Job ID = 6451980 SRX = ERX102379 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T07:16:32 prefetch.2.10.7: 1) Downloading 'ERR126221'... 2020-06-21T07:16:32 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T07:27:11 prefetch.2.10.7: HTTPS download succeed 2020-06-21T07:27:11 prefetch.2.10.7: 1) 'ERR126221' was downloaded successfully Read 25881776 spots for ERR126221/ERR126221.sra Written 25881776 spots for ERR126221/ERR126221.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:58 25881776 reads; of these: 25881776 (100.00%) were unpaired; of these: 1771252 (6.84%) aligned 0 times 22305264 (86.18%) aligned exactly 1 time 1805260 (6.98%) aligned >1 times 93.16% overall alignment rate Time searching: 00:07:58 Overall time: 00:07:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3536983 / 24110524 = 0.1467 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 16:43:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX102379/ERX102379.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX102379/ERX102379.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX102379/ERX102379.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX102379/ERX102379.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 16:43:44: #1 read tag files... INFO @ Sun, 21 Jun 2020 16:43:44: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 16:43:50: 1000000 INFO @ Sun, 21 Jun 2020 16:43:56: 2000000 INFO @ Sun, 21 Jun 2020 16:44:01: 3000000 INFO @ Sun, 21 Jun 2020 16:44:07: 4000000 INFO @ Sun, 21 Jun 2020 16:44:13: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 16:44:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX102379/ERX102379.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX102379/ERX102379.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX102379/ERX102379.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX102379/ERX102379.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 16:44:14: #1 read tag files... INFO @ Sun, 21 Jun 2020 16:44:14: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 16:44:19: 6000000 INFO @ Sun, 21 Jun 2020 16:44:22: 1000000 INFO @ Sun, 21 Jun 2020 16:44:25: 7000000 INFO @ Sun, 21 Jun 2020 16:44:29: 2000000 INFO @ Sun, 21 Jun 2020 16:44:32: 8000000 INFO @ Sun, 21 Jun 2020 16:44:37: 3000000 INFO @ Sun, 21 Jun 2020 16:44:38: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 16:44:44: 4000000 INFO @ Sun, 21 Jun 2020 16:44:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX102379/ERX102379.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX102379/ERX102379.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX102379/ERX102379.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX102379/ERX102379.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 16:44:44: #1 read tag files... INFO @ Sun, 21 Jun 2020 16:44:44: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 16:44:45: 10000000 INFO @ Sun, 21 Jun 2020 16:44:51: 11000000 INFO @ Sun, 21 Jun 2020 16:44:51: 5000000 INFO @ Sun, 21 Jun 2020 16:44:52: 1000000 INFO @ Sun, 21 Jun 2020 16:44:58: 12000000 INFO @ Sun, 21 Jun 2020 16:44:59: 6000000 INFO @ Sun, 21 Jun 2020 16:44:59: 2000000 INFO @ Sun, 21 Jun 2020 16:45:05: 13000000 INFO @ Sun, 21 Jun 2020 16:45:06: 7000000 INFO @ Sun, 21 Jun 2020 16:45:06: 3000000 INFO @ Sun, 21 Jun 2020 16:45:11: 14000000 INFO @ Sun, 21 Jun 2020 16:45:13: 8000000 INFO @ Sun, 21 Jun 2020 16:45:14: 4000000 INFO @ Sun, 21 Jun 2020 16:45:18: 15000000 INFO @ Sun, 21 Jun 2020 16:45:20: 9000000 INFO @ Sun, 21 Jun 2020 16:45:21: 5000000 INFO @ Sun, 21 Jun 2020 16:45:24: 16000000 INFO @ Sun, 21 Jun 2020 16:45:28: 10000000 INFO @ Sun, 21 Jun 2020 16:45:29: 6000000 INFO @ Sun, 21 Jun 2020 16:45:31: 17000000 INFO @ Sun, 21 Jun 2020 16:45:35: 11000000 INFO @ Sun, 21 Jun 2020 16:45:36: 7000000 INFO @ Sun, 21 Jun 2020 16:45:38: 18000000 INFO @ Sun, 21 Jun 2020 16:45:43: 12000000 INFO @ Sun, 21 Jun 2020 16:45:43: 8000000 INFO @ Sun, 21 Jun 2020 16:45:44: 19000000 INFO @ Sun, 21 Jun 2020 16:45:50: 13000000 INFO @ Sun, 21 Jun 2020 16:45:51: 9000000 INFO @ Sun, 21 Jun 2020 16:45:51: 20000000 INFO @ Sun, 21 Jun 2020 16:45:55: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 