Job ID = 6626736 SRX = ERX088873 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:36:16 169561362 reads; of these: 169561362 (100.00%) were unpaired; of these: 4668283 (2.75%) aligned 0 times 135992644 (80.20%) aligned exactly 1 time 28900435 (17.04%) aligned >1 times 97.25% overall alignment rate Time searching: 00:36:16 Overall time: 00:36:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 68 files... [bam_rmdupse_core] 115272366 / 164893079 = 0.6991 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:35:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX088873/ERX088873.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX088873/ERX088873.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX088873/ERX088873.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX088873/ERX088873.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:35:31: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:35:31: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:35:37: 1000000 INFO @ Tue, 14 Jul 2020 09:35:42: 2000000 INFO @ Tue, 14 Jul 2020 09:35:48: 3000000 INFO @ Tue, 14 Jul 2020 09:35:54: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:36:00: 5000000 INFO @ Tue, 14 Jul 2020 09:36:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX088873/ERX088873.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX088873/ERX088873.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX088873/ERX088873.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX088873/ERX088873.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:36:01: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:36:01: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:36:05: 6000000 INFO @ Tue, 14 Jul 2020 09:36:07: 1000000 INFO @ Tue, 14 Jul 2020 09:36:11: 7000000 INFO @ Tue, 14 Jul 2020 09:36:13: 2000000 INFO @ Tue, 14 Jul 2020 09:36:17: 8000000 INFO @ Tue, 14 Jul 2020 09:36:19: 3000000 INFO @ Tue, 14 Jul 2020 09:36:23: 9000000 INFO @ Tue, 14 Jul 2020 09:36:25: 4000000 INFO @ Tue, 14 Jul 2020 09:36:29: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:36:30: 5000000 INFO @ Tue, 14 Jul 2020 09:36:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX088873/ERX088873.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX088873/ERX088873.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX088873/ERX088873.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX088873/ERX088873.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:36:31: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:36:31: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:36:35: 11000000 INFO @ Tue, 14 Jul 2020 09:36:36: 6000000 INFO @ Tue, 14 Jul 2020 09:36:37: 1000000 INFO @ Tue, 14 Jul 2020 09:36:40: 12000000 INFO @ Tue, 14 Jul 2020 09:36:42: 7000000 INFO @ Tue, 14 Jul 2020 09:36:43: 2000000 INFO @ Tue, 14 Jul 2020 09:36:46: 13000000 INFO @ Tue, 14 Jul 2020 09:36:48: 8000000 INFO @ Tue, 14 Jul 2020 09:36:50: 3000000 INFO @ Tue, 14 Jul 2020 09:36:52: 14000000 INFO @ Tue, 14 Jul 2020 09:36:54: 9000000 INFO @ Tue, 14 Jul 2020 09:36:56: 4000000 INFO @ Tue, 14 Jul 2020 09:36:58: 15000000 INFO @ Tue, 14 Jul 2020 09:37:00: 10000000 INFO @ Tue, 14 Jul 2020 09:37:02: 5000000 INFO @ Tue, 14 Jul 2020 09:37:03: 16000000 INFO @ Tue, 14 Jul 2020 09:37:06: 11000000 INFO @ Tue, 14 Jul 2020 09:37:08: 6000000 INFO @ Tue, 14 Jul 2020 09:37:09: 17000000 INFO @ Tue, 14 Jul 2020 09:37:12: 12000000 INFO @ Tue, 14 Jul 2020 09:37:14: 7000000 INFO @ Tue, 14 Jul 2020 09:37:15: 18000000 INFO @ Tue, 14 Jul 2020 09:37:18: 13000000 INFO @ Tue, 14 Jul 2020 09:37:20: 8000000 INFO @ Tue, 14 Jul 2020 09:37:21: 19000000 INFO @ Tue, 14 Jul 2020 09:37:24: 14000000 INFO @ Tue, 14 Jul 2020 09:37:26: 9000000 INFO @ Tue, 14 Jul 2020 09:37:27: 20000000 INFO @ Tue, 14 Jul 2020 09:37:30: 15000000 INFO @ Tue, 14 Jul 2020 09:37:32: 10000000 INFO @ Tue, 14 Jul 2020 09:37:32: 21000000 INFO @ Tue, 14 Jul 2020 09:37:36: 16000000 INFO @ Tue, 14 Jul 2020 09:37:38: 11000000 INFO @ Tue, 14 Jul 2020 09:37:38: 22000000 INFO @ Tue, 14 Jul 2020 09:37:42: 17000000 INFO @ Tue, 14 Jul 2020 09:37:43: 12000000 INFO @ Tue, 14 Jul 2020 09:37:44: 23000000 INFO @ Tue, 14 Jul 2020 09:37:48: 18000000 INFO @ Tue, 14 Jul 2020 09:37:49: 13000000 INFO @ Tue, 14 Jul 2020 09:37:50: 24000000 INFO @ Tue, 14 Jul 2020 09:37:54: 19000000 INFO @ Tue, 14 Jul 2020 09:37:55: 14000000 INFO @ Tue, 14 Jul 2020 09:37:56: 25000000 INFO @ Tue, 14 Jul 2020 09:38:00: 20000000 INFO @ Tue, 14 Jul 2020 09:38:01: 15000000 INFO @ Tue, 14 Jul 2020 09:38:01: 26000000 INFO @ Tue, 14 Jul 2020 09:38:06: 21000000 INFO @ Tue, 14 Jul 2020 09:38:07: 16000000 INFO @ Tue, 14 Jul 2020 09:38:07: 27000000 INFO @ Tue, 14 Jul 2020 09:38:12: 22000000 INFO @ Tue, 14 Jul 2020 09:38:13: 28000000 INFO @ Tue, 14 Jul 2020 09:38:13: 17000000 INFO @ Tue, 14 Jul 2020 09:38:18: 23000000 INFO @ Tue, 14 Jul 2020 09:38:19: 29000000 INFO @ Tue, 14 Jul 2020 09:38:19: 18000000 INFO @ Tue, 14 Jul 2020 09:38:24: 24000000 INFO @ Tue, 14 Jul 2020 09:38:25: 30000000 INFO @ Tue, 14 Jul 2020 09:38:25: 19000000 INFO @ Tue, 14 Jul 2020 09:38:30: 25000000 INFO @ Tue, 14 Jul 2020 09:38:31: 31000000 INFO @ Tue, 14 Jul 2020 09:38:31: 20000000 INFO @ Tue, 14 Jul 2020 09:38:36: 26000000 INFO @ Tue, 14 Jul 2020 09:38:36: 32000000 INFO @ Tue, 14 Jul 2020 09:38:37: 21000000 INFO @ Tue, 14 Jul 2020 09:38:42: 27000000 INFO @ Tue, 14 Jul 2020 09:38:42: 33000000 INFO @ Tue, 14 Jul 2020 09:38:43: 22000000 INFO @ Tue, 14 Jul 2020 09:38:48: 28000000 INFO @ Tue, 14 Jul 2020 09:38:48: 34000000 INFO @ Tue, 14 Jul 2020 09:38:49: 23000000 INFO @ Tue, 14 Jul 2020 09:38:54: 35000000 INFO @ Tue, 14 Jul 2020 09:38:54: 29000000 INFO @ Tue, 14 Jul 2020 09:38:55: 24000000 INFO @ Tue, 14 Jul 2020 09:38:59: 36000000 INFO @ Tue, 14 Jul 2020 09:39:00: 30000000 INFO @ Tue, 14 Jul 2020 09:39:01: 25000000 INFO @ Tue, 14 Jul 2020 09:39:05: 37000000 INFO @ Tue, 14 Jul 2020 09:39:06: 31000000 INFO @ Tue, 14 Jul 2020 09:39:07: 26000000 INFO @ Tue, 14 Jul 2020 09:39:11: 38000000 INFO @ Tue, 14 Jul 2020 09:39:11: 32000000 INFO @ Tue, 14 Jul 2020 09:39:13: 27000000 INFO @ Tue, 14 Jul 2020 09:39:16: 39000000 INFO @ Tue, 14 Jul 2020 09:39:17: 33000000 INFO @ Tue, 14 Jul 2020 09:39:19: 28000000 INFO @ Tue, 14 Jul 2020 09:39:22: 40000000 INFO @ Tue, 14 Jul 2020 09:39:23: 34000000 INFO @ Tue, 14 Jul 2020 09:39:25: 29000000 INFO @ Tue, 14 Jul 2020 09:39:28: 41000000 INFO @ Tue, 14 Jul 2020 09:39:29: 35000000 INFO @ Tue, 14 Jul 2020 09:39:31: 30000000 INFO @ Tue, 14 Jul 2020 09:39:34: 42000000 INFO @ Tue, 14 Jul 2020 09:39:35: 36000000 INFO @ Tue, 14 Jul 2020 09:39:37: 31000000 INFO @ Tue, 14 Jul 2020 09:39:40: 43000000 INFO @ Tue, 14 Jul 2020 09:39:41: 37000000 INFO @ Tue, 14 Jul 2020 09:39:43: 32000000 INFO @ Tue, 14 Jul 2020 09:39:45: 44000000 INFO @ Tue, 14 Jul 2020 09:39:46: 38000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:39:48: 33000000 INFO @ Tue, 14 Jul 2020 09:39:51: 45000000 INFO @ Tue, 14 Jul 2020 09:39:52: 39000000 INFO @ Tue, 14 Jul 2020 09:39:54: 34000000 INFO @ Tue, 14 Jul 2020 09:39:57: 46000000 INFO @ Tue, 14 Jul 2020 09:39:58: 40000000 INFO @ Tue, 14 Jul 2020 09:40:00: 35000000 INFO @ Tue, 14 Jul 2020 09:40:03: 47000000 INFO @ Tue, 14 Jul 2020 09:40:04: 41000000 INFO @ Tue, 14 Jul 2020 09:40:06: 36000000 INFO @ Tue, 14 Jul 2020 09:40:08: 48000000 INFO @ Tue, 14 Jul 2020 09:40:10: 42000000 INFO @ Tue, 14 Jul 2020 09:40:12: 37000000 INFO @ Tue, 14 Jul 2020 09:40:14: 49000000 INFO @ Tue, 14 Jul 2020 09:40:16: 43000000 INFO @ Tue, 14 Jul 2020 09:40:18: 38000000 INFO @ Tue, 14 Jul 2020 09:40:18: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 09:40:18: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 09:40:18: #1 total tags in treatment: 49620713 INFO @ Tue, 14 Jul 2020 09:40:18: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:40:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:40:19: #1 tags after filtering in treatment: 49620646 INFO @ Tue, 14 Jul 2020 09:40:19: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:40:19: #1 finished! INFO @ Tue, 14 Jul 2020 09:40:19: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:40:19: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:40:22: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 09:40:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:40:22: Process for pairing-model is terminated! INFO @ Tue, 14 Jul 2020 09:40:22: 44000000 cut: /home/okishinya/chipatlas/results/dm6/ERX088873/ERX088873.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088873/ERX088873.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088873/ERX088873.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088873/ERX088873.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:40:23: 39000000 INFO @ Tue, 14 Jul 2020 09:40:28: 45000000 INFO @ Tue, 14 Jul 2020 09:40:29: 40000000 INFO @ Tue, 14 Jul 2020 09:40:34: 46000000 INFO @ Tue, 14 Jul 2020 09:40:35: 41000000 INFO @ Tue, 14 Jul 2020 09:40:39: 47000000 INFO @ Tue, 14 Jul 2020 09:40:41: 42000000 INFO @ Tue, 14 Jul 2020 09:40:45: 48000000 INFO @ Tue, 14 Jul 2020 09:40:47: 43000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 09:40:51: 49000000 INFO @ Tue, 14 Jul 2020 09:40:53: 44000000 INFO @ Tue, 14 Jul 2020 09:40:55: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 09:40:55: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 09:40:55: #1 total tags in treatment: 49620713 INFO @ Tue, 14 Jul 2020 09:40:55: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:40:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:40:56: #1 tags after filtering in treatment: 49620646 INFO @ Tue, 14 Jul 2020 09:40:56: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:40:56: #1 finished! INFO @ Tue, 14 Jul 2020 09:40:56: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:40:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:40:58: 45000000 INFO @ Tue, 14 Jul 2020 09:40:59: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 09:40:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:40:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX088873/ERX088873.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088873/ERX088873.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088873/ERX088873.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088873/ERX088873.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:41:04: 46000000 INFO @ Tue, 14 Jul 2020 09:41:09: 47000000 INFO @ Tue, 14 Jul 2020 09:41:15: 48000000 INFO @ Tue, 14 Jul 2020 09:41:21: 49000000 INFO @ Tue, 14 Jul 2020 09:41:25: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 09:41:25: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 09:41:25: #1 total tags in treatment: 49620713 INFO @ Tue, 14 Jul 2020 09:41:25: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:41:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:41:26: #1 tags after filtering in treatment: 49620646 INFO @ Tue, 14 Jul 2020 09:41:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:41:26: #1 finished! INFO @ Tue, 14 Jul 2020 09:41:26: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:41:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:41:29: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 09:41:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:41:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX088873/ERX088873.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088873/ERX088873.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088873/ERX088873.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088873/ERX088873.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling