Job ID = 14171407 SRX = SRX9986172 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 19835022 spots for SRR13591526/SRR13591526.sra Written 19835022 spots for SRR13591526/SRR13591526.sra fastq に変換しました。 bowtie でマッピング中... Your job 14172005 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:26:09 19835022 reads; of these: 19835022 (100.00%) were paired; of these: 3142038 (15.84%) aligned concordantly 0 times 13627162 (68.70%) aligned concordantly exactly 1 time 3065822 (15.46%) aligned concordantly >1 times ---- 3142038 pairs aligned concordantly 0 times; of these: 930279 (29.61%) aligned discordantly 1 time ---- 2211759 pairs aligned 0 times concordantly or discordantly; of these: 4423518 mates make up the pairs; of these: 2828274 (63.94%) aligned 0 times 841689 (19.03%) aligned exactly 1 time 753555 (17.04%) aligned >1 times 92.87% overall alignment rate Time searching: 00:26:09 Overall time: 00:26:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 930815 / 17539273 = 0.0531 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:10:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986172/SRX9986172.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986172/SRX9986172.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986172/SRX9986172.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986172/SRX9986172.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:10:21: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:10:21: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:10:26: 1000000 INFO @ Sat, 11 Dec 2021 12:10:31: 2000000 INFO @ Sat, 11 Dec 2021 12:10:35: 3000000 INFO @ Sat, 11 Dec 2021 12:10:40: 4000000 INFO @ Sat, 11 Dec 2021 12:10:45: 5000000 INFO @ Sat, 11 Dec 2021 12:10:49: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:10:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986172/SRX9986172.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986172/SRX9986172.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986172/SRX9986172.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986172/SRX9986172.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:10:51: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:10:51: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:10:54: 7000000 INFO @ Sat, 11 Dec 2021 12:10:57: 1000000 INFO @ Sat, 11 Dec 2021 12:10:59: 8000000 INFO @ Sat, 11 Dec 2021 12:11:02: 2000000 INFO @ Sat, 11 Dec 2021 12:11:04: 9000000 INFO @ Sat, 11 Dec 2021 12:11:07: 3000000 INFO @ Sat, 11 Dec 2021 12:11:09: 10000000 INFO @ Sat, 11 Dec 2021 12:11:11: 4000000 INFO @ Sat, 11 Dec 2021 12:11:13: 11000000 INFO @ Sat, 11 Dec 2021 12:11:16: 5000000 INFO @ Sat, 11 Dec 2021 12:11:18: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:11:21: 6000000 INFO @ Sat, 11 Dec 2021 12:11:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986172/SRX9986172.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986172/SRX9986172.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986172/SRX9986172.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986172/SRX9986172.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:11:21: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:11:21: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:11:23: 13000000 INFO @ Sat, 11 Dec 2021 12:11:26: 7000000 INFO @ Sat, 11 Dec 2021 12:11:27: 1000000 INFO @ Sat, 11 Dec 2021 12:11:28: 14000000 INFO @ Sat, 11 Dec 2021 12:11:31: 8000000 INFO @ Sat, 11 Dec 2021 12:11:32: 2000000 INFO @ Sat, 11 Dec 2021 12:11:33: 15000000 INFO @ Sat, 11 Dec 2021 12:11:36: 9000000 INFO @ Sat, 11 Dec 2021 12:11:37: 3000000 INFO @ Sat, 11 Dec 2021 12:11:38: 16000000 INFO @ Sat, 11 Dec 2021 12:11:41: 10000000 INFO @ Sat, 11 Dec 2021 12:11:42: 4000000 INFO @ Sat, 11 Dec 2021 12:11:43: 17000000 INFO @ Sat, 11 Dec 2021 12:11:46: 11000000 INFO @ Sat, 11 Dec 2021 12:11:47: 5000000 INFO @ Sat, 11 Dec 2021 12:11:48: 18000000 INFO @ Sat, 11 Dec 2021 12:11:51: 12000000 INFO @ Sat, 11 Dec 2021 12:11:52: 6000000 INFO @ Sat, 11 Dec 2021 12:11:53: 19000000 INFO @ Sat, 11 Dec 2021 12:11:56: 13000000 INFO @ Sat, 11 Dec 2021 12:11:57: 7000000 INFO @ Sat, 11 Dec 2021 12:11:58: 20000000 INFO @ Sat, 11 Dec 2021 12:12:01: 14000000 INFO @ Sat, 11 Dec 2021 12:12:02: 8000000 INFO @ Sat, 11 Dec 2021 12:12:03: 21000000 INFO @ Sat, 11 Dec 2021 12:12:06: 15000000 INFO @ Sat, 11 Dec 2021 12:12:07: 9000000 INFO @ Sat, 11 Dec 2021 12:12:08: 22000000 INFO @ Sat, 11 Dec 2021 12:12:11: 16000000 INFO @ Sat, 11 Dec 2021 12:12:12: 10000000 INFO @ Sat, 11 Dec 2021 12:12:13: 23000000 INFO @ Sat, 11 Dec 2021 12:12:16: 17000000 INFO @ Sat, 11 Dec 2021 12:12:16: 11000000 INFO @ Sat, 11 Dec 2021 12:12:18: 24000000 INFO @ Sat, 11 Dec 2021 12:12:21: 18000000 INFO @ Sat, 11 Dec 2021 12:12:21: 12000000 INFO @ Sat, 11 Dec 2021 12:12:23: 25000000 INFO @ Sat, 11 Dec 2021 12:12:26: 19000000 INFO @ Sat, 11 Dec 2021 12:12:26: 13000000 INFO @ Sat, 11 Dec 2021 12:12:28: 26000000 INFO @ Sat, 11 Dec 2021 12:12:30: 20000000 INFO @ Sat, 11 Dec 2021 12:12:31: 14000000 INFO @ Sat, 11 Dec 2021 12:12:33: 27000000 INFO @ Sat, 11 Dec 2021 12:12:35: 