Job ID = 6626737 SRX = ERX088872 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:35:41 168631220 reads; of these: 168631220 (100.00%) were unpaired; of these: 6855068 (4.07%) aligned 0 times 132952914 (78.84%) aligned exactly 1 time 28823238 (17.09%) aligned >1 times 95.93% overall alignment rate Time searching: 00:35:41 Overall time: 00:35:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 68 files... [bam_rmdupse_core] 108377340 / 161776152 = 0.6699 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:35:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX088872/ERX088872.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX088872/ERX088872.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX088872/ERX088872.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX088872/ERX088872.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:35:38: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:35:38: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:35:44: 1000000 INFO @ Tue, 14 Jul 2020 09:35:49: 2000000 INFO @ Tue, 14 Jul 2020 09:35:55: 3000000 INFO @ Tue, 14 Jul 2020 09:36:00: 4000000 INFO @ Tue, 14 Jul 2020 09:36:05: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:36:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX088872/ERX088872.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX088872/ERX088872.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX088872/ERX088872.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX088872/ERX088872.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:36:08: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:36:08: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:36:11: 6000000 INFO @ Tue, 14 Jul 2020 09:36:15: 1000000 INFO @ Tue, 14 Jul 2020 09:36:17: 7000000 INFO @ Tue, 14 Jul 2020 09:36:22: 2000000 INFO @ Tue, 14 Jul 2020 09:36:23: 8000000 INFO @ Tue, 14 Jul 2020 09:36:29: 9000000 INFO @ Tue, 14 Jul 2020 09:36:29: 3000000 INFO @ Tue, 14 Jul 2020 09:36:35: 10000000 INFO @ Tue, 14 Jul 2020 09:36:36: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:36:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX088872/ERX088872.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX088872/ERX088872.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX088872/ERX088872.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX088872/ERX088872.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:36:38: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:36:38: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:36:41: 11000000 INFO @ Tue, 14 Jul 2020 09:36:43: 5000000 INFO @ Tue, 14 Jul 2020 09:36:45: 1000000 INFO @ Tue, 14 Jul 2020 09:36:47: 12000000 INFO @ Tue, 14 Jul 2020 09:36:50: 6000000 INFO @ Tue, 14 Jul 2020 09:36:52: 2000000 INFO @ Tue, 14 Jul 2020 09:36:53: 13000000 INFO @ Tue, 14 Jul 2020 09:36:57: 7000000 INFO @ Tue, 14 Jul 2020 09:36:59: 14000000 INFO @ Tue, 14 Jul 2020 09:36:59: 3000000 INFO @ Tue, 14 Jul 2020 09:37:03: 8000000 INFO @ Tue, 14 Jul 2020 09:37:05: 15000000 INFO @ Tue, 14 Jul 2020 09:37:07: 4000000 INFO @ Tue, 14 Jul 2020 09:37:10: 9000000 INFO @ Tue, 14 Jul 2020 09:37:11: 16000000 INFO @ Tue, 14 Jul 2020 09:37:13: 5000000 INFO @ Tue, 14 Jul 2020 09:37:17: 10000000 INFO @ Tue, 14 Jul 2020 09:37:17: 17000000 INFO @ Tue, 14 Jul 2020 09:37:20: 6000000 INFO @ Tue, 14 Jul 2020 09:37:23: 18000000 INFO @ Tue, 14 Jul 2020 09:37:24: 11000000 INFO @ Tue, 14 Jul 2020 09:37:27: 7000000 INFO @ Tue, 14 Jul 2020 09:37:29: 19000000 INFO @ Tue, 14 Jul 2020 09:37:30: 12000000 INFO @ Tue, 14 Jul 2020 09:37:33: 8000000 INFO @ Tue, 14 Jul 2020 09:37:35: 20000000 INFO @ Tue, 14 Jul 2020 09:37:37: 13000000 INFO @ Tue, 14 Jul 2020 09:37:40: 9000000 INFO @ Tue, 14 Jul 2020 09:37:41: 21000000 INFO @ Tue, 14 Jul 2020 09:37:44: 14000000 INFO @ Tue, 14 Jul 2020 09:37:47: 10000000 INFO @ Tue, 14 Jul 2020 09:37:47: 22000000 INFO @ Tue, 14 Jul 2020 09:37:50: 15000000 INFO @ Tue, 14 Jul 2020 09:37:53: 23000000 INFO @ Tue, 14 Jul 2020 09:37:54: 11000000 INFO @ Tue, 14 Jul 2020 