000 INFO @ Tue, 30 Jun 2020 01:35:39: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:35:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX016163/SRX016163.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX016163/SRX016163.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX016163/SRX016163.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX016163/SRX016163.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:35:42: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:35:42: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:35:45: 6000000 INFO @ Tue, 30 Jun 2020 01:35:48: 1000000 INFO @ Tue, 30 Jun 2020 01:35:50: 7000000 INFO @ Tue, 30 Jun 2020 01:35:55: 2000000 INFO @ Tue, 30 Jun 2020 01:35:56: 8000000 INFO @ Tue, 30 Jun 2020 01:36:01: 3000000 INFO @ Tue, 30 Jun 2020 01:36:02: 9000000 INFO @ Tue, 30 Jun 2020 01:36:07: 4000000 INFO @ Tue, 30 Jun 2020 01:36:08: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:36:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX016163/SRX016163.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX016163/SRX016163.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX016163/SRX016163.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX016163/SRX016163.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:36:12: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:36:12: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:36:14: 5000000 INFO @ Tue, 30 Jun 2020 01:36:14: 11000000 INFO @ Tue, 30 Jun 2020 01:36:19: 1000000 INFO @ Tue, 30 Jun 2020 01:36:20: 12000000 INFO @ Tue, 30 Jun 2020 01:36:20: 6000000 INFO @ Tue, 30 Jun 2020 01:36:21: #1 tag size is determined as 36 bps INFO @ Tue, 30 Jun 2020 01:36:21: #1 tag size = 36 INFO @ Tue, 30 Jun 2020 01:36:21: #1 total tags in treatment: 12004498 INFO @ Tue, 30 Jun 2020 01:36:21: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:36:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:36:21: #1 tags after filtering in treatment: 12004455 INFO @ Tue, 30 Jun 2020 01:36:21: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:36:21: #1 finished! INFO @ Tue, 30 Jun 2020 01:36:21: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:36:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:36:22: #2 number of paired peaks: 58 WARNING @ Tue, 30 Jun 2020 01:36:22: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:36:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX016163/SRX016163.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016163/SRX016163.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016163/SRX016163.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016163/SRX016163.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:36:26: 2000000 INFO @ Tue, 30 Jun 2020 01:36:27: 7000000 INFO @ Tue, 30 Jun 2020 01:36:33: 3000000 INFO @ Tue, 30 Jun 2020 01:36:33: 8000000 INFO @ Tue, 30 Jun 2020 01:36:40: 9000000 INFO @ Tue, 30 Jun 2020 01:36:40: 4000000 INFO @ Tue, 30 Jun 2020 01:36:46: 5000000 INFO @ Tue, 30 Jun 2020 01:36:46: 10000000 INFO @ Tue, 30 Jun 2020 01:36:53: 6000000 INFO @ Tue, 30 Jun 2020 01:36:53: 11000000 INFO @ Tue, 30 Jun 2020 01:36:59: 7000000 INFO @ Tue, 30 Jun 2020 01:36:59: 12000000 INFO @ Tue, 30 Jun 2020 01:36:59: #1 tag size is determined as 36 bps INFO @ Tue, 30 Jun 2020 01:36:59: #1 tag size = 36 INFO @ Tue, 30 Jun 2020 01:36:59: #1 total tags in treatment: 12004498 INFO @ Tue, 30 Jun 2020 01:36:59: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:36:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:37:00: #1 tags after filtering in treatment: 12004455 INFO @ Tue, 30 Jun 2020 01:37:00: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:37:00: #1 finished! INFO @ Tue, 30 Jun 2020 01:37:00: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:37:00: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:37:01: #2 number of paired peaks: 58 WARNING @ Tue, 30 Jun 2020 01:37:01: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:37:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX016163/SRX016163.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016163/SRX016163.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016163/SRX016163.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016163/SRX016163.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:37:05: 8000000 INFO @ Tue, 30 Jun 2020 01:37:11: 9000000 INFO @ Tue, 30 Jun 2020 01:37:18: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 01:37:25: 11000000 INFO @ Tue, 30 Jun 2020 01:37:31: 12000000 INFO @ Tue, 30 Jun 2020 01:37:32: #1 tag size is determined as 36 bps INFO @ Tue, 30 Jun 2020 01:37:32: #1 tag size = 36 INFO @ Tue, 30 Jun 2020 01:37:32: #1 total tags in treatment: 12004498 INFO @ Tue, 30 Jun 2020 01:37:32: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:37:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:37:32: #1 tags after filtering in treatment: 12004455 INFO @ Tue, 30 Jun 2020 01:37:32: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:37:32: #1 finished! INFO @ Tue, 30 Jun 2020 01:37:32: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:37:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:37:33: #2 number of paired peaks: 58 WARNING @ Tue, 30 Jun 2020 01:37:33: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:37:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX016163/SRX016163.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016163/SRX016163.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016163/SRX016163.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX016163/SRX016163.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。