Job ID = 6626701 SRX = ERX088871 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:22:49 88936078 reads; of these: 88936078 (100.00%) were unpaired; of these: 11391432 (12.81%) aligned 0 times 57243757 (64.37%) aligned exactly 1 time 20300889 (22.83%) aligned >1 times 87.19% overall alignment rate Time searching: 00:22:49 Overall time: 00:22:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 33242487 / 77544646 = 0.4287 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:02:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX088871/ERX088871.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX088871/ERX088871.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX088871/ERX088871.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX088871/ERX088871.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:02:08: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:02:08: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:02:13: 1000000 INFO @ Tue, 14 Jul 2020 09:02:19: 2000000 INFO @ Tue, 14 Jul 2020 09:02:24: 3000000 INFO @ Tue, 14 Jul 2020 09:02:29: 4000000 INFO @ Tue, 14 Jul 2020 09:02:35: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:02:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX088871/ERX088871.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX088871/ERX088871.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX088871/ERX088871.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX088871/ERX088871.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:02:38: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:02:38: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:02:40: 6000000 INFO @ Tue, 14 Jul 2020 09:02:44: 1000000 INFO @ Tue, 14 Jul 2020 09:02:46: 7000000 INFO @ Tue, 14 Jul 2020 09:02:50: 2000000 INFO @ Tue, 14 Jul 2020 09:02:52: 8000000 INFO @ Tue, 14 Jul 2020 09:02:56: 3000000 INFO @ Tue, 14 Jul 2020 09:02:58: 9000000 INFO @ Tue, 14 Jul 2020 09:03:01: 4000000 INFO @ Tue, 14 Jul 2020 09:03:04: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:03:07: 5000000 INFO @ Tue, 14 Jul 2020 09:03:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX088871/ERX088871.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX088871/ERX088871.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX088871/ERX088871.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX088871/ERX088871.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:03:08: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:03:08: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:03:10: 11000000 INFO @ Tue, 14 Jul 2020 09:03:14: 6000000 INFO @ Tue, 14 Jul 2020 09:03:15: 1000000 INFO @ Tue, 14 Jul 2020 09:03:16: 12000000 INFO @ Tue, 14 Jul 2020 09:03:20: 7000000 INFO @ Tue, 14 Jul 2020 09:03:22: 13000000 INFO @ Tue, 14 Jul 2020 09:03:22: 2000000 INFO @ Tue, 14 Jul 2020 09:03:26: 8000000 INFO @ Tue, 14 Jul 2020 09:03:28: 14000000 INFO @ Tue, 14 Jul 2020 09:03:30: 3000000 INFO @ Tue, 14 Jul 2020 09:03:33: 9000000 INFO @ Tue, 14 Jul 2020 09:03:35: 15000000 INFO @ Tue, 14 Jul 2020 09:03:37: 4000000 INFO @ Tue, 14 Jul 2020 09:03:39: 10000000 INFO @ Tue, 14 Jul 2020 09:03:41: 16000000 INFO @ Tue, 14 Jul 2020 09:03:44: 5000000 INFO @ Tue, 14 Jul 2020 09:03:45: 11000000 INFO @ Tue, 14 Jul 2020 09:03:47: 17000000 INFO @ Tue, 14 Jul 2020 09:03:51: 6000000 INFO @ Tue, 14 Jul 2020 09:03:51: 12000000 INFO @ Tue, 14 Jul 2020 09:03:54: 18000000 INFO @ Tue, 14 Jul 2020 09:03:58: 13000000 INFO @ Tue, 14 Jul 2020 09:03:58: 7000000 INFO @ Tue, 14 Jul 2020 09:04:00: 19000000 INFO @ Tue, 14 Jul 2020 09:04:04: 14000000 INFO @ Tue, 14 Jul 2020 09:04:05: 8000000 INFO @ Tue, 14 Jul 2020 09:04:06: 20000000 INFO @ Tue, 14 Jul 2020 09:04:10: 15000000 INFO @ Tue, 14 Jul 2020 09:04:12: 21000000 INFO @ Tue, 14 Jul 2020 09:04:12: 9000000 INFO @ Tue, 14 Jul 2020 09:04:17: 16000000 INFO @ Tue, 14 Jul 2020 09:04:19: 22000000 INFO @ Tue, 14 Jul 2020 09:04:19: 10000000 INFO @ Tue, 14 Jul 2020 09:04:23: 17000000 INFO @ Tue, 14 Jul 2020 09:04:25: 23000000 INFO @ Tue, 14 Jul 2020 09:04:26: 11000000 INFO @ Tue, 14 Jul 2020 09:04:29: 18000000 INFO @ Tue, 14 Jul 2020 09:04:31: 24000000 INFO @ Tue, 14 Jul 2020 09:04:33: 12000000 INFO @ Tue, 14 Jul 2020 09:04:36: 19000000 INFO @ Tue, 14 Jul 2020 09:04:38: 25000000 INFO @ Tue, 14 Jul 2020 09:04:40: 13000000 INFO @ Tue, 14 Jul 2020 09:04:42: 20000000 INFO @ Tue, 14 Jul 2020 09:04:44: 26000000 INFO @ Tue, 14 Jul 2020 09:04:47: 14000000 INFO @ Tue, 14 Jul 2020 09:04:48: 21000000 INFO @ Tue, 14 Jul 2020 09:04:50: 27000000 INFO @ Tue, 14 Jul 2020 09:04:54: 15000000 INFO @ Tue, 14 Jul 2020 09:04:54: 22000000 INFO @ Tue, 14 Jul 2020 09:04:56: 28000000 INFO @ Tue, 14 Jul 2020 09:05:01: 23000000 INFO @ Tue, 14 Jul 2020 09:05:01: 16000000 INFO @ Tue, 14 Jul 2020 09:05:03: 29000000 INFO @ Tue, 14 Jul 2020 09:05:07: 24000000 INFO @ Tue, 14 Jul 2020 09:05:08: 17000000 INFO @ Tue, 14 Jul 2020 09:05:09: 30000000 INFO @ Tue, 14 Jul 2020 09:05:13: 25000000 INFO @ Tue, 14 Jul 2020 09:05:15: 18000000 INFO @ Tue, 14 Jul 2020 09:05:15: 31000000 INFO @ Tue, 14 Jul 2020 09:05:20: 26000000 INFO @ Tue, 14 Jul 2020 09:05:21: 32000000 INFO @ Tue, 14 Jul 2020 09:05:22: 19000000 INFO @ Tue, 14 Jul 2020 09:05:26: 27000000 INFO @ Tue, 14 Jul 2020 09:05:28: 33000000 INFO @ Tue, 14 Jul 2020 09:05:29: 20000000 INFO @ Tue, 14 Jul 2020 09:05:32: 28000000 INFO @ Tue, 14 Jul 2020 09:05:34: 34000000 INFO @ Tue, 14 Jul 2020 09:05:36: 21000000 INFO @ Tue, 14 Jul 2020 09:05:38: 29000000 INFO @ Tue, 14 Jul 2020 09:05:40: 35000000 INFO @ Tue, 14 Jul 2020 09:05:43: 22000000 INFO @ Tue, 14 Jul 2020 09:05:45: 30000000 INFO @ Tue, 14 Jul 2020 09:05:47: 36000000 INFO @ Tue, 14 Jul 2020 09:05:50: 23000000 INFO @ Tue, 14 Jul 2020 09:05:51: 31000000 INFO @ Tue, 14 Jul 2020 09:05:53: 37000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:05:57: 32000000 INFO @ Tue, 14 Jul 2020 09:05:57: 24000000 INFO @ Tue, 14 Jul 2020 09:05:59: 38000000 INFO @ Tue, 14 Jul 2020 09:06:03: 33000000 INFO @ Tue, 14 Jul 2020 09:06:04: 25000000 INFO @ Tue, 14 Jul 2020 09:06:06: 39000000 INFO @ Tue, 14 Jul 2020 09:06:09: 34000000 INFO @ Tue, 14 Jul 2020 09:06:11: 26000000 INFO @ Tue, 14 Jul 2020 09:06:12: 40000000 INFO @ Tue, 14 Jul 2020 09:06:15: 35000000 INFO @ Tue, 14 Jul 2020 09:06:18: 27000000 INFO @ Tue, 14 Jul 2020 09:06:18: 41000000 INFO @ Tue, 14 Jul 2020 09:06:22: 36000000 INFO @ Tue, 14 Jul 2020 09:06:24: 42000000 INFO @ Tue, 14 Jul 2020 09:06:25: 28000000 INFO @ Tue, 14 Jul 2020 09:06:28: 37000000 INFO @ Tue, 14 Jul 2020 09:06:30: 43000000 INFO @ Tue, 14 Jul 2020 09:06:32: 29000000 INFO @ Tue, 14 Jul 2020 09:06:34: 38000000 INFO @ Tue, 14 Jul 2020 09:06:37: 44000000 INFO @ Tue, 14 Jul 2020 09:06:38: 30000000 INFO @ Tue, 14 Jul 2020 09:06:39: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 09:06:39: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 09:06:39: #1 total tags in treatment: 44302159 INFO @ Tue, 14 Jul 2020 09:06:39: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:06:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:06:40: #1 tags after filtering in treatment: 44302096 INFO @ Tue, 14 Jul 2020 09:06:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:06:40: #1 finished! INFO @ Tue, 14 Jul 2020 09:06:40: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:06:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:06:41: 39000000 INFO @ Tue, 14 Jul 2020 09:06:43: #2 number of paired peaks: 5 WARNING @ Tue, 14 Jul 2020 09:06:43: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:06:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX088871/ERX088871.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088871/ERX088871.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088871/ERX088871.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088871/ERX088871.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:06:45: 31000000 INFO @ Tue, 14 Jul 2020 09:06:47: 40000000 INFO @ Tue, 14 Jul 2020 09:06:52: 32000000 INFO @ Tue, 14 Jul 2020 09:06:53: 41000000 INFO @ Tue, 14 Jul 2020 09:06:59: 42000000 INFO @ Tue, 14 Jul 2020 09:06:59: 33000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 09:07:05: 43000000 INFO @ Tue, 14 Jul 2020 09:07:06: 34000000 INFO @ Tue, 14 Jul 2020 09:07:11: 44000000 INFO @ Tue, 14 Jul 2020 09:07:13: 35000000 INFO @ Tue, 14 Jul 2020 09:07:13: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 09:07:13: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 09:07:13: #1 total tags in treatment: 44302159 INFO @ Tue, 14 Jul 2020 09:07:13: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:07:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:07:14: #1 tags after filtering in treatment: 44302096 INFO @ Tue, 14 Jul 2020 09:07:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:07:14: #1 finished! INFO @ Tue, 14 Jul 2020 09:07:14: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:07:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:07:17: #2 number of paired peaks: 5 WARNING @ Tue, 14 Jul 2020 09:07:17: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:07:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX088871/ERX088871.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088871/ERX088871.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088871/ERX088871.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088871/ERX088871.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:07:19: 36000000 INFO @ Tue, 14 Jul 2020 09:07:26: 37000000 INFO @ Tue, 14 Jul 2020 09:07:33: 38000000 INFO @ Tue, 14 Jul 2020 09:07:40: 39000000 INFO @ Tue, 14 Jul 2020 09:07:46: 40000000 INFO @ Tue, 14 Jul 2020 09:07:53: 41000000 INFO @ Tue, 14 Jul 2020 09:07:59: 42000000 INFO @ Tue, 14 Jul 2020 09:08:06: 43000000 INFO @ Tue, 14 Jul 2020 09:08:12: 44000000 INFO @ Tue, 14 Jul 2020 09:08:15: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 09:08:15: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 09:08:15: #1 total tags in treatment: 44302159 INFO @ Tue, 14 Jul 2020 09:08:15: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:08:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:08:15: #1 tags after filtering in treatment: 44302096 INFO @ Tue, 14 Jul 2020 09:08:15: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:08:15: #1 finished! INFO @ Tue, 14 Jul 2020 09:08:15: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:08:15: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:08:18: #2 number of paired peaks: 5 WARNING @ Tue, 14 Jul 2020 09:08:18: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:08:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX088871/ERX088871.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088871/ERX088871.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088871/ERX088871.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088871/ERX088871.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling