Job ID = 6452444 SRX = SRX013064 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T07:47:00 prefetch.2.10.7: 1) Downloading 'SRR030330'... 2020-06-21T07:47:00 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T07:47:46 prefetch.2.10.7: HTTPS download succeed 2020-06-21T07:47:46 prefetch.2.10.7: 'SRR030330' is valid 2020-06-21T07:47:46 prefetch.2.10.7: 1) 'SRR030330' was downloaded successfully Read 3500257 spots for SRR030330/SRR030330.sra Written 3500257 spots for SRR030330/SRR030330.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:41 3500257 reads; of these: 3500257 (100.00%) were unpaired; of these: 496125 (14.17%) aligned 0 times 2667486 (76.21%) aligned exactly 1 time 336646 (9.62%) aligned >1 times 85.83% overall alignment rate Time searching: 00:00:41 Overall time: 00:00:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 94268 / 3004132 = 0.0314 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 16:49:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX013064/SRX013064.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX013064/SRX013064.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX013064/SRX013064.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX013064/SRX013064.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 16:49:53: #1 read tag files... INFO @ Sun, 21 Jun 2020 16:49:53: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 16:49:57: 1000000 INFO @ Sun, 21 Jun 2020 16:50:02: 2000000 INFO @ Sun, 21 Jun 2020 16:50:07: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 16:50:07: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 16:50:07: #1 total tags in treatment: 2909864 INFO @ Sun, 21 Jun 2020 16:50:07: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 16:50:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 16:50:08: #1 tags after filtering in treatment: 2909545 INFO @ Sun, 21 Jun 2020 16:50:08: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 16:50:08: #1 finished! INFO @ Sun, 21 Jun 2020 16:50:08: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 16:50:08: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 16:50:08: #2 number of paired peaks: 414 WARNING @ Sun, 21 Jun 2020 16:50:08: Fewer paired peaks (414) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 414 pairs to build model! INFO @ Sun, 21 Jun 2020 16:50:08: start model_add_line... INFO @ Sun, 21 Jun 2020 16:50:08: start X-correlation... INFO @ Sun, 21 Jun 2020 16:50:08: end of X-cor INFO @ Sun, 21 Jun 2020 16:50:08: #2 finished! INFO @ Sun, 21 Jun 2020 16:50:08: #2 predicted fragment length is 131 bps INFO @ Sun, 21 Jun 2020 16:50:08: #2 alternative fragment length(s) may be 131 bps INFO @ Sun, 21 Jun 2020 16:50:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX013064/SRX013064.05_model.r INFO @ Sun, 21 Jun 2020 16:50:08: #3 Call peaks... INFO @ Sun, 21 Jun 2020 16:50:08: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 21 Jun 2020 16:50:15: #3 Call peaks for each chromosome... INFO @ Sun, 21 Jun 2020 16:50:19: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX013064/SRX013064.05_peaks.xls INFO @ Sun, 21 Jun 2020 16:50:19: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX013064/SRX013064.05_peaks.narrowPeak INFO @ Sun, 21 Jun 2020 16:50:19: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX013064/SRX013064.05_summits.bed INFO @ Sun, 21 Jun 2020 16:50:19: Done! pass1 - making usageList (62 chroms): 3 millis pass2 - checking and writing primary data (895 records, 4 fields): 6 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 16:50:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX013064/SRX013064.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX013064/SRX013064.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX013064/SRX013064.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX013064/SRX013064.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 16:50:23: #1 read tag files... INFO @ Sun, 21 Jun 2020 16:50:23: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 16:50:27: 1000000 INFO @ Sun, 21 Jun 2020 16:50:32: 2000000 INFO @ Sun, 21 Jun 2020 16:50:37: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 16:50:37: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 16:50:37: #1 total tags in treatment: 2909864 INFO @ Sun, 21 Jun 2020 16:50:37: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 16:50:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 16:50:37: #1 tags after filtering in treatment: 2909545 INFO @ Sun, 21 Jun 2020 16:50:37: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 16:50:37: #1 finished! INFO @ Sun, 21 Jun 2020 16:50:37: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 16:50:37: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 16:50:38: #2 number of paired peaks: 414 WARNING @ Sun, 21 Jun 2020 16:50:38: Fewer paired peaks (414) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 414 pairs to build model! INFO @ Sun, 21 Jun 2020 16:50:38: start model_add_line... INFO @ Sun, 21 Jun 2020 16:50:38: start X-correlation... INFO @ Sun, 21 Jun 2020 16:50:38: end of X-cor INFO @ Sun, 21 Jun 2020 16:50:38: #2 finished! INFO @ Sun, 21 Jun 2020 16:50:38: #2 predicted fragment length is 131 bps INFO @ Sun, 21 Jun 2020 16:50:38: #2 alternative fragment length(s) may be 131 bps INFO @ Sun, 21 Jun 2020 16:50:38: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX013064/SRX013064.10_model.r INFO @ Sun, 21 Jun 2020 16:50:38: #3 Call peaks... INFO @ Sun, 21 Jun 2020 16:50:38: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 21 Jun 2020 16:50:45: #3 Call peaks for each chromosome... INFO @ Sun, 21 Jun 2020 16:50:48: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX013064/SRX013064.10_peaks.xls INFO @ Sun, 21 Jun 2020 16:50:48: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX013064/SRX013064.10_peaks.narrowPeak INFO @ Sun, 21 Jun 2020 16:50:48: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX013064/SRX013064.10_summits.bed INFO @ Sun, 21 Jun 2020 16:50:48: Done! pass1 - making usageList (51 chroms): 1 millis pass2 - checking and writing primary data (182 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 16:50:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX013064/SRX013064.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX013064/SRX013064.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX013064/SRX013064.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX013064/SRX013064.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 16:50:53: #1 read tag files... INFO @ Sun, 21 Jun 2020 16:50:53: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 16:50:57: 1000000 INFO @ Sun, 21 Jun 2020 16:51:02: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 16:51:07: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 16:51:07: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 16:51:07: #1 total tags in treatment: 2909864 INFO @ Sun, 21 Jun 2020 16:51:07: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 16:51:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 16:51:07: #1 tags after filtering in treatment: 2909545 INFO @ Sun, 21 Jun 2020 16:51:07: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 16:51:07: #1 finished! INFO @ Sun, 21 Jun 2020 16:51:07: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 16:51:07: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 16:51:08: #2 number of paired peaks: 414 WARNING @ Sun, 21 Jun 2020 16:51:08: Fewer paired peaks (414) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 414 pairs to build model! INFO @ Sun, 21 Jun 2020 16:51:08: start model_add_line... INFO @ Sun, 21 Jun 2020 16:51:08: start X-correlation... INFO @ Sun, 21 Jun 2020 16:51:08: end of X-cor INFO @ Sun, 21 Jun 2020 16:51:08: #2 finished! INFO @ Sun, 21 Jun 2020 16:51:08: #2 predicted fragment length is 131 bps INFO @ Sun, 21 Jun 2020 16:51:08: #2 alternative fragment length(s) may be 131 bps INFO @ Sun, 21 Jun 2020 16:51:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX013064/SRX013064.20_model.r INFO @ Sun, 21 Jun 2020 16:51:08: #3 Call peaks... INFO @ Sun, 21 Jun 2020 16:51:08: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 16:51:15: #3 Call peaks for each chromosome... INFO @ Sun, 21 Jun 2020 16:51:18: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX013064/SRX013064.20_peaks.xls INFO @ Sun, 21 Jun 2020 16:51:18: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX013064/SRX013064.20_peaks.narrowPeak INFO @ Sun, 21 Jun 2020 16:51:18: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX013064/SRX013064.20_summits.bed INFO @ Sun, 21 Jun 2020 16:51:18: Done! pass1 - making usageList (28 chroms): 1 millis pass2 - checking and writing primary data (42 records, 4 fields): 2 millis CompletedMACS2peakCalling