Job ID = 6451750 SRX = ERX008194 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T07:47:30 prefetch.2.10.7: 1) Downloading 'ERR020073'... 2020-06-21T07:47:30 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T07:51:07 prefetch.2.10.7: HTTPS download succeed 2020-06-21T07:51:08 prefetch.2.10.7: 'ERR020073' is valid 2020-06-21T07:51:08 prefetch.2.10.7: 1) 'ERR020073' was downloaded successfully Read 10254810 spots for ERR020073/ERR020073.sra Written 10254810 spots for ERR020073/ERR020073.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:31 10254810 reads; of these: 10254810 (100.00%) were unpaired; of these: 54797 (0.53%) aligned 0 times 9924951 (96.78%) aligned exactly 1 time 275062 (2.68%) aligned >1 times 99.47% overall alignment rate Time searching: 00:01:31 Overall time: 00:01:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 747854 / 10200013 = 0.0733 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 16:56:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX008194/ERX008194.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX008194/ERX008194.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX008194/ERX008194.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX008194/ERX008194.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 16:56:28: #1 read tag files... INFO @ Sun, 21 Jun 2020 16:56:28: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 16:56:34: 1000000 INFO @ Sun, 21 Jun 2020 16:56:39: 2000000 INFO @ Sun, 21 Jun 2020 16:56:45: 3000000 INFO @ Sun, 21 Jun 2020 16:56:49: 4000000 INFO @ Sun, 21 Jun 2020 16:56:54: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 16:56:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX008194/ERX008194.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX008194/ERX008194.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX008194/ERX008194.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX008194/ERX008194.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 16:56:58: #1 read tag files... INFO @ Sun, 21 Jun 2020 16:56:58: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 16:56:59: 6000000 INFO @ Sun, 21 Jun 2020 16:57:03: 1000000 INFO @ Sun, 21 Jun 2020 16:57:05: 7000000 INFO @ Sun, 21 Jun 2020 16:57:09: 2000000 INFO @ Sun, 21 Jun 2020 16:57:10: 8000000 INFO @ Sun, 21 Jun 2020 16:57:14: 3000000 INFO @ Sun, 21 Jun 2020 16:57:16: 9000000 INFO @ Sun, 21 Jun 2020 16:57:19: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 16:57:19: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 16:57:19: #1 total tags in treatment: 9452159 INFO @ Sun, 21 Jun 2020 16:57:19: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 16:57:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 16:57:19: #1 tags after filtering in treatment: 9451642 INFO @ Sun, 21 Jun 2020 16:57:19: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 16:57:19: #1 finished! INFO @ Sun, 21 Jun 2020 16:57:19: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 16:57:19: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 16:57:19: 4000000 INFO @ Sun, 21 Jun 2020 16:57:20: #2 number of paired peaks: 26 WARNING @ Sun, 21 Jun 2020 16:57:20: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 16:57:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX008194/ERX008194.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008194/ERX008194.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008194/ERX008194.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008194/ERX008194.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 16:57:24: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 16:57:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX008194/ERX008194.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX008194/ERX008194.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX008194/ERX008194.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX008194/ERX008194.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 16:57:28: #1 read tag files... INFO @ Sun, 21 Jun 2020 16:57:28: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 16:57:29: 6000000 INFO @ Sun, 21 Jun 2020 16:57:34: 7000000 INFO @ Sun, 21 Jun 2020 16:57:34: 1000000 INFO @ Sun, 21 Jun 2020 16:57:39: 8000000 INFO @ Sun, 21 Jun 2020 16:57:40: 2000000 INFO @ Sun, 21 Jun 2020 16:57:45: 9000000 INFO @ Sun, 21 Jun 2020 16:57:46: 3000000 INFO @ Sun, 21 Jun 2020 16:57:48: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 16:57:48: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 16:57:48: #1 total tags in treatment: 9452159 INFO @ Sun, 21 Jun 2020 16:57:48: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 16:57:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 16:57:48: #1 tags after filtering in treatment: 9451642 INFO @ Sun, 21 Jun 2020 16:57:48: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 16:57:48: #1 finished! INFO @ Sun, 21 Jun 2020 16:57:48: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 16:57:48: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 16:57:49: #2 number of paired peaks: 26 WARNING @ Sun, 21 Jun 2020 16:57:49: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 16:57:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX008194/ERX008194.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008194/ERX008194.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008194/ERX008194.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008194/ERX008194.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 16:57:52: 4000000 INFO @ Sun, 21 Jun 2020 16:57:57: 5000000 INFO @ Sun, 21 Jun 2020 16:58:02: 6000000 INFO @ Sun, 21 Jun 2020 16:58:07: 7000000 INFO @ Sun, 21 Jun 2020 16:58:12: 8000000 INFO @ Sun, 21 Jun 2020 16:58:18: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 16:58:20: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 16:58:20: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 16:58:20: #1 total tags in treatment: 9452159 INFO @ Sun, 21 Jun 2020 16:58:20: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 16:58:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 16:58:21: #1 tags after filtering in treatment: 9451642 INFO @ Sun, 21 Jun 2020 16:58:21: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 16:58:21: #1 finished! INFO @ Sun, 21 Jun 2020 16:58:21: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 16:58:21: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 16:58:22: #2 number of paired peaks: 26 WARNING @ Sun, 21 Jun 2020 16:58:22: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 16:58:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX008194/ERX008194.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008194/ERX008194.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008194/ERX008194.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008194/ERX008194.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。