16:45:55: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 16:45:55: #1 total tags in treatment: 20573541 INFO @ Sun, 21 Jun 2020 16:45:55: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 16:45:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 16:45:56: #1 tags after filtering in treatment: 20573293 INFO @ Sun, 21 Jun 2020 16:45:56: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 16:45:56: #1 finished! INFO @ Sun, 21 Jun 2020 16:45:56: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 16:45:56: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 16:45:57: #2 number of paired peaks: 19 WARNING @ Sun, 21 Jun 2020 16:45:57: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 16:45:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX102379/ERX102379.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX102379/ERX102379.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX102379/ERX102379.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX102379/ERX102379.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 16:45:58: 14000000 INFO @ Sun, 21 Jun 2020 16:45:58: 10000000 INFO @ Sun, 21 Jun 2020 16:46:05: 11000000 INFO @ Sun, 21 Jun 2020 16:46:05: 15000000 INFO @ Sun, 21 Jun 2020 16:46:12: 12000000 INFO @ Sun, 21 Jun 2020 16:46:13: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 16:46:18: 13000000 INFO @ Sun, 21 Jun 2020 16:46:20: 17000000 INFO @ Sun, 21 Jun 2020 16:46:25: 14000000 INFO @ Sun, 21 Jun 2020 16:46:28: 18000000 INFO @ Sun, 21 Jun 2020 16:46:31: 15000000 INFO @ Sun, 21 Jun 2020 16:46:35: 19000000 INFO @ Sun, 21 Jun 2020 16:46:38: 16000000 INFO @ Sun, 21 Jun 2020 16:46:43: 20000000 INFO @ Sun, 21 Jun 2020 16:46:45: 17000000 INFO @ Sun, 21 Jun 2020 16:46:47: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 16:46:47: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 16:46:47: #1 total tags in treatment: 20573541 INFO @ Sun, 21 Jun 2020 16:46:47: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 16:46:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 16:46:48: #1 tags after filtering in treatment: 20573293 INFO @ Sun, 21 Jun 2020 16:46:48: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 16:46:48: #1 finished! INFO @ Sun, 21 Jun 2020 16:46:48: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 16:46:48: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 16:46:49: #2 number of paired peaks: 19 WARNING @ Sun, 21 Jun 2020 16:46:49: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 16:46:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX102379/ERX102379.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX102379/ERX102379.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX102379/ERX102379.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX102379/ERX102379.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 16:46:51: 18000000 INFO @ Sun, 21 Jun 2020 16:46:57: 19000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 16:47:03: 20000000 INFO @ Sun, 21 Jun 2020 16:47:08: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 16:47:08: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 16:47:08: #1 total tags in treatment: 20573541 INFO @ Sun, 21 Jun 2020 16:47:08: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 16:47:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 16:47:08: #1 tags after filtering in treatment: 20573293 INFO @ Sun, 21 Jun 2020 16:47:08: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 16:47:08: #1 finished! INFO @ Sun, 21 Jun 2020 16:47:08: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 16:47:08: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 16:47:10: #2 number of paired peaks: 19 WARNING @ Sun, 21 Jun 2020 16:47:10: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 16:47:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX102379/ERX102379.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX102379/ERX102379.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX102379/ERX102379.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX102379/ERX102379.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling