Job ID = 14171373 SRX = SRX9986164 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 15304469 spots for SRR13591567/SRR13591567.sra Written 15304469 spots for SRR13591567/SRR13591567.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171838 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:29 15304469 reads; of these: 15304469 (100.00%) were unpaired; of these: 2938497 (19.20%) aligned 0 times 10250583 (66.98%) aligned exactly 1 time 2115389 (13.82%) aligned >1 times 80.80% overall alignment rate Time searching: 00:03:29 Overall time: 00:03:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2403147 / 12365972 = 0.1943 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:33:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986164/SRX9986164.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986164/SRX9986164.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986164/SRX9986164.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986164/SRX9986164.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:33:43: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:33:43: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:33:49: 1000000 INFO @ Sat, 11 Dec 2021 11:33:55: 2000000 INFO @ Sat, 11 Dec 2021 11:34:01: 3000000 INFO @ Sat, 11 Dec 2021 11:34:07: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:34:13: 5000000 INFO @ Sat, 11 Dec 2021 11:34:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986164/SRX9986164.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986164/SRX9986164.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986164/SRX9986164.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986164/SRX9986164.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:34:13: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:34:13: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:34:19: 6000000 INFO @ Sat, 11 Dec 2021 11:34:20: 1000000 INFO @ Sat, 11 Dec 2021 11:34:26: 7000000 INFO @ Sat, 11 Dec 2021 11:34:27: 2000000 INFO @ Sat, 11 Dec 2021 11:34:32: 8000000 INFO @ Sat, 11 Dec 2021 11:34:33: 3000000 INFO @ Sat, 11 Dec 2021 11:34:39: 9000000 INFO @ Sat, 11 Dec 2021 11:34:40: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:34:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986164/SRX9986164.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986164/SRX9986164.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986164/SRX9986164.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986164/SRX9986164.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:34:43: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:34:43: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:34:45: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 11:34:45: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 11:34:45: #1 total tags in treatment: 9962825 INFO @ Sat, 11 Dec 2021 11:34:45: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:34:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:34:45: #1 tags after filtering in treatment: 9962825 INFO @ Sat, 11 Dec 2021 11:34:45: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 11:34:45: #1 finished! INFO @ Sat, 11 Dec 2021 11:34:45: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:34:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:34:46: #2 number of paired peaks: 38 WARNING @ Sat, 11 Dec 2021 11:34:46: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 11:34:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986164/SRX9986164.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986164/SRX9986164.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986164/SRX9986164.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986164/SRX9986164.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 11:34:46: 5000000 INFO @ Sat, 11 Dec 2021 11:34:50: 1000000 INFO @ Sat, 11 Dec 2021 11:34:53: 6000000 INFO @ Sat, 11 Dec 2021 11:34:57: 2000000 INFO @ Sat, 11 Dec 2021 11:35:00: 7000000 INFO @ Sat, 11 Dec 2021 11:35:04: 3000000 INFO @ Sat, 11 Dec 2021 11:35:07: 8000000 INFO @ Sat, 11 Dec 2021 11:35:10: 4000000 INFO @ Sat, 11 Dec 2021 11:35:13: 9000000 INFO @ Sat, 11 Dec 2021 11:35:17: 5000000 INFO @ Sat, 11 Dec 2021 11:35:20: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 11:35:20: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 11:35:20: #1 total tags in treatment: 9962825 INFO @ Sat, 11 Dec 2021 11:35:20: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:35:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:35:20: #1 tags after filtering in treatment: 9962825 INFO @ Sat, 11 Dec 2021 11:35:20: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 11:35:20: #1 finished! INFO @ Sat, 11 Dec 2021 11:35:20: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:35:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:35:20: #2 number of paired peaks: 38 WARNING @ Sat, 11 Dec 2021 11:35:20: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 11:35:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986164/SRX9986164.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986164/SRX9986164.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986164/SRX9986164.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986164/SRX9986164.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 11:35:23: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 11:35:29: 7000000 INFO @ Sat, 11 Dec 2021 11:35:35: 8000000 INFO @ Sat, 11 Dec 2021 11:35:40: 9000000 INFO @ Sat, 11 Dec 2021 11:35:46: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 11:35:46: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 11:35:46: #1 total tags in treatment: 9962825 INFO @ Sat, 11 Dec 2021 11:35:46: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:35:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:35:46: #1 tags after filtering in treatment: 9962825 INFO @ Sat, 11 Dec 2021 11:35:46: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 11:35:46: #1 finished! INFO @ Sat, 11 Dec 2021 11:35:46: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:35:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:35:46: #2 number of paired peaks: 38 WARNING @ Sat, 11 Dec 2021 11:35:46: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 11:35:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986164/SRX9986164.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986164/SRX9986164.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986164/SRX9986164.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986164/SRX9986164.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。