Job ID = 14171376 SRX = SRX9986167 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 24909259 spots for SRR13591521/SRR13591521.sra Written 24909259 spots for SRR13591521/SRR13591521.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171868 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:56 24909259 reads; of these: 24909259 (100.00%) were unpaired; of these: 3624062 (14.55%) aligned 0 times 16462075 (66.09%) aligned exactly 1 time 4823122 (19.36%) aligned >1 times 85.45% overall alignment rate Time searching: 00:06:56 Overall time: 00:06:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5094014 / 21285197 = 0.2393 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:39:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986167/SRX9986167.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986167/SRX9986167.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986167/SRX9986167.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986167/SRX9986167.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:39:50: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:39:50: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:39:56: 1000000 INFO @ Sat, 11 Dec 2021 11:40:01: 2000000 INFO @ Sat, 11 Dec 2021 11:40:07: 3000000 INFO @ Sat, 11 Dec 2021 11:40:12: 4000000 INFO @ Sat, 11 Dec 2021 11:40:17: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:40:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986167/SRX9986167.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986167/SRX9986167.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986167/SRX9986167.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986167/SRX9986167.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:40:20: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:40:20: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:40:23: 6000000 INFO @ Sat, 11 Dec 2021 11:40:27: 1000000 INFO @ Sat, 11 Dec 2021 11:40:30: 7000000 INFO @ Sat, 11 Dec 2021 11:40:33: 2000000 INFO @ Sat, 11 Dec 2021 11:40:36: 8000000 INFO @ Sat, 11 Dec 2021 11:40:39: 3000000 INFO @ Sat, 11 Dec 2021 11:40:42: 9000000 INFO @ Sat, 11 Dec 2021 11:40:46: 4000000 BedGraph に変換中... INFO @ Sat, 11 Dec 2021 11:40:48: 10000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:40:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986167/SRX9986167.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986167/SRX9986167.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986167/SRX9986167.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986167/SRX9986167.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:40:50: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:40:50: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:40:52: 5000000 INFO @ Sat, 11 Dec 2021 11:40:55: 11000000 INFO @ Sat, 11 Dec 2021 11:40:58: 1000000 INFO @ Sat, 11 Dec 2021 11:40:59: 6000000 INFO @ Sat, 11 Dec 2021 11:41:02: 12000000 INFO @ Sat, 11 Dec 2021 11:41:06: 7000000 INFO @ Sat, 11 Dec 2021 11:41:06: 2000000 INFO @ Sat, 11 Dec 2021 11:41:10: 13000000 INFO @ Sat, 11 Dec 2021 11:41:13: 8000000 INFO @ Sat, 11 Dec 2021 11:41:15: 3000000 INFO @ Sat, 11 Dec 2021 11:41:17: 14000000 INFO @ Sat, 11 Dec 2021 11:41:21: 9000000 INFO @ Sat, 11 Dec 2021 11:41:23: 4000000 INFO @ Sat, 11 Dec 2021 11:41:24: 15000000 INFO @ Sat, 11 Dec 2021 11:41:28: 10000000 INFO @ Sat, 11 Dec 2021 11:41:31: 5000000 INFO @ Sat, 11 Dec 2021 11:41:31: 16000000 INFO @ Sat, 11 Dec 2021 11:41:32: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 11:41:32: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 11:41:32: #1 total tags in treatment: 16191183 INFO @ Sat, 11 Dec 2021 11:41:32: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:41:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:41:33: #1 tags after filtering in treatment: 16191183 INFO @ Sat, 11 Dec 2021 11:41:33: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 11:41:33: #1 finished! INFO @ Sat, 11 Dec 2021 11:41:33: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:41:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:41:34: #2 number of paired peaks: 10 WARNING @ Sat, 11 Dec 2021 11:41:34: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 11:41:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986167/SRX9986167.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986167/SRX9986167.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986167/SRX9986167.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986167/SRX9986167.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 11:41:35: 11000000 INFO @ Sat, 11 Dec 2021 11:41:39: 6000000 INFO @ Sat, 11 Dec 2021 11:41:42: 12000000 INFO @ Sat, 11 Dec 2021 11:41:47: 7000000 INFO @ Sat, 11 Dec 2021 11:41:49: 13000000 INFO @ Sat, 11 Dec 2021 11:41:55: 8000000 INFO @ Sat, 11 Dec 2021 11:41:56: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 11:42:03: 9000000 INFO @ Sat, 11 Dec 2021 11:42:03: 15000000 INFO @ Sat, 11 Dec 2021 11:42:10: 16000000 INFO @ Sat, 11 Dec 2021 11:42:11: 10000000 INFO @ Sat, 11 Dec 2021 11:42:11: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 11:42:11: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 11:42:11: #1 total tags in treatment: 16191183 INFO @ Sat, 11 Dec 2021 11:42:11: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:42:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:42:11: #1 tags after filtering in treatment: 16191183 INFO @ Sat, 11 Dec 2021 11:42:11: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 11:42:11: #1 finished! INFO @ Sat, 11 Dec 2021 11:42:11: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:42:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:42:12: #2 number of paired peaks: 10 WARNING @ Sat, 11 Dec 2021 11:42:12: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 11:42:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986167/SRX9986167.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986167/SRX9986167.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986167/SRX9986167.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986167/SRX9986167.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 11:42:18: 11000000 INFO @ Sat, 11 Dec 2021 11:42:25: 12000000 INFO @ Sat, 11 Dec 2021 11:42:33: 13000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 11:42:40: 14000000 INFO @ Sat, 11 Dec 2021 11:42:47: 15000000 INFO @ Sat, 11 Dec 2021 11:42:55: 16000000 INFO @ Sat, 11 Dec 2021 11:42:56: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 11:42:56: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 11:42:56: #1 total tags in treatment: 16191183 INFO @ Sat, 11 Dec 2021 11:42:56: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:42:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:42:56: #1 tags after filtering in treatment: 16191183 INFO @ Sat, 11 Dec 2021 11:42:56: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 11:42:56: #1 finished! INFO @ Sat, 11 Dec 2021 11:42:56: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:42:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:42:57: #2 number of paired peaks: 10 WARNING @ Sat, 11 Dec 2021 11:42:57: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 11:42:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986167/SRX9986167.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986167/SRX9986167.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986167/SRX9986167.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986167/SRX9986167.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling