Job ID = 6451743 SRX = ERX008178 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T07:22:32 prefetch.2.10.7: 1) Downloading 'ERR020070'... 2020-06-21T07:22:32 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T07:23:06 prefetch.2.10.7: HTTPS download succeed 2020-06-21T07:23:06 prefetch.2.10.7: 'ERR020070' is valid 2020-06-21T07:23:06 prefetch.2.10.7: 1) 'ERR020070' was downloaded successfully Read 2710538 spots for ERR020070/ERR020070.sra Written 2710538 spots for ERR020070/ERR020070.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:25 2710538 reads; of these: 2710538 (100.00%) were unpaired; of these: 19947 (0.74%) aligned 0 times 2440800 (90.05%) aligned exactly 1 time 249791 (9.22%) aligned >1 times 99.26% overall alignment rate Time searching: 00:00:26 Overall time: 00:00:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 86096 / 2690591 = 0.0320 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 16:24:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX008178/ERX008178.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX008178/ERX008178.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX008178/ERX008178.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX008178/ERX008178.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 16:24:48: #1 read tag files... INFO @ Sun, 21 Jun 2020 16:24:48: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 16:24:53: 1000000 INFO @ Sun, 21 Jun 2020 16:24:58: 2000000 INFO @ Sun, 21 Jun 2020 16:25:02: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 16:25:02: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 16:25:02: #1 total tags in treatment: 2604495 INFO @ Sun, 21 Jun 2020 16:25:02: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 16:25:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 16:25:02: #1 tags after filtering in treatment: 2604011 INFO @ Sun, 21 Jun 2020 16:25:02: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 16:25:02: #1 finished! INFO @ Sun, 21 Jun 2020 16:25:02: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 16:25:02: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 16:25:03: #2 number of paired peaks: 32 WARNING @ Sun, 21 Jun 2020 16:25:03: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 16:25:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX008178/ERX008178.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008178/ERX008178.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008178/ERX008178.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008178/ERX008178.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 16:25:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX008178/ERX008178.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX008178/ERX008178.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX008178/ERX008178.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX008178/ERX008178.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 16:25:18: #1 read tag files... INFO @ Sun, 21 Jun 2020 16:25:18: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 16:25:23: 1000000 INFO @ Sun, 21 Jun 2020 16:25:28: 2000000 INFO @ Sun, 21 Jun 2020 16:25:32: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 16:25:32: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 16:25:32: #1 total tags in treatment: 2604495 INFO @ Sun, 21 Jun 2020 16:25:32: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 16:25:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 16:25:32: #1 tags after filtering in treatment: 2604011 INFO @ Sun, 21 Jun 2020 16:25:32: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 16:25:32: #1 finished! INFO @ Sun, 21 Jun 2020 16:25:32: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 16:25:32: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 16:25:33: #2 number of paired peaks: 32 WARNING @ Sun, 21 Jun 2020 16:25:33: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 16:25:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX008178/ERX008178.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008178/ERX008178.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008178/ERX008178.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008178/ERX008178.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 16:25:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/ERX008178/ERX008178.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/ERX008178/ERX008178.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/ERX008178/ERX008178.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/ERX008178/ERX008178.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 16:25:48: #1 read tag files... INFO @ Sun, 21 Jun 2020 16:25:48: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 16:25:54: 1000000 INFO @ Sun, 21 Jun 2020 16:25:59: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 16:26:03: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 16:26:03: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 16:26:03: #1 total tags in treatment: 2604495 INFO @ Sun, 21 Jun 2020 16:26:03: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 16:26:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 16:26:04: #1 tags after filtering in treatment: 2604011 INFO @ Sun, 21 Jun 2020 16:26:04: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 16:26:04: #1 finished! INFO @ Sun, 21 Jun 2020 16:26:04: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 16:26:04: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 16:26:04: #2 number of paired peaks: 32 WARNING @ Sun, 21 Jun 2020 16:26:04: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 16:26:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/ERX008178/ERX008178.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008178/ERX008178.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008178/ERX008178.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/ERX008178/ERX008178.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。