21000000 INFO @ Sat, 11 Dec 2021 12:12:36: 15000000 INFO @ Sat, 11 Dec 2021 12:12:38: 28000000 INFO @ Sat, 11 Dec 2021 12:12:40: 22000000 INFO @ Sat, 11 Dec 2021 12:12:41: 16000000 INFO @ Sat, 11 Dec 2021 12:12:43: 29000000 INFO @ Sat, 11 Dec 2021 12:12:45: 23000000 INFO @ Sat, 11 Dec 2021 12:12:46: 17000000 INFO @ Sat, 11 Dec 2021 12:12:48: 30000000 INFO @ Sat, 11 Dec 2021 12:12:50: 24000000 INFO @ Sat, 11 Dec 2021 12:12:51: 18000000 INFO @ Sat, 11 Dec 2021 12:12:53: 31000000 INFO @ Sat, 11 Dec 2021 12:12:55: 25000000 INFO @ Sat, 11 Dec 2021 12:12:56: 19000000 INFO @ Sat, 11 Dec 2021 12:12:58: 32000000 INFO @ Sat, 11 Dec 2021 12:13:00: 26000000 INFO @ Sat, 11 Dec 2021 12:13:01: 20000000 INFO @ Sat, 11 Dec 2021 12:13:03: 33000000 INFO @ Sat, 11 Dec 2021 12:13:05: 27000000 INFO @ Sat, 11 Dec 2021 12:13:06: 21000000 INFO @ Sat, 11 Dec 2021 12:13:08: 34000000 INFO @ Sat, 11 Dec 2021 12:13:10: 28000000 INFO @ Sat, 11 Dec 2021 12:13:11: 22000000 INFO @ Sat, 11 Dec 2021 12:13:13: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 12:13:13: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 12:13:13: #1 total tags in treatment: 15771401 INFO @ Sat, 11 Dec 2021 12:13:13: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:13:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:13:13: #1 tags after filtering in treatment: 13133886 INFO @ Sat, 11 Dec 2021 12:13:13: #1 Redundant rate of treatment: 0.17 INFO @ Sat, 11 Dec 2021 12:13:13: #1 finished! INFO @ Sat, 11 Dec 2021 12:13:13: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:13:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:13:14: #2 number of paired peaks: 50 WARNING @ Sat, 11 Dec 2021 12:13:14: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:13:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986172/SRX9986172.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986172/SRX9986172.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986172/SRX9986172.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986172/SRX9986172.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 12:13:15: 29000000 INFO @ Sat, 11 Dec 2021 12:13:16: 23000000 INFO @ Sat, 11 Dec 2021 12:13:20: 30000000 INFO @ Sat, 11 Dec 2021 12:13:21: 24000000 INFO @ Sat, 11 Dec 2021 12:13:25: 31000000 INFO @ Sat, 11 Dec 2021 12:13:26: 25000000 INFO @ Sat, 11 Dec 2021 12:13:30: 32000000 INFO @ Sat, 11 Dec 2021 12:13:31: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 12:13:35: 33000000 INFO @ Sat, 11 Dec 2021 12:13:36: 27000000 INFO @ Sat, 11 Dec 2021 12:13:40: 34000000 INFO @ Sat, 11 Dec 2021 12:13:41: 28000000 INFO @ Sat, 11 Dec 2021 12:13:45: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 12:13:45: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 12:13:45: #1 total tags in treatment: 15771401 INFO @ Sat, 11 Dec 2021 12:13:45: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:13:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:13:45: #1 tags after filtering in treatment: 13133886 INFO @ Sat, 11 Dec 2021 12:13:45: #1 Redundant rate of treatment: 0.17 INFO @ Sat, 11 Dec 2021 12:13:45: #1 finished! INFO @ Sat, 11 Dec 2021 12:13:45: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:13:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:13:46: 29000000 INFO @ Sat, 11 Dec 2021 12:13:46: #2 number of paired peaks: 50 WARNING @ Sat, 11 Dec 2021 12:13:46: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:13:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986172/SRX9986172.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986172/SRX9986172.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986172/SRX9986172.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986172/SRX9986172.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 12:13:51: 30000000 INFO @ Sat, 11 Dec 2021 12:13:56: 31000000 INFO @ Sat, 11 Dec 2021 12:14:00: 32000000 INFO @ Sat, 11 Dec 2021 12:14:05: 33000000 INFO @ Sat, 11 Dec 2021 12:14:10: 34000000 INFO @ Sat, 11 Dec 2021 12:14:15: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 12:14:15: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 12:14:15: #1 total tags in treatment: 15771401 INFO @ Sat, 11 Dec 2021 12:14:15: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:14:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:14:15: #1 tags after filtering in treatment: 13133886 INFO @ Sat, 11 Dec 2021 12:14:15: #1 Redundant rate of treatment: 0.17 INFO @ Sat, 11 Dec 2021 12:14:15: #1 finished! INFO @ Sat, 11 Dec 2021 12:14:15: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:14:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:14:16: #2 number of paired peaks: 50 WARNING @ Sat, 11 Dec 2021 12:14:16: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:14:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986172/SRX9986172.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986172/SRX9986172.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986172/SRX9986172.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986172/SRX9986172.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。