Job ID = 6451958 SRX = ERX101811 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T07:40:51 prefetch.2.10.7: 1) Downloading 'ERR125650'... 2020-06-21T07:40:51 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T07:47:38 prefetch.2.10.7: HTTPS download succeed 2020-06-21T07:47:39 prefetch.2.10.7: 'ERR125650' is valid 2020-06-21T07:47:39 prefetch.2.10.7: 1) 'ERR125650' was downloaded successfully Read 13564190 spots for ERR125650/ERR125650.sra Written 13564190 spots for ERR125650/ERR125650.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:18 13564190 reads; of these: 13564190 (100.00%) were unpaired; of these: 862673 (6.36%) aligned 0 times 11690458 (86.19%) aligned exactly 1 time 1011059 (7.45%) aligned >1 times 93.64% overall alignment rate Time searching: 00:02:19 Overall time: 00:02:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 1399995 / 12701517 = 0.1102 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 16:54:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX101811/ERX101811.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX101811/ERX101811.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX101811/ERX101811.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX101811/ERX101811.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 16:54:10: #1 read tag files... INFO @ Sun, 21 Jun 2020 16:54:10: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 16:54:15: 1000000 INFO @ Sun, 21 Jun 2020 16:54:21: 2000000 INFO @ Sun, 21 Jun 2020 16:54:26: 3000000 INFO @ Sun, 21 Jun 2020 16:54:31: 4000000 INFO @ Sun, 21 Jun 2020 16:54:37: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 16:54:42: 6000000 INFO @ Sun, 21 Jun 2020 16:54:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX101811/ERX101811.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX101811/ERX101811.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX101811/ERX101811.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX101811/ERX101811.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 16:54:43: #1 read tag files... INFO @ Sun, 21 Jun 2020 16:54:43: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 16:54:48: 7000000 INFO @ Sun, 21 Jun 2020 16:54:49: 1000000 INFO @ Sun, 21 Jun 2020 16:54:54: 8000000 INFO @ Sun, 21 Jun 2020 16:54:55: 2000000 INFO @ Sun, 21 Jun 2020 16:54:59: 9000000 INFO @ Sun, 21 Jun 2020 16:55:01: 3000000 INFO @ Sun, 21 Jun 2020 16:55:06: 10000000 INFO @ Sun, 21 Jun 2020 16:55:06: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 16:55:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX101811/ERX101811.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX101811/ERX101811.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX101811/ERX101811.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX101811/ERX101811.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 16:55:10: #1 read tag files... INFO @ Sun, 21 Jun 2020 16:55:10: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 16:55:11: 11000000 INFO @ Sun, 21 Jun 2020 16:55:12: 5000000 INFO @ Sun, 21 Jun 2020 16:55:13: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 16:55:13: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 16:55:13: #1 total tags in treatment: 11301522 INFO @ Sun, 21 Jun 2020 16:55:13: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 16:55:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 16:55:14: #1 tags after filtering in treatment: 11301250 INFO @ Sun, 21 Jun 2020 16:55:14: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 16:55:14: #1 finished! INFO @ Sun, 21 Jun 2020 16:55:14: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 16:55:14: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 16:55:15: #2 number of paired peaks: 23 WARNING @ Sun, 21 Jun 2020 16:55:15: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 16:55:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX101811/ERX101811.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX101811/ERX101811.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX101811/ERX101811.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX101811/ERX101811.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 16:55:16: 1000000 INFO @ Sun, 21 Jun 2020 16:55:18: 6000000 INFO @ Sun, 21 Jun 2020 16:55:22: 2000000 INFO @ Sun, 21 Jun 2020 16:55:24: 7000000 INFO @ Sun, 21 Jun 2020 16:55:27: 3000000 INFO @ Sun, 21 Jun 2020 16:55:30: 8000000 INFO @ Sun, 21 Jun 2020 16:55:33: 4000000 INFO @ Sun, 21 Jun 2020 16:55:36: 9000000 INFO @ Sun, 21 Jun 2020 16:55:39: 5000000 INFO @ Sun, 21 Jun 2020 16:55:42: 10000000 INFO @ Sun, 21 Jun 2020 16:55:45: 6000000 INFO @ Sun, 21 Jun 2020 16:55:48: 11000000 INFO @ Sun, 21 Jun 2020 16:55:50: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 16:55:50: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 16:55:50: #1 total tags in treatment: 11301522 INFO @ Sun, 21 Jun 2020 16:55:50: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 16:55:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 16:55:50: #1 tags after filtering in treatment: 11301250 INFO @ Sun, 21 Jun 2020 16:55:50: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 16:55:50: #1 finished! INFO @ Sun, 21 Jun 2020 16:55:50: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 16:55:50: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 16:55:50: 7000000 INFO @ Sun, 21 Jun 2020 16:55:51: #2 number of paired peaks: 23 WARNING @ Sun, 21 Jun 2020 16:55:51: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 16:55:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX101811/ERX101811.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX101811/ERX101811.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX101811/ERX101811.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX101811/ERX101811.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 16:55:56: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 16:56:02: 9000000 INFO @ Sun, 21 Jun 2020 16:56:07: 10000000 INFO @ Sun, 21 Jun 2020 16:56:13: 11000000 INFO @ Sun, 21 Jun 2020 16:56:15: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 16:56:15: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 16:56:15: #1 total tags in treatment: 11301522 INFO @ Sun, 21 Jun 2020 16:56:15: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 16:56:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 16:56:15: #1 tags after filtering in treatment: 11301250 INFO @ Sun, 21 Jun 2020 16:56:15: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 16:56:15: #1 finished! INFO @ Sun, 21 Jun 2020 16:56:15: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 16:56:15: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 16:56:16: #2 number of paired peaks: 23 WARNING @ Sun, 21 Jun 2020 16:56:16: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 16:56:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX101811/ERX101811.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX101811/ERX101811.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX101811/ERX101811.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX101811/ERX101811.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。