Job ID = 6626729 SRX = ERX088868 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:40:43 166371392 reads; of these: 166371392 (100.00%) were unpaired; of these: 6433201 (3.87%) aligned 0 times 131892557 (79.28%) aligned exactly 1 time 28045634 (16.86%) aligned >1 times 96.13% overall alignment rate Time searching: 00:40:43 Overall time: 00:40:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 68 files... [bam_rmdupse_core] 103386258 / 159938191 = 0.6464 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:39:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX088868/ERX088868.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX088868/ERX088868.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX088868/ERX088868.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX088868/ERX088868.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:39:10: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:39:10: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:39:17: 1000000 INFO @ Tue, 14 Jul 2020 09:39:25: 2000000 INFO @ Tue, 14 Jul 2020 09:39:33: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:39:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX088868/ERX088868.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX088868/ERX088868.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX088868/ERX088868.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX088868/ERX088868.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:39:39: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:39:39: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:39:41: 4000000 INFO @ Tue, 14 Jul 2020 09:39:45: 1000000 INFO @ Tue, 14 Jul 2020 09:39:49: 5000000 INFO @ Tue, 14 Jul 2020 09:39:52: 2000000 INFO @ Tue, 14 Jul 2020 09:39:57: 6000000 INFO @ Tue, 14 Jul 2020 09:39:58: 3000000 INFO @ Tue, 14 Jul 2020 09:40:04: 4000000 INFO @ Tue, 14 Jul 2020 09:40:05: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:40:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX088868/ERX088868.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX088868/ERX088868.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX088868/ERX088868.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX088868/ERX088868.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:40:09: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:40:09: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:40:10: 5000000 INFO @ Tue, 14 Jul 2020 09:40:13: 8000000 INFO @ Tue, 14 Jul 2020 09:40:16: 1000000 INFO @ Tue, 14 Jul 2020 09:40:16: 6000000 INFO @ Tue, 14 Jul 2020 09:40:21: 9000000 INFO @ Tue, 14 Jul 2020 09:40:22: 7000000 INFO @ Tue, 14 Jul 2020 09:40:22: 2000000 INFO @ Tue, 14 Jul 2020 09:40:28: 8000000 INFO @ Tue, 14 Jul 2020 09:40:28: 3000000 INFO @ Tue, 14 Jul 2020 09:40:30: 10000000 INFO @ Tue, 14 Jul 2020 09:40:34: 9000000 INFO @ Tue, 14 Jul 2020 09:40:35: 4000000 INFO @ Tue, 14 Jul 2020 09:40:39: 11000000 INFO @ Tue, 14 Jul 2020 09:40:40: 10000000 INFO @ Tue, 14 Jul 2020 09:40:41: 5000000 INFO @ Tue, 14 Jul 2020 09:40:46: 11000000 INFO @ Tue, 14 Jul 2020 09:40:47: 12000000 INFO @ Tue, 14 Jul 2020 09:40:47: 6000000 INFO @ Tue, 14 Jul 2020 09:40:52: 12000000 INFO @ Tue, 14 Jul 2020 09:40:53: 7000000 INFO @ Tue, 14 Jul 2020 09:40:55: 13000000 INFO @ Tue, 14 Jul 2020 09:40:58: 13000000 INFO @ Tue, 14 Jul 2020 09:41:00: 8000000 INFO @ Tue, 14 Jul 2020 09:41:02: 14000000 INFO @ Tue, 14 Jul 2020 09:41:04: 14000000 INFO @ Tue, 14 Jul 2020 09:41:06: 9000000 INFO @ Tue, 14 Jul 2020 09:41:10: 15000000 INFO @ Tue, 14 Jul 2020 09:41:10: 15000000 INFO @ Tue, 14 Jul 2020 09:41:12: 10000000 INFO @ Tue, 14 Jul 2020 09:41:17: 16000000 INFO @ Tue, 14 Jul 2020 09:41:18: 16000000 INFO @ Tue, 14 Jul 2020 09:41:19: 11000000 INFO @ Tue, 14 Jul 2020 09:41:23: 17000000 INFO @ Tue, 14 Jul 2020 09:41:25: 12000000 INFO @ Tue, 14 Jul 2020 09:41:26: 17000000 INFO @ Tue, 14 Jul 2020 09:41:29: 18000000 INFO @ Tue, 14 Jul 2020 09:41:31: 13000000 INFO @ Tue, 14 Jul 2020 09:41:34: 18000000 INFO @ Tue, 14 Jul 2020 09:41:35: 19000000 INFO @ Tue, 14 Jul 2020 09:41:37: 14000000 INFO @ Tue, 14 Jul 2020 09:41:41: 20000000 INFO @ Tue, 14 Jul 2020 09:41:42: 19000000 INFO @ Tue, 14 Jul 2020 09:41:43: 15000000 INFO @ Tue, 14 Jul 2020 09:41:47: 21000000 INFO @ Tue, 14 Jul 2020 09:41:48: 20000000 INFO @ Tue, 14 Jul 2020 09:41:49: 16000000 INFO @ Tue, 14 Jul 2020 09:41:54: 22000000 INFO @ Tue, 14 Jul 2020 09:41:54: 21000000 INFO @ Tue, 14 Jul 2020 09:41:56: 17000000 INFO @ Tue, 14 Jul 2020 09:42:00: 23000000 INFO @ Tue, 14 Jul 2020 09:42:00: 22000000 INFO @ Tue, 14 Jul 2020 09:42:02: 18000000 INFO @ Tue, 14 Jul 2020 09:42:06: 24000000 INFO @ Tue, 14 Jul 2020 09:42:06: 23000000 INFO @ Tue, 14 Jul 2020 09:42:08: 19000000 INFO @ Tue, 14 Jul 2020 09:42:12: 25000000 INFO @ Tue, 14 Jul 2020 09:42:12: 24000000 INFO @ Tue, 14 Jul 2020 09:42:14: 20000000 INFO @ Tue, 14 Jul 2020 09:42:18: 26000000 INFO @ Tue, 14 Jul 2020 09:42:19: 25000000 INFO @ Tue, 14 Jul 2020 09:42:20: 21000000 INFO @ Tue, 14 Jul 2020 09:42:24: 27000000 INFO @ Tue, 14 Jul 2020 09:42:25: 26000000 INFO @ Tue, 14 Jul 2020 09:42:26: 22000000 INFO @ Tue, 14 Jul 2020 09:42:31: 28000000 INFO @ Tue, 14 Jul 2020 09:42:31: 27000000 INFO @ Tue, 14 Jul 2020 09:42:32: 23000000 INFO @ Tue, 14 Jul 2020 09:42:37: 29000000 INFO @ Tue, 14 Jul 2020 09:42:37: 28000000 INFO @ Tue, 14 Jul 2020 09:42:39: 24000000 INFO @ Tue, 14 Jul 2020 09:42:43: 30000000 INFO @ Tue, 14 Jul 2020 09:42:43: 29000000 INFO @ Tue, 14 Jul 2020 09:42:45: 25000000 INFO @ Tue, 14 Jul 2020 09:42:49: 31000000 INFO @ Tue, 14 Jul 2020 09:42:49: 30000000 INFO @ Tue, 14 Jul 2020 09:42:51: 26000000 INFO @ Tue, 14 Jul 2020 09:42:55: 32000000 INFO @ Tue, 14 Jul 2020 09:42:55: 31000000 INFO @ Tue, 14 Jul 2020 09:42:57: 27000000 INFO @ Tue, 14 Jul 2020 09:43:01: 33000000 INFO @ Tue, 14 Jul 2020 09:43:01: 32000000 INFO @ Tue, 14 Jul 2020 09:43:04: 28000000 INFO @ Tue, 14 Jul 2020 09:43:07: 33000000 INFO @ Tue, 14 Jul 2020 09:43:07: 34000000 INFO @ Tue, 14 Jul 2020 09:43:10: 29000000 INFO @ Tue, 14 Jul 2020 09:43:13: 34000000 INFO @ Tue, 14 Jul 2020 09:43:13: 35000000 INFO @ Tue, 14 Jul 2020 09:43:16: 30000000 INFO @ Tue, 14 Jul 2020 09:43:19: 35000000 INFO @ Tue, 14 Jul 2020 09:43:19: 36000000 INFO @ Tue, 14 Jul 2020 09:43:22: 31000000 INFO @ Tue, 14 Jul 2020 09:43:25: 36000000 INFO @ Tue, 14 Jul 2020 09:43:25: 37000000 INFO @ Tue, 14 Jul 2020 09:43:28: 32000000 INFO @ Tue, 14 Jul 2020 09:43:31: 37000000 INFO @ Tue, 14 Jul 2020 09:43:31: 38000000 INFO @ Tue, 14 Jul 2020 09:43:35: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:43:37: 39000000 INFO @ Tue, 14 Jul 2020 09:43:37: 38000000 INFO @ Tue, 14 Jul 2020 09:43:41: 34000000 INFO @ Tue, 14 Jul 2020 09:43:43: 40000000 INFO @ Tue, 14 Jul 2020 09:43:44: 39000000 INFO @ Tue, 14 Jul 2020 09:43:46: 35000000 INFO @ Tue, 14 Jul 2020 09:43:49: 41000000 INFO @ Tue, 14 Jul 2020 09:43:50: 40000000 INFO @ Tue, 14 Jul 2020 09:43:52: 36000000 INFO @ Tue, 14 Jul 2020 09:43:55: 42000000 INFO @ Tue, 14 Jul 2020 09:43:56: 41000000 INFO @ Tue, 14 Jul 2020 09:43:59: 37000000 INFO @ Tue, 14 Jul 2020 09:44:01: 43000000 INFO @ Tue, 14 Jul 2020 09:44:03: 42000000 INFO @ Tue, 14 Jul 2020 09:44:05: 38000000 INFO @ Tue, 14 Jul 2020 09:44:07: 44000000 INFO @ Tue, 14 Jul 2020 09:44:09: 43000000 INFO @ Tue, 14 Jul 2020 09:44:11: 39000000 INFO @ Tue, 14 Jul 2020 09:44:13: 45000000 INFO @ Tue, 14 Jul 2020 09:44:15: 44000000 INFO @ Tue, 14 Jul 2020 09:44:17: 40000000 INFO @ Tue, 14 Jul 2020 09:44:19: 46000000 INFO @ Tue, 14 Jul 2020 09:44:22: 45000000 INFO @ Tue, 14 Jul 2020 09:44:24: 41000000 INFO @ Tue, 14 Jul 2020 09:44:25: 47000000 INFO @ Tue, 14 Jul 2020 09:44:28: 46000000 INFO @ Tue, 14 Jul 2020 09:44:30: 42000000 INFO @ Tue, 14 Jul 2020 09:44:31: 48000000 INFO @ Tue, 14 Jul 2020 09:44:34: 47000000 INFO @ Tue, 14 Jul 2020 09:44:36: 43000000 INFO @ Tue, 14 Jul 2020 09:44:37: 49000000 INFO @ Tue, 14 Jul 2020 09:44:40: 48000000 INFO @ Tue, 14 Jul 2020 09:44:43: 44000000 INFO @ Tue, 14 Jul 2020 09:44:43: 50000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 09:44:46: 49000000 INFO @ Tue, 14 Jul 2020 09:44:49: 45000000 INFO @ Tue, 14 Jul 2020 09:44:49: 51000000 INFO @ Tue, 14 Jul 2020 09:44:53: 50000000 INFO @ Tue, 14 Jul 2020 09:44:55: 46000000 INFO @ Tue, 14 Jul 2020 