Job ID = 6626727 SRX = ERX088874 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:34:32 166977586 reads; of these: 166977586 (100.00%) were unpaired; of these: 7457836 (4.47%) aligned 0 times 133896654 (80.19%) aligned exactly 1 time 25623096 (15.35%) aligned >1 times 95.53% overall alignment rate Time searching: 00:34:32 Overall time: 00:34:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 68 files... [bam_rmdupse_core] 109951457 / 159519750 = 0.6893 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:30:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX088874/ERX088874.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX088874/ERX088874.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX088874/ERX088874.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX088874/ERX088874.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:30:18: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:30:18: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:30:24: 1000000 INFO @ Tue, 14 Jul 2020 09:30:29: 2000000 INFO @ Tue, 14 Jul 2020 09:30:35: 3000000 INFO @ Tue, 14 Jul 2020 09:30:40: 4000000 INFO @ Tue, 14 Jul 2020 09:30:46: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:30:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX088874/ERX088874.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX088874/ERX088874.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX088874/ERX088874.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX088874/ERX088874.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:30:48: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:30:48: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:30:51: 6000000 INFO @ Tue, 14 Jul 2020 09:30:54: 1000000 INFO @ Tue, 14 Jul 2020 09:30:57: 7000000 INFO @ Tue, 14 Jul 2020 09:31:00: 2000000 INFO @ Tue, 14 Jul 2020 09:31:03: 8000000 INFO @ Tue, 14 Jul 2020 09:31:06: 3000000 INFO @ Tue, 14 Jul 2020 09:31:09: 9000000 INFO @ Tue, 14 Jul 2020 09:31:12: 4000000 INFO @ Tue, 14 Jul 2020 09:31:15: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:31:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX088874/ERX088874.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX088874/ERX088874.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX088874/ERX088874.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX088874/ERX088874.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:31:18: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:31:18: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:31:18: 5000000 INFO @ Tue, 14 Jul 2020 09:31:21: 11000000 INFO @ Tue, 14 Jul 2020 09:31:24: 1000000 INFO @ Tue, 14 Jul 2020 09:31:25: 6000000 INFO @ Tue, 14 Jul 2020 09:31:27: 12000000 INFO @ Tue, 14 Jul 2020 09:31:30: 2000000 INFO @ Tue, 14 Jul 2020 09:31:31: 7000000 INFO @ Tue, 14 Jul 2020 09:31:33: 13000000 INFO @ Tue, 14 Jul 2020 09:31:36: 3000000 INFO @ Tue, 14 Jul 2020 09:31:37: 8000000 INFO @ Tue, 14 Jul 2020 09:31:39: 14000000 INFO @ Tue, 14 Jul 2020 09:31:42: 4000000 INFO @ Tue, 14 Jul 2020 09:31:43: 9000000 INFO @ Tue, 14 Jul 2020 09:31:45: 15000000 INFO @ Tue, 14 Jul 2020 09:31:48: 5000000 INFO @ Tue, 14 Jul 2020 09:31:49: 10000000 INFO @ Tue, 14 Jul 2020 09:31:51: 16000000 INFO @ Tue, 14 Jul 2020 09:31:54: 6000000 INFO @ Tue, 14 Jul 2020 09:31:55: 11000000 INFO @ Tue, 14 Jul 2020 09:31:57: 17000000 INFO @ Tue, 14 Jul 2020 09:32:00: 7000000 INFO @ Tue, 14 Jul 2020 09:32:01: 12000000 INFO @ Tue, 14 Jul 2020 09:32:02: 18000000 INFO @ Tue, 14 Jul 2020 09:32:06: 8000000 INFO @ Tue, 14 Jul 2020 09:32:07: 13000000 INFO @ Tue, 14 Jul 2020 09:32:08: 19000000 INFO @ Tue, 14 Jul 2020 09:32:12: 9000000 INFO @ Tue, 14 Jul 2020 09:32:13: 14000000 INFO @ Tue, 14 Jul 2020 09:32:14: 20000000 INFO @ Tue, 14 Jul 2020 09:32:18: 10000000 INFO @ Tue, 14 Jul 2020 09:32:19: 15000000 INFO @ Tue, 14 Jul 2020 09:32:20: 21000000 INFO @ Tue, 14 Jul 2020 09:32:24: 11000000 INFO @ Tue, 14 Jul 2020 09:32:25: 16000000 INFO @ Tue, 14 Jul 2020 09:32:26: 22000000 INFO @ Tue, 14 Jul 2020 09:32:30: 12000000 INFO @ Tue, 14 Jul 2020 09:32:31: 17000000 INFO @ Tue, 14 Jul 2020 09:32:32: 23000000 INFO @ Tue, 14 Jul 2020 09:32:36: 13000000 INFO @ Tue, 14 Jul 2020 09:32:37: 18000000 INFO @ Tue, 14 Jul 2020 09:32:38: 24000000 INFO @ Tue, 14 Jul 2020 09:32:42: 14000000 INFO @ Tue, 14 Jul 2020 09:32:43: 19000000 INFO @ Tue, 14 Jul 2020 09:32:44: 25000000 INFO @ Tue, 14 Jul 2020 09:32:48: 15000000 INFO @ Tue, 14 Jul 2020 09:32:49: 20000000 INFO @ Tue, 14 Jul 2020 09:32:50: 26000000 INFO @ Tue, 14 Jul 2020 09:32:54: 16000000 INFO @ Tue, 14 Jul 2020 09:32:55: 21000000 INFO @ Tue, 14 Jul 2020 09:32:56: 27000000 INFO @ Tue, 14 Jul 2020 09:33:00: 17000000 INFO @ Tue, 14 Jul 2020 09:33:01: 22000000 INFO @ Tue, 14 Jul 2020 09:33:03: 28000000 INFO @ Tue, 14 Jul 2020 09:33:06: 18000000 INFO @ Tue, 14 Jul 2020 09:33:07: 23000000 INFO @ Tue, 14 Jul 2020 09:33:09: 29000000 INFO @ Tue, 14 Jul 2020 09:33:12: 19000000 INFO @ Tue, 14 Jul 2020 09:33:13: 24000000 INFO @ Tue, 14 Jul 2020 09:33:15: 30000000 INFO @ Tue, 14 Jul 2020 09:33:18: 20000000 INFO @ Tue, 14 Jul 2020 09:33:19: 25000000 INFO @ Tue, 14 Jul 2020 09:33:21: 31000000 INFO @ Tue, 14 Jul 2020 09:33:24: 21000000 INFO @ Tue, 14 Jul 2020 09:33:25: 26000000 INFO @ Tue, 14 Jul 2020 09:33:27: 32000000 INFO @ Tue, 14 Jul 2020 09:33:30: 22000000 INFO @ Tue, 14 Jul 2020 09:33:31: 27000000 INFO @ Tue, 14 Jul 2020 09:33:33: 33000000 INFO @ Tue, 14 Jul 2020 09:33:36: 23000000 INFO @ Tue, 14 Jul 2020 09:33:37: 28000000 INFO @ Tue, 14 Jul 2020 09:33:39: 34000000 INFO @ Tue, 14 Jul 2020 09:33:42: 24000000 INFO @ Tue, 14 Jul 2020 09:33:43: 29000000 INFO @ Tue, 14 Jul 2020 09:33:45: 35000000 INFO @ Tue, 14 Jul 2020 09:33:48: 25000000 INFO @ Tue, 14 Jul 2020 09:33:49: 30000000 INFO @ Tue, 14 Jul 2020 09:33:51: 36000000 INFO @ Tue, 14 Jul 2020 09:33:54: 26000000 INFO @ Tue, 14 Jul 2020 09:33:55: 31000000 INFO @ Tue, 14 Jul 2020 09:33:57: 37000000 INFO @ Tue, 14 Jul 2020 09:34:00: 27000000 INFO @ Tue, 14 Jul 2020 09:34:01: 32000000 INFO @ Tue, 14 Jul 2020 09:34:03: 38000000 INFO @ Tue, 14 Jul 2020 09:34:06: 28000000 INFO @ Tue, 14 Jul 2020 09:34:07: 33000000 INFO @ Tue, 14 Jul 2020 09:34:09: 39000000 INFO @ Tue, 14 Jul 2020 09:34:13: 29000000 INFO @ Tue, 14 Jul 2020 09:34:13: 34000000 INFO @ Tue, 14 Jul 2020 09:34:15: 40000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:34:19: 30000000 INFO @ Tue, 14 Jul 2020 09:34:19: 35000000 INFO @ Tue, 14 Jul 2020 09:34:21: 41000000 INFO @ Tue, 14 Jul 2020 09:34:25: 31000000 INFO @ Tue, 14 Jul 2020 09:34:25: 36000000 INFO @ Tue, 14 Jul 2020 09:34:27: 42000000 INFO @ Tue, 14 Jul 2020 09:34:31: 32000000 INFO @ Tue, 14 Jul 2020 09:34:31: 37000000 INFO @ Tue, 14 Jul 2020 09:34:33: 43000000 INFO @ Tue, 14 Jul 2020 09:34:37: 33000000 INFO @ Tue, 14 Jul 2020 09:34:37: 38000000 INFO @ Tue, 14 Jul 2020 09:34:39: 44000000 INFO @ Tue, 14 Jul 2020 09:34:43: 34000000 INFO @ Tue, 14 Jul 2020 09:34:44: 39000000 INFO @ Tue, 14 Jul 2020 09:34:45: 45000000 INFO @ Tue, 14 Jul 2020 09:34:49: 35000000 INFO @ Tue, 14 Jul 2020 09:34:50: 40000000 INFO @ Tue, 14 Jul 2020 09:34:51: 46000000 INFO @ Tue, 14 Jul 2020 09:34:55: 36000000 INFO @ Tue, 14 Jul 2020 09:34:55: 41000000 INFO @ Tue, 14 Jul 2020 09:34:57: 47000000 INFO @ Tue, 14 Jul 2020 09:35:01: 37000000 INFO @ Tue, 14 Jul 2020 09:35:02: 42000000 INFO @ Tue, 14 Jul 2020 09:35:03: 48000000 INFO @ Tue, 14 Jul 2020 09:35:07: 38000000 INFO @ Tue, 14 Jul 2020 09:35:08: 43000000 INFO @ Tue, 14 Jul 2020 09:35:09: 49000000 INFO @ Tue, 14 Jul 2020 09:35:12: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 09:35:12: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 09:35:12: #1 total tags in treatment: 49568293 INFO @ Tue, 14 Jul 2020 09:35:12: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:35:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:35:13: 39000000 INFO @ Tue, 14 Jul 2020 09:35:13: #1 tags after filtering in treatment: 49568243 INFO @ Tue, 14 Jul 2020 09:35:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:35:13: #1 finished! INFO @ Tue, 14 Jul 2020 09:35:13: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:35:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:35:14: 44000000 INFO @ Tue, 14 Jul 2020 09:35:16: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 09:35:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:35:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX088874/ERX088874.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088874/ERX088874.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088874/ERX088874.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088874/ERX088874.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:35:19: 40000000 INFO @ Tue, 14 Jul 2020 09:35:20: 45000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 09:35:25: 41000000 INFO @ Tue, 14 Jul 2020 09:35:25: 46000000 INFO @ Tue, 14 Jul 2020 09:35:31: 42000000 INFO @ Tue, 14 Jul 2020 09:35:31: 47000000 INFO @ Tue, 14 Jul 2020 09:35:37: 43000000 INFO @ Tue, 14 Jul 2020 09:35:37: 48000000 INFO @ Tue, 14 Jul 2020 09:35:43: 44000000 INFO @ Tue, 14 Jul 2020 09:35:43: 49000000 INFO @ Tue, 14 Jul 2020 09:35:47: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 09:35:47: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 09:35:47: #1 total tags in treatment: 49568293 INFO @ Tue, 14 Jul 2020 09:35:47: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:35:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:35:48: #1 tags after filtering in treatment: 49568243 INFO @ Tue, 14 Jul 2020 09:35:48: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:35:48: #1 finished! INFO @ Tue, 14 Jul 2020 09:35:48: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:35:48: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:35:49: 45000000 INFO @ Tue, 14 Jul 2020 09:35:51: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 09:35:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:35:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX088874/ERX088874.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088874/ERX088874.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088874/ERX088874.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088874/ERX088874.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:35:54: 46000000 INFO @ Tue, 14 Jul 2020 09:36:00: 47000000 INFO @ Tue, 14 Jul 2020 09:36:06: 48000000 INFO @ Tue, 14 Jul 2020 09:36:12: 49000000 INFO @ Tue, 14 Jul 2020 09:36:15: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 09:36:15: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 09:36:15: #1 total tags in treatment: 49568293 INFO @ Tue, 14 Jul 2020 09:36:15: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:36:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:36:16: #1 tags after filtering in treatment: 49568243 INFO @ Tue, 14 Jul 2020 09:36:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:36:16: #1 finished! INFO @ Tue, 14 Jul 2020 09:36:16: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:36:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:36:19: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 09:36:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:36:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX088874/ERX088874.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088874/ERX088874.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088874/ERX088874.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088874/ERX088874.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling