Job ID = 6451753 SRX = ERX008197 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T07:16:17 prefetch.2.10.7: 1) Downloading 'ERR020071'... 2020-06-21T07:16:17 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T07:17:14 prefetch.2.10.7: HTTPS download succeed 2020-06-21T07:17:14 prefetch.2.10.7: 'ERR020071' is valid 2020-06-21T07:17:14 prefetch.2.10.7: 1) 'ERR020071' was downloaded successfully Read 3771193 spots for ERR020071/ERR020071.sra Written 3771193 spots for ERR020071/ERR020071.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:00:35 3771193 reads; of these: 3771193 (100.00%) were unpaired; of these: 26458 (0.70%) aligned 0 times 3405897 (90.31%) aligned exactly 1 time 338838 (8.98%) aligned >1 times 99.30% overall alignment rate Time searching: 00:00:36 Overall time: 00:00:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 207202 / 3744735 = 0.0553 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 16:19:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX008197/ERX008197.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX008197/ERX008197.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX008197/ERX008197.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX008197/ERX008197.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 16:19:36: #1 read tag files... INFO @ Sun, 21 Jun 2020 16:19:36: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 16:19:41: 1000000 INFO @ Sun, 21 Jun 2020 16:19:47: 2000000 INFO @ Sun, 21 Jun 2020 16:19:52: 3000000 INFO @ Sun, 21 Jun 2020 16:19:56: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 16:19:56: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 16:19:56: #1 total tags in treatment: 3537533 INFO @ Sun, 21 Jun 2020 16:19:56: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 16:19:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 16:19:56: #1 tags after filtering in treatment: 3537220 INFO @ Sun, 21 Jun 2020 16:19:56: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 16:19:56: #1 finished! INFO @ Sun, 21 Jun 2020 16:19:56: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 16:19:56: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 16:19:56: #2 number of paired peaks: 41 WARNING @ Sun, 21 Jun 2020 16:19:56: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 16:19:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX008197/ERX008197.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008197/ERX008197.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008197/ERX008197.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008197/ERX008197.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 16:20:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX008197/ERX008197.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX008197/ERX008197.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX008197/ERX008197.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX008197/ERX008197.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 16:20:06: #1 read tag files... INFO @ Sun, 21 Jun 2020 16:20:06: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 16:20:11: 1000000 INFO @ Sun, 21 Jun 2020 16:20:17: 2000000 INFO @ Sun, 21 Jun 2020 16:20:22: 3000000 INFO @ Sun, 21 Jun 2020 16:20:26: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 16:20:26: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 16:20:26: #1 total tags in treatment: 3537533 INFO @ Sun, 21 Jun 2020 16:20:26: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 16:20:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 16:20:26: #1 tags after filtering in treatment: 3537220 INFO @ Sun, 21 Jun 2020 16:20:26: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 16:20:26: #1 finished! INFO @ Sun, 21 Jun 2020 16:20:26: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 16:20:26: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 16:20:26: #2 number of paired peaks: 41 WARNING @ Sun, 21 Jun 2020 16:20:26: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 16:20:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX008197/ERX008197.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008197/ERX008197.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008197/ERX008197.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008197/ERX008197.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 16:20:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX008197/ERX008197.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX008197/ERX008197.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX008197/ERX008197.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX008197/ERX008197.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 16:20:36: #1 read tag files... INFO @ Sun, 21 Jun 2020 16:20:36: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 16:20:41: 1000000 INFO @ Sun, 21 Jun 2020 16:20:47: 2000000 INFO @ Sun, 21 Jun 2020 16:20:53: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 16:20:56: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 16:20:56: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 16:20:56: #1 total tags in treatment: 3537533 INFO @ Sun, 21 Jun 2020 16:20:56: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 16:20:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 16:20:56: #1 tags after filtering in treatment: 3537220 INFO @ Sun, 21 Jun 2020 16:20:56: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 16:20:56: #1 finished! INFO @ Sun, 21 Jun 2020 16:20:56: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 16:20:56: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 16:20:56: #2 number of paired peaks: 41 WARNING @ Sun, 21 Jun 2020 16:20:56: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 16:20:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX008197/ERX008197.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008197/ERX008197.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008197/ERX008197.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008197/ERX008197.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。