09:44:55: 52000000 INFO @ Tue, 14 Jul 2020 09:44:58: 51000000 INFO @ Tue, 14 Jul 2020 09:45:01: 47000000 INFO @ Tue, 14 Jul 2020 09:45:01: 53000000 INFO @ Tue, 14 Jul 2020 09:45:04: 52000000 INFO @ Tue, 14 Jul 2020 09:45:07: 48000000 INFO @ Tue, 14 Jul 2020 09:45:07: 54000000 INFO @ Tue, 14 Jul 2020 09:45:10: 53000000 INFO @ Tue, 14 Jul 2020 09:45:13: 49000000 INFO @ Tue, 14 Jul 2020 09:45:13: 55000000 INFO @ Tue, 14 Jul 2020 09:45:16: 54000000 INFO @ Tue, 14 Jul 2020 09:45:19: 50000000 INFO @ Tue, 14 Jul 2020 09:45:19: 56000000 INFO @ Tue, 14 Jul 2020 09:45:23: 55000000 INFO @ Tue, 14 Jul 2020 09:45:23: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 09:45:23: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 09:45:23: #1 total tags in treatment: 56551933 INFO @ Tue, 14 Jul 2020 09:45:23: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:45:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:45:24: #1 tags after filtering in treatment: 56551879 INFO @ Tue, 14 Jul 2020 09:45:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:45:24: #1 finished! INFO @ Tue, 14 Jul 2020 09:45:24: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:45:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:45:24: 51000000 INFO @ Tue, 14 Jul 2020 09:45:27: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 09:45:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:45:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX088868/ERX088868.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088868/ERX088868.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088868/ERX088868.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088868/ERX088868.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:45:29: 56000000 INFO @ Tue, 14 Jul 2020 09:45:30: 52000000 INFO @ Tue, 14 Jul 2020 09:45:32: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 09:45:32: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 09:45:32: #1 total tags in treatment: 56551933 INFO @ Tue, 14 Jul 2020 09:45:32: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:45:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:45:34: #1 tags after filtering in treatment: 56551879 INFO @ Tue, 14 Jul 2020 09:45:34: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:45:34: #1 finished! INFO @ Tue, 14 Jul 2020 09:45:34: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:45:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:45:36: 53000000 INFO @ Tue, 14 Jul 2020 09:45:37: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 09:45:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:45:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX088868/ERX088868.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088868/ERX088868.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088868/ERX088868.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088868/ERX088868.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:45:42: 54000000 INFO @ Tue, 14 Jul 2020 09:45:47: 55000000 INFO @ Tue, 14 Jul 2020 09:45:53: 56000000 INFO @ Tue, 14 Jul 2020 09:45:57: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 09:45:57: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 09:45:57: #1 total tags in treatment: 56551933 INFO @ Tue, 14 Jul 2020 09:45:57: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:45:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:45:58: #1 tags after filtering in treatment: 56551879 INFO @ Tue, 14 Jul 2020 09:45:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:45:58: #1 finished! INFO @ Tue, 14 Jul 2020 09:45:58: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:45:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:46:01: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 09:46:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:46:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX088868/ERX088868.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088868/ERX088868.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088868/ERX088868.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX088868/ERX088868.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling