Job ID = 6451744 SRX = ERX008180 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T07:31:26 prefetch.2.10.7: 1) Downloading 'ERR020072'... 2020-06-21T07:31:26 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T07:33:54 prefetch.2.10.7: HTTPS download succeed 2020-06-21T07:33:54 prefetch.2.10.7: 'ERR020072' is valid 2020-06-21T07:33:54 prefetch.2.10.7: 1) 'ERR020072' was downloaded successfully Read 8809280 spots for ERR020072/ERR020072.sra Written 8809280 spots for ERR020072/ERR020072.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:22 8809280 reads; of these: 8809280 (100.00%) were unpaired; of these: 44120 (0.50%) aligned 0 times 8585354 (97.46%) aligned exactly 1 time 179806 (2.04%) aligned >1 times 99.50% overall alignment rate Time searching: 00:01:22 Overall time: 00:01:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 363239 / 8765160 = 0.0414 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 16:38:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX008180/ERX008180.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX008180/ERX008180.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX008180/ERX008180.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX008180/ERX008180.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 16:38:16: #1 read tag files... INFO @ Sun, 21 Jun 2020 16:38:16: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 16:38:22: 1000000 INFO @ Sun, 21 Jun 2020 16:38:28: 2000000 INFO @ Sun, 21 Jun 2020 16:38:34: 3000000 INFO @ Sun, 21 Jun 2020 16:38:40: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 16:38:45: 5000000 INFO @ Sun, 21 Jun 2020 16:38:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX008180/ERX008180.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX008180/ERX008180.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX008180/ERX008180.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX008180/ERX008180.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 16:38:46: #1 read tag files... INFO @ Sun, 21 Jun 2020 16:38:46: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 16:38:51: 6000000 INFO @ Sun, 21 Jun 2020 16:38:52: 1000000 INFO @ Sun, 21 Jun 2020 16:38:58: 7000000 INFO @ Sun, 21 Jun 2020 16:38:58: 2000000 INFO @ Sun, 21 Jun 2020 16:39:04: 8000000 INFO @ Sun, 21 Jun 2020 16:39:04: 3000000 INFO @ Sun, 21 Jun 2020 16:39:07: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 16:39:07: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 16:39:07: #1 total tags in treatment: 8401921 INFO @ Sun, 21 Jun 2020 16:39:07: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 16:39:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 16:39:07: #1 tags after filtering in treatment: 8401159 INFO @ Sun, 21 Jun 2020 16:39:07: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 16:39:07: #1 finished! INFO @ Sun, 21 Jun 2020 16:39:07: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 16:39:07: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 16:39:07: #2 number of paired peaks: 27 WARNING @ Sun, 21 Jun 2020 16:39:07: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 16:39:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX008180/ERX008180.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008180/ERX008180.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008180/ERX008180.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008180/ERX008180.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 16:39:10: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 16:39:16: 5000000 INFO @ Sun, 21 Jun 2020 16:39:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX008180/ERX008180.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX008180/ERX008180.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX008180/ERX008180.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX008180/ERX008180.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 16:39:16: #1 read tag files... INFO @ Sun, 21 Jun 2020 16:39:16: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 16:39:22: 1000000 INFO @ Sun, 21 Jun 2020 16:39:22: 6000000 INFO @ Sun, 21 Jun 2020 16:39:28: 2000000 INFO @ Sun, 21 Jun 2020 16:39:28: 7000000 INFO @ Sun, 21 Jun 2020 16:39:34: 3000000 INFO @ Sun, 21 Jun 2020 16:39:35: 8000000 INFO @ Sun, 21 Jun 2020 16:39:37: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 16:39:37: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 16:39:37: #1 total tags in treatment: 8401921 INFO @ Sun, 21 Jun 2020 16:39:37: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 16:39:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 16:39:37: #1 tags after filtering in treatment: 8401159 INFO @ Sun, 21 Jun 2020 16:39:37: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 16:39:37: #1 finished! INFO @ Sun, 21 Jun 2020 16:39:37: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 16:39:37: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 16:39:38: #2 number of paired peaks: 27 WARNING @ Sun, 21 Jun 2020 16:39:38: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 16:39:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX008180/ERX008180.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008180/ERX008180.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008180/ERX008180.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008180/ERX008180.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 16:39:40: 4000000 INFO @ Sun, 21 Jun 2020 16:39:46: 5000000 INFO @ Sun, 21 Jun 2020 16:39:52: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 16:39:57: 7000000 INFO @ Sun, 21 Jun 2020 16:40:04: 8000000 INFO @ Sun, 21 Jun 2020 16:40:06: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 16:40:06: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 16:40:06: #1 total tags in treatment: 8401921 INFO @ Sun, 21 Jun 2020 16:40:06: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 16:40:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 16:40:06: #1 tags after filtering in treatment: 8401159 INFO @ Sun, 21 Jun 2020 16:40:06: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 16:40:06: #1 finished! INFO @ Sun, 21 Jun 2020 16:40:06: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 16:40:06: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 16:40:07: #2 number of paired peaks: 27 WARNING @ Sun, 21 Jun 2020 16:40:07: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 16:40:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX008180/ERX008180.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008180/ERX008180.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008180/ERX008180.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008180/ERX008180.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。