SRX330995	SAMN02299536	GSM1200247:_S2_DsxF;_Drosophila_melanogaster;_ChIP-Seq	ChIP-Seq	GENOMIC	ChIP	xxx	xxx	xxx	11142413	Illumina_Genome_Analyzer	SRA096569	SRS466576	SRP028410	PRJNA214088	2014-11-03T13:57:08Z	Organism taxonomy_id="7227" taxonomy_name="Drosophila melanogaster"	source_name=S2 cells transfected with pMT5.1- DSXF -V5-His B	chip antibody=anti-V5 (Invitrogen)	cell line=S2	treatment=Transfection of S2 cells was performed using Effectene Transfection Reagent (Qiagen, Valencia, CA). pCoBlast (Invitrogen, Carlsbad, CA) was used as the selection plasmid. For the transfection procedure, cells were diluted to a density of 10^6 cells/ml and grew afterwards for about 20 hours. 1 µg expression plasmid, 50 ng pCoBlast, Effectene Transfection components and 840 µl complete Schneider’s Drosophila medium were mixed according to the manufacturer’s protocol and immediately added drop-wise onto the cells. The cells were subsequently grown in complete Schneider’s Drosophila medium for 60 hours prior to selection with 30 µg/ml blasticidin (Invitrogen, Carlsbad, CA). After 5 weeks of selection, the blasticidin-resistant cells were maintained in complete Schneider’s Drosophila medium containing 25 µg/ml of blasticidin. Expression of the recombinant proteins from the MT promoter was induced by adding copper sulfate to the medium to a final concentration of 500 μM. Before induction of expression cells were seeded to a density of 1*106 cells/ ml. Expression of the recombinant proteins was induced at a cell density of 3*10^6 cells/ ml. 60 ml of induced transfected Drosophila S2 cells expressing the recombinant proteins DSXM -V5-His or DSXF -V5-His respectively were grown in cell culture bottles for 36 hours reaching a cell density of 4.5*10^6 cells per ml.	amount of dna used to make library=100 ng