09:37:57: 16000000 INFO @ Tue, 14 Jul 2020 09:37:59: 24000000 INFO @ Tue, 14 Jul 2020 09:38:00: 12000000 INFO @ Tue, 14 Jul 2020 09:38:04: 17000000 INFO @ Tue, 14 Jul 2020 09:38:05: 25000000 INFO @ Tue, 14 Jul 2020 09:38:07: 13000000 INFO @ Tue, 14 Jul 2020 09:38:10: 18000000 INFO @ Tue, 14 Jul 2020 09:38:11: 26000000 INFO @ Tue, 14 Jul 2020 09:38:14: 14000000 INFO @ Tue, 14 Jul 2020 09:38:17: 19000000 INFO @ Tue, 14 Jul 2020 09:38:17: 27000000 INFO @ Tue, 14 Jul 2020 09:38:20: 15000000 INFO @ Tue, 14 Jul 2020 09:38:23: 28000000 INFO @ Tue, 14 Jul 2020 09:38:24: 20000000 INFO @ Tue, 14 Jul 2020 09:38:27: 16000000 INFO @ Tue, 14 Jul 2020 09:38:29: 29000000 INFO @ Tue, 14 Jul 2020 09:38:30: 21000000 INFO @ Tue, 14 Jul 2020 09:38:34: 17000000 INFO @ Tue, 14 Jul 2020 09:38:35: 30000000 INFO @ Tue, 14 Jul 2020 09:38:37: 22000000 INFO @ Tue, 14 Jul 2020 09:38:40: 18000000 INFO @ Tue, 14 Jul 2020 09:38:41: 31000000 INFO @ Tue, 14 Jul 2020 09:38:44: 23000000 INFO @ Tue, 14 Jul 2020 09:38:47: 19000000 INFO @ Tue, 14 Jul 2020 09:38:47: 32000000 INFO @ Tue, 14 Jul 2020 09:38:51: 24000000 INFO @ Tue, 14 Jul 2020 09:38:53: 33000000 INFO @ Tue, 14 Jul 2020 09:38:53: 20000000 INFO @ Tue, 14 Jul 2020 09:38:57: 25000000 INFO @ Tue, 14 Jul 2020 09:38:59: 34000000 INFO @ Tue, 14 Jul 2020 09:39:00: 21000000 INFO @ Tue, 14 Jul 2020 09:39:04: 26000000 INFO @ Tue, 14 Jul 2020 09:39:05: 35000000 INFO @ Tue, 14 Jul 2020 09:39:07: 22000000 INFO @ Tue, 14 Jul 2020 09:39:10: 27000000 INFO @ Tue, 14 Jul 2020 09:39:11: 36000000 INFO @ Tue, 14 Jul 2020 09:39:13: 23000000 INFO @ Tue, 14 Jul 2020 09:39:17: 28000000 INFO @ Tue, 14 Jul 2020 09:39:17: 37000000 INFO @ Tue, 14 Jul 2020 09:39:20: 24000000 INFO @ Tue, 14 Jul 2020 09:39:23: 38000000 INFO @ Tue, 14 Jul 2020 09:39:24: 29000000 INFO @ Tue, 14 Jul 2020 09:39:27: 25000000 INFO @ Tue, 14 Jul 2020 09:39:29: 39000000 INFO @ Tue, 14 Jul 2020 09:39:30: 30000000 INFO @ Tue, 14 Jul 2020 09:39:33: 26000000 INFO @ Tue, 14 Jul 2020 09:39:35: 40000000 INFO @ Tue, 14 Jul 2020 09:39:37: 31000000 INFO @ Tue, 14 Jul 2020 09:39:40: 27000000 INFO @ Tue, 14 Jul 2020 09:39:41: 41000000 INFO @ Tue, 14 Jul 2020 09:39:44: 32000000 INFO @ Tue, 14 Jul 2020 09:39:47: 28000000 INFO @ Tue, 14 Jul 2020 09:39:47: 42000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:39:50: 33000000 INFO @ Tue, 14 Jul 2020 09:39:53: 29000000 INFO @ Tue, 14 Jul 2020 09:39:53: 43000000 INFO @ Tue, 14 Jul 2020 09:39:57: 34000000 INFO @ Tue, 14 Jul 2020 09:39:59: 44000000 INFO @ Tue, 14 Jul 2020 09:40:00: 30000000 INFO @ Tue, 14 Jul 2020 09:40:04: 35000000 INFO @ Tue, 14 Jul 2020 09:40:06: 45000000 INFO @ Tue, 14 Jul 2020 09:40:07: 31000000 INFO @ Tue, 14 Jul 2020 09:40:11: 36000000 INFO @ Tue, 14 Jul 2020 09:40:12: 46000000 INFO @ Tue, 14 Jul 2020 09:40:13: 32000000 INFO @ Tue, 14 Jul 2020 09:40:18: 37000000 INFO @ Tue, 14 Jul 2020 09:40:18: 47000000 INFO @ Tue, 14 Jul 2020 09:40:20: 33000000 INFO @ Tue, 14 Jul 2020 09:40:24: 38000000 INFO @ Tue, 14 Jul 2020 09:40:24: 48000000 INFO @ Tue, 14 Jul 2020 09:40:27: 34000000 INFO @ Tue, 14 Jul 2020 09:40:31: 49000000 INFO @ Tue, 14 Jul 2020 09:40:31: 39000000 INFO @ Tue, 14 Jul 2020 09:40:34: 35000000 INFO @ Tue, 14 Jul 2020 09:40:37: 50000000 INFO @ Tue, 14 Jul 2020 09:40:38: 40000000 INFO @ Tue, 14 Jul 2020 09:40:40: 36000000 INFO @ Tue, 14 Jul 2020 09:40:43: 51000000 INFO @ Tue, 14 Jul 2020 09:40:44: 41000000 INFO @ Tue, 14 Jul 2020 09:40:47: 37000000 INFO @ Tue, 14 Jul 2020 09:40:49: 52000000 INFO @ Tue, 14 Jul 2020 09:40:51: 42000000 INFO @ Tue, 14 Jul 2020 09:40:54: 38000000 INFO @ Tue, 14 Jul 2020 09:40:55: 53000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 09:40:58: 43000000 INFO @ Tue, 14 Jul 2020 09:40:58: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 09:40:58: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 09:40:58: #1 total tags in treatment: 53398812 INFO @ Tue, 14 Jul 2020 09:40:58: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:40:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:40:59: #1 tags after filtering in treatment: 53398754 INFO @ Tue, 14 Jul 2020 09:40:59: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:40:59: #1 finished! INFO @ Tue, 14 Jul 2020 09:40:59: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:40:59: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:41:00: 39000000 INFO @ Tue, 14 Jul 2020 09:41:02: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 09:41:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:41:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX088872/ERX088872.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088872/ERX088872.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088872/ERX088872.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088872/ERX088872.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:41:04: 44000000 INFO @ Tue, 14 Jul 2020 09:41:07: 40000000 INFO @ Tue, 14 Jul 2020 09:41:11: 45000000 INFO @ Tue, 14 Jul 2020 09:41:14: 41000000 INFO @ Tue, 14 Jul 2020 09:41:18: 46000000 INFO @ Tue, 14 Jul 2020 09:41:20: 42000000 INFO @ Tue, 14 Jul 2020 09:41:25: 47000000 INFO @ Tue, 14 Jul 2020 09:41:27: 43000000 INFO @ Tue, 14 Jul 2020 09:41:32: 48000000 INFO @ Tue, 14 Jul 2020 09:41:33: 44000000 INFO @ Tue, 14 Jul 2020 09:41:39: 49000000 INFO @ Tue, 14 Jul 2020 09:41:40: 45000000 INFO @ Tue, 14 Jul 2020 09:41:45: 50000000 INFO @ Tue, 14 Jul 2020 09:41:47: 46000000 INFO @ Tue, 14 Jul 2020 09:41:52: 51000000 INFO @ Tue, 14 Jul 2020 09:41:54: 47000000 INFO @ Tue, 14 Jul 2020 09:41:59: 52000000 INFO @ Tue, 14 Jul 2020 09:42:01: 48000000 INFO @ Tue, 14 Jul 2020 09:42:05: 53000000 INFO @ Tue, 14 Jul 2020 09:42:08: 49000000 INFO @ Tue, 14 Jul 2020 09:42:09: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 09:42:09: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 09:42:09: #1 total tags in treatment: 53398812 INFO @ Tue, 14 Jul 2020 09:42:09: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:42:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:42:10: #1 tags after filtering in treatment: 53398754 INFO @ Tue, 14 Jul 2020 09:42:10: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:42:10: #1 finished! INFO @ Tue, 14 Jul 2020 09:42:10: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:42:10: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:42:13: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 09:42:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:42:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX088872/ERX088872.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088872/ERX088872.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088872/ERX088872.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088872/ERX088872.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:42:14: 50000000 INFO @ Tue, 14 Jul 2020 09:42:20: 51000000 INFO @ Tue, 14 Jul 2020 09:42:27: 52000000 INFO @ Tue, 14 Jul 2020 09:42:33: 53000000 INFO @ Tue, 14 Jul 2020 09:42:36: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 09:42:36: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 09:42:36: #1 total tags in treatment: 53398812 INFO @ Tue, 14 Jul 2020 09:42:36: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:42:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:42:37: #1 tags after filtering in treatment: 53398754 INFO @ Tue, 14 Jul 2020 09:42:37: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:42:37: #1 finished! INFO @ Tue, 14 Jul 2020 09:42:37: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:42:37: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:42:40: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 09:42:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:42:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX088872/ERX088872.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088872/ERX088872.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088872/ERX088872.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088872/ERX088872.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling