Job ID = 14171378 SRX = SRX9986169 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 30598135 spots for SRR13591523/SRR13591523.sra Written 30598135 spots for SRR13591523/SRR13591523.sra fastq に変換しました。 bowtie でマッピング中... Your job 14172046 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:43:25 30598135 reads; of these: 30598135 (100.00%) were paired; of these: 4807671 (15.71%) aligned concordantly 0 times 19609925 (64.09%) aligned concordantly exactly 1 time 6180539 (20.20%) aligned concordantly >1 times ---- 4807671 pairs aligned concordantly 0 times; of these: 409227 (8.51%) aligned discordantly 1 time ---- 4398444 pairs aligned 0 times concordantly or discordantly; of these: 8796888 mates make up the pairs; of these: 7811433 (88.80%) aligned 0 times 635424 (7.22%) aligned exactly 1 time 350031 (3.98%) aligned >1 times 87.24% overall alignment rate Time searching: 00:43:25 Overall time: 00:43:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 1645458 / 26105383 = 0.0630 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:26:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986169/SRX9986169.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986169/SRX9986169.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986169/SRX9986169.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986169/SRX9986169.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:26:18: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:26:18: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:26:23: 1000000 INFO @ Sat, 11 Dec 2021 12:26:28: 2000000 INFO @ Sat, 11 Dec 2021 12:26:33: 3000000 INFO @ Sat, 11 Dec 2021 12:26:38: 4000000 INFO @ Sat, 11 Dec 2021 12:26:43: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:26:48: 6000000 INFO @ Sat, 11 Dec 2021 12:26:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986169/SRX9986169.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986169/SRX9986169.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986169/SRX9986169.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986169/SRX9986169.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:26:48: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:26:48: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:26:54: 7000000 INFO @ Sat, 11 Dec 2021 12:26:55: 1000000 INFO @ Sat, 11 Dec 2021 12:27:00: 8000000 INFO @ Sat, 11 Dec 2021 12:27:02: 2000000 INFO @ Sat, 11 Dec 2021 12:27:06: 9000000 INFO @ Sat, 11 Dec 2021 12:27:09: 3000000 INFO @ Sat, 11 Dec 2021 12:27:12: 10000000 INFO @ Sat, 11 Dec 2021 12:27:16: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:27:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986169/SRX9986169.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986169/SRX9986169.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986169/SRX9986169.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986169/SRX9986169.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:27:18: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:27:18: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:27:18: 11000000 INFO @ Sat, 11 Dec 2021 12:27:22: 5000000 INFO @ Sat, 11 Dec 2021 12:27:24: 12000000 INFO @ Sat, 11 Dec 2021 12:27:25: 1000000 INFO @ Sat, 11 Dec 2021 12:27:29: 6000000 INFO @ Sat, 11 Dec 2021 12:27:31: 13000000 INFO @ Sat, 11 Dec 2021 12:27:32: 2000000 INFO @ Sat, 11 Dec 2021 12:27:36: 7000000 INFO @ Sat, 11 Dec 2021 12:27:37: 14000000 INFO @ Sat, 11 Dec 2021 12:27:39: 3000000 INFO @ Sat, 11 Dec 2021 12:27:43: 15000000 INFO @ Sat, 11 Dec 2021 12:27:43: 8000000 INFO @ Sat, 11 Dec 2021 12:27:46: 4000000 INFO @ Sat, 11 Dec 2021 12:27:49: 16000000 INFO @ Sat, 11 Dec 2021 12:27:50: 9000000 INFO @ Sat, 11 Dec 2021 12:27:53: 5000000 INFO @ Sat, 11 Dec 2021 12:27:55: 17000000 INFO @ Sat, 11 Dec 2021 12:27:57: 10000000 INFO @ Sat, 11 Dec 2021 12:28:00: 6000000 INFO @ Sat, 11 Dec 2021 12:28:02: 18000000 INFO @ Sat, 11 Dec 2021 12:28:04: 11000000 INFO @ Sat, 11 Dec 2021 12:28:07: 7000000 INFO @ Sat, 11 Dec 2021 12:28:08: 19000000 INFO @ Sat, 11 Dec 2021 12:28:11: 12000000 INFO @ Sat, 11 Dec 2021 12:28:14: 8000000 INFO @ Sat, 11 Dec 2021 12:28:14: 20000000 INFO @ Sat, 11 Dec 2021 12:28:18: 13000000 INFO @ Sat, 11 Dec 2021 12:28:20: 21000000 INFO @ Sat, 11 Dec 2021 12:28:21: 9000000 INFO @ Sat, 11 Dec 2021 12:28:25: 14000000 INFO @ Sat, 11 Dec 2021 12:28:26: 22000000 INFO @ Sat, 11 Dec 2021 12:28:28: 10000000 INFO @ Sat, 11 Dec 2021 12:28:32: 15000000 INFO @ Sat, 11 Dec 2021 12:28:32: 23000000 INFO @ Sat, 11 Dec 2021 12:28:35: 11000000 INFO @ Sat, 11 Dec 2021 12:28:39: 24000000 INFO @ Sat, 11 Dec 2021 12:28:39: 16000000 INFO @ Sat, 11 Dec 2021 12:28:42: 12000000 INFO @ Sat, 11 Dec 2021 12:28:45: 25000000 INFO @ Sat, 11 Dec 2021 12:28:46: 17000000 INFO @ Sat, 11 Dec 2021 12:28:49: 13000000 INFO @ Sat, 11 Dec 2021 12:28:51: 26000000 INFO @ Sat, 11 Dec 2021 12:28:52: 18000000 INFO @ Sat, 11 Dec 2021 12:28:56: 14000000 INFO @ Sat, 11 Dec 2021 12:28:57: 27000000 INFO @ Sat, 11 Dec 2021 12:28:59: 19000000 INFO @ Sat, 11 Dec 2021 12:29:03: 15000000 INFO @ Sat, 11 Dec 2021 12:29:03: 28000000 INFO @ Sat, 11 Dec 2021 12:29:06: 20000000 INFO @ Sat, 11 Dec 2021 12:29:09: 29000000 INFO @ Sat, 11 Dec 2021 12:29:10: 16000000 INFO @ Sat, 11 Dec 2021 12:29:13: 21000000 INFO @ Sat, 11 Dec 2021 12:29:15: 30000000 INFO @ Sat, 11 Dec 2021 12:29:17: 17000000 INFO @ Sat, 11 Dec 2021 12:29:20: 22000000 INFO @ Sat, 11 Dec 2021 12:29:22: 31000000 INFO @ Sat, 11 Dec 2021 12:29:24: 18000000 INFO @ Sat, 11 Dec 2021 12:29:27: 23000000 INFO @ Sat, 11 Dec 2021 12:29:28: 32000000 INFO @ Sat, 11 Dec 2021 12:29:31: 19000000 INFO @ Sat, 11 Dec 2021 12:29:33: 24000000 INFO @ Sat, 11 Dec 2021 12:29:34: 33000000 INFO @ Sat, 11 Dec 2021 12:29:38: 20000000 INFO @ Sat, 11 Dec 2021 12:29:40: 25000000 INFO @ Sat, 11 Dec 2021 12:29:40: 34000000 INFO @ Sat, 11 Dec 2021 12:29:45: 21000000 INFO @ Sat, 11 Dec 2021 12:29:46: 35000000 INFO @ Sat, 11 Dec 2021 12:29:47: 26000000 INFO @ Sat, 11 Dec 2021 12:29:52: 22000000 INFO @ Sat, 11 Dec 2021 12:29:53: 36000000 INFO @ Sat, 11 Dec 2021 12:29:53: 27000000 INFO @ Sat, 11 Dec 2021 12:29:59: 37000000 INFO @ Sat, 11 Dec 2021 12:29:59: 23000000 INFO @ Sat, 11 Dec 2021 12:30:00: 28000000 INFO @ Sat, 11 Dec 2021 12:30:05: 38000000 INFO @ Sat, 11 Dec 2021 12:30:06: 24000000 INFO @ Sat, 11 Dec 2021 12:30:07: 29000000 INFO @ Sat, 11 Dec 2021 12:30:11: 39000000 INFO @ Sat, 11 Dec 2021 12:30:13: 25000000 INFO @ Sat, 11 Dec 2021 12:30:14: 30000000 INFO @ Sat, 11 Dec 2021 12:30:18: 40000000 INFO @ Sat, 11 Dec 2021 12:30:20: 26000000 INFO @ Sat, 11 Dec 2021 12:30:20: 31000000 INFO @ Sat, 11 Dec 2021 12:30:24: 41000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 12:30:27: 32000000 INFO @ Sat, 11 Dec 2021 12:30:27: 27000000 INFO @ Sat, 11 Dec 2021 12:30:30: 42000000 INFO @ Sat, 11 Dec 2021 12:30:34: 33000000 INFO @ Sat, 11 Dec 2021 12:30:34: 28000000 INFO @ Sat, 11 Dec 2021 12:30:36: 43000000 INFO @ Sat, 11 Dec 2021 12:30:40: 34000000 INFO @ Sat, 11 Dec 2021 12:30:41: 29000000 INFO @ Sat, 11 Dec 2021 12:30:42: 44000000 INFO @ Sat, 11 Dec 2021 12:30:47: 35000000 INFO @ Sat, 11 Dec 2021 12:30:48: 30000000 INFO @ Sat, 11 Dec 2021 12:30:49: 45000000 INFO @ Sat, 11 Dec 2021 12:30:54: 36000000 INFO @ Sat, 11 Dec 2021 12:30:55: 46000000 INFO @ Sat, 11 Dec 2021 12:30:55: 31000000 INFO @ Sat, 11 Dec 2021 12:31:00: 37000000 INFO @ Sat, 11 Dec 2021 12:31:01: 47000000 INFO @ Sat, 11 Dec 2021 12:31:02: 32000000 INFO @ Sat, 11 Dec 2021 12:31:07: 48000000 INFO @ Sat, 11 Dec 2021 12:31:07: 38000000 INFO @ Sat, 11 Dec 2021 12:31:09: 33000000 INFO @ Sat, 11 Dec 2021 12:31:13: 49000000 INFO @ Sat, 11 Dec 2021 12:31:14: 39000000 INFO @ Sat, 11 Dec 2021 12:31:16: 34000000 INFO @ Sat, 11 Dec 2021 12:31:19: 50000000 INFO @ Sat, 11 Dec 2021 12:31:20: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 12:31:20: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 12:31:20: #1 total tags in treatment: 24156501 INFO @ Sat, 11 Dec 2021 12:31:20: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:31:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:31:21: 40000000 INFO @ Sat, 11 Dec 2021 12:31:21: #1 tags after filtering in treatment: 19198308 INFO @ Sat, 11 Dec 2021 12:31:21: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 11 Dec 2021 12:31:21: #1 finished! INFO @ Sat, 11 Dec 2021 12:31:21: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:31:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:31:22: #2 number of paired peaks: 22 WARNING @ Sat, 11 Dec 2021 12:31:22: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:31:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986169/SRX9986169.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986169/SRX9986169.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986169/SRX9986169.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986169/SRX9986169.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 12:31:23: 35000000 INFO @ Sat, 11 Dec 2021 12:31:27: 41000000 INFO @ Sat, 11 Dec 2021 12:31:30: 36000000 INFO @ Sat, 11 Dec 2021 12:31:34: 42000000 INFO @ Sat, 11 Dec 2021 12:31:37: 37000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 12:31:41: 43000000 INFO @ Sat, 11 Dec 2021 12:31:44: 38000000 INFO @ Sat, 11 Dec 2021 12:31:47: 44000000 INFO @ Sat, 11 Dec 2021 12:31:51: 39000000 INFO @ Sat, 11 Dec 2021 12:31:54: 45000000 INFO @ Sat, 11 Dec 2021 12:31:58: 40000000 INFO @ Sat, 11 Dec 2021 12:32:00: 46000000 INFO @ Sat, 11 Dec 2021 12:32:05: 41000000 INFO @ Sat, 11 Dec 2021 12:32:07: 47000000 INFO @ Sat, 11 Dec 2021 12:32:12: 42000000 INFO @ Sat, 11 Dec 2021 12:32:13: 48000000 INFO @ Sat, 11 Dec 2021 12:32:18: 43000000 INFO @ Sat, 11 Dec 2021 12:32:20: 49000000 INFO @ Sat, 11 Dec 2021 12:32:25: 44000000 INFO @ Sat, 11 Dec 2021 12:32:27: 50000000 INFO @ Sat, 11 Dec 2021 12:32:27: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 12:32:27: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 12:32:27: #1 total tags in treatment: 24156501 INFO @ Sat, 11 Dec 2021 12:32:27: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:32:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:32:28: #1 tags after filtering in treatment: 19198308 INFO @ Sat, 11 Dec 2021 12:32:28: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 11 Dec 2021 12:32:28: #1 finished! INFO @ Sat, 11 Dec 2021 12:32:28: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:32:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:32:29: #2 number of paired peaks: 22 WARNING @ Sat, 11 Dec 2021 12:32:29: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:32:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986169/SRX9986169.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986169/SRX9986169.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986169/SRX9986169.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986169/SRX9986169.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 12:32:31: 45000000 INFO @ Sat, 11 Dec 2021 12:32:38: 46000000 INFO @ Sat, 11 Dec 2021 12:32:44: 47000000 INFO @ Sat, 11 Dec 2021 12:32:50: 48000000 INFO @ Sat, 11 Dec 2021 12:32:56: 49000000 INFO @ Sat, 11 Dec 2021 12:33:03: 50000000 INFO @ Sat, 11 Dec 2021 12:33:03: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 12:33:03: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 12:33:03: #1 total tags in treatment: 24156501 INFO @ Sat, 11 Dec 2021 12:33:03: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:33:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:33:04: #1 tags after filtering in treatment: 19198308 INFO @ Sat, 11 Dec 2021 12:33:04: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 11 Dec 2021 12:33:04: #1 finished! INFO @ Sat, 11 Dec 2021 12:33:04: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:33:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:33:05: #2 number of paired peaks: 22 WARNING @ Sat, 11 Dec 2021 12:33:05: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:33:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986169/SRX9986169.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986169/SRX9986169.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986169/SRX9986169.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986169/SRX9986169.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling