Job ID = 6626709 SRX = ERX088875 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:22:49 87784639 reads; of these: 87784639 (100.00%) were unpaired; of these: 12570872 (14.32%) aligned 0 times 55189941 (62.87%) aligned exactly 1 time 20023826 (22.81%) aligned >1 times 85.68% overall alignment rate Time searching: 00:22:49 Overall time: 00:22:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 38650162 / 75213767 = 0.5139 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:02:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX088875/ERX088875.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX088875/ERX088875.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX088875/ERX088875.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX088875/ERX088875.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:02:19: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:02:19: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:02:26: 1000000 INFO @ Tue, 14 Jul 2020 09:02:32: 2000000 INFO @ Tue, 14 Jul 2020 09:02:39: 3000000 INFO @ Tue, 14 Jul 2020 09:02:45: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:02:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX088875/ERX088875.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX088875/ERX088875.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX088875/ERX088875.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX088875/ERX088875.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:02:49: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:02:49: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:02:52: 5000000 INFO @ Tue, 14 Jul 2020 09:02:56: 1000000 INFO @ Tue, 14 Jul 2020 09:02:59: 6000000 INFO @ Tue, 14 Jul 2020 09:03:02: 2000000 INFO @ Tue, 14 Jul 2020 09:03:06: 7000000 INFO @ Tue, 14 Jul 2020 09:03:09: 3000000 INFO @ Tue, 14 Jul 2020 09:03:13: 8000000 INFO @ Tue, 14 Jul 2020 09:03:16: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:03:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX088875/ERX088875.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX088875/ERX088875.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX088875/ERX088875.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX088875/ERX088875.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:03:19: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:03:19: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:03:20: 9000000 INFO @ Tue, 14 Jul 2020 09:03:23: 5000000 INFO @ Tue, 14 Jul 2020 09:03:25: 1000000 INFO @ Tue, 14 Jul 2020 09:03:27: 10000000 INFO @ Tue, 14 Jul 2020 09:03:30: 6000000 INFO @ Tue, 14 Jul 2020 09:03:31: 2000000 INFO @ Tue, 14 Jul 2020 09:03:34: 11000000 INFO @ Tue, 14 Jul 2020 09:03:37: 7000000 INFO @ Tue, 14 Jul 2020 09:03:37: 3000000 INFO @ Tue, 14 Jul 2020 09:03:40: 12000000 INFO @ Tue, 14 Jul 2020 09:03:43: 4000000 INFO @ Tue, 14 Jul 2020 09:03:43: 8000000 INFO @ Tue, 14 Jul 2020 09:03:47: 13000000 INFO @ Tue, 14 Jul 2020 09:03:49: 5000000 INFO @ Tue, 14 Jul 2020 09:03:50: 9000000 INFO @ Tue, 14 Jul 2020 09:03:54: 14000000 INFO @ Tue, 14 Jul 2020 09:03:55: 6000000 INFO @ Tue, 14 Jul 2020 09:03:57: 10000000 INFO @ Tue, 14 Jul 2020 09:04:00: 15000000 INFO @ Tue, 14 Jul 2020 09:04:01: 7000000 INFO @ Tue, 14 Jul 2020 09:04:04: 11000000 INFO @ Tue, 14 Jul 2020 09:04:07: 8000000 INFO @ Tue, 14 Jul 2020 09:04:07: 16000000 INFO @ Tue, 14 Jul 2020 09:04:11: 12000000 INFO @ Tue, 14 Jul 2020 09:04:13: 9000000 INFO @ Tue, 14 Jul 2020 09:04:14: 17000000 INFO @ Tue, 14 Jul 2020 09:04:18: 13000000 INFO @ Tue, 14 Jul 2020 09:04:19: 10000000 INFO @ Tue, 14 Jul 2020 09:04:21: 18000000 INFO @ Tue, 14 Jul 2020 09:04:25: 14000000 INFO @ Tue, 14 Jul 2020 09:04:25: 11000000 INFO @ Tue, 14 Jul 2020 09:04:27: 19000000 INFO @ Tue, 14 Jul 2020 09:04:31: 12000000 INFO @ Tue, 14 Jul 2020 09:04:31: 15000000 INFO @ Tue, 14 Jul 2020 09:04:34: 20000000 INFO @ Tue, 14 Jul 2020 09:04:37: 13000000 INFO @ Tue, 14 Jul 2020 09:04:38: 16000000 INFO @ Tue, 14 Jul 2020 09:04:41: 21000000 INFO @ Tue, 14 Jul 2020 09:04:43: 14000000 INFO @ Tue, 14 Jul 2020 09:04:45: 17000000 INFO @ Tue, 14 Jul 2020 09:04:48: 22000000 INFO @ Tue, 14 Jul 2020 09:04:49: 15000000 INFO @ Tue, 14 Jul 2020 09:04:51: 18000000 INFO @ Tue, 14 Jul 2020 09:04:54: 23000000 INFO @ Tue, 14 Jul 2020 09:04:55: 16000000 INFO @ Tue, 14 Jul 2020 09:04:58: 19000000 INFO @ Tue, 14 Jul 2020 09:05:00: 17000000 INFO @ Tue, 14 Jul 2020 09:05:01: 24000000 INFO @ Tue, 14 Jul 2020 09:05:05: 20000000 INFO @ Tue, 14 Jul 2020 09:05:06: 18000000 INFO @ Tue, 14 Jul 2020 09:05:07: 25000000 INFO @ Tue, 14 Jul 2020 09:05:12: 21000000 INFO @ Tue, 14 Jul 2020 09:05:12: 19000000 INFO @ Tue, 14 Jul 2020 09:05:13: 26000000 INFO @ Tue, 14 Jul 2020 09:05:18: 20000000 INFO @ Tue, 14 Jul 2020 09:05:18: 22000000 INFO @ Tue, 14 Jul 2020 09:05:19: 27000000 INFO @ Tue, 14 Jul 2020 09:05:24: 21000000 INFO @ Tue, 14 Jul 2020 09:05:25: 23000000 INFO @ Tue, 14 Jul 2020 09:05:26: 28000000 INFO @ Tue, 14 Jul 2020 09:05:30: 22000000 INFO @ Tue, 14 Jul 2020 09:05:31: 24000000 INFO @ Tue, 14 Jul 2020 09:05:33: 29000000 INFO @ Tue, 14 Jul 2020 09:05:36: 23000000 INFO @ Tue, 14 Jul 2020 09:05:38: 25000000 INFO @ Tue, 14 Jul 2020 09:05:39: 30000000 INFO @ Tue, 14 Jul 2020 09:05:43: 24000000 INFO @ Tue, 14 Jul 2020 09:05:44: 26000000 INFO @ Tue, 14 Jul 2020 09:05:46: 31000000 INFO @ Tue, 14 Jul 2020 09:05:49: 25000000 INFO @ Tue, 14 Jul 2020 09:05:50: 27000000 INFO @ Tue, 14 Jul 2020 09:05:53: 32000000 INFO @ Tue, 14 Jul 2020 09:05:56: 26000000 INFO @ Tue, 14 Jul 2020 09:05:57: 28000000 INFO @ Tue, 14 Jul 2020 09:06:00: 33000000 INFO @ Tue, 14 Jul 2020 09:06:03: 27000000 INFO @ Tue, 14 Jul 2020 09:06:03: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:06:06: 34000000 INFO @ Tue, 14 Jul 2020 09:06:09: 28000000 INFO @ Tue, 14 Jul 2020 09:06:10: 30000000 INFO @ Tue, 14 Jul 2020 09:06:13: 35000000 INFO @ Tue, 14 Jul 2020 09:06:15: 29000000 INFO @ Tue, 14 Jul 2020 09:06:17: 31000000 INFO @ Tue, 14 Jul 2020 09:06:20: 36000000 INFO @ Tue, 14 Jul 2020 09:06:21: 30000000 INFO @ Tue, 14 Jul 2020 09:06:24: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 09:06:24: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 09:06:24: #1 total tags in treatment: 36563605 INFO @ Tue, 14 Jul 2020 09:06:24: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:06:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:06:24: 32000000 INFO @ Tue, 14 Jul 2020 09:06:25: #1 tags after filtering in treatment: 36563551 INFO @ Tue, 14 Jul 2020 09:06:25: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:06:25: #1 finished! INFO @ Tue, 14 Jul 2020 09:06:25: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:06:25: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:06:27: #2 number of paired peaks: 10 WARNING @ Tue, 14 Jul 2020 09:06:27: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:06:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX088875/ERX088875.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088875/ERX088875.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088875/ERX088875.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088875/ERX088875.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:06:27: 31000000 INFO @ Tue, 14 Jul 2020 09:06:31: 33000000 INFO @ Tue, 14 Jul 2020 09:06:33: 32000000 INFO @ Tue, 14 Jul 2020 09:06:37: 34000000 INFO @ Tue, 14 Jul 2020 09:06:39: 33000000 INFO @ Tue, 14 Jul 2020 09:06:44: 35000000 INFO @ Tue, 14 Jul 2020 09:06:45: 34000000 INFO @ Tue, 14 Jul 2020 09:06:51: 35000000 INFO @ Tue, 14 Jul 2020 09:06:51: 36000000 INFO @ Tue, 14 Jul 2020 09:06:55: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 09:06:55: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 09:06:55: #1 total tags in treatment: 36563605 INFO @ Tue, 14 Jul 2020 09:06:55: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:06:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:06:56: #1 tags after filtering in treatment: 36563551 INFO @ Tue, 14 Jul 2020 09:06:56: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:06:56: #1 finished! INFO @ Tue, 14 Jul 2020 09:06:56: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:06:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:06:57: 36000000 INFO @ Tue, 14 Jul 2020 09:06:58: #2 number of paired peaks: 10 WARNING @ Tue, 14 Jul 2020 09:06:58: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:06:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX088875/ERX088875.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088875/ERX088875.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088875/ERX088875.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088875/ERX088875.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:07:00: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 09:07:00: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 09:07:00: #1 total tags in treatment: 36563605 INFO @ Tue, 14 Jul 2020 09:07:00: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:07:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:07:01: #1 tags after filtering in treatment: 36563551 INFO @ Tue, 14 Jul 2020 09:07:01: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:07:01: #1 finished! INFO @ Tue, 14 Jul 2020 09:07:01: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:07:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:07:03: #2 number of paired peaks: 10 WARNING @ Tue, 14 Jul 2020 09:07:03: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:07:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX088875/ERX088875.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088875/ERX088875.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088875/ERX088875.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088875/ERX088875.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。