Job ID = 14171370 SRX = SRX9986161 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 20907280 spots for SRR13591516/SRR13591516.sra Written 20907280 spots for SRR13591516/SRR13591516.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171848 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:54 20907280 reads; of these: 20907280 (100.00%) were unpaired; of these: 1889568 (9.04%) aligned 0 times 14027699 (67.09%) aligned exactly 1 time 4990013 (23.87%) aligned >1 times 90.96% overall alignment rate Time searching: 00:05:54 Overall time: 00:05:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3797681 / 19017712 = 0.1997 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:37:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986161/SRX9986161.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986161/SRX9986161.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986161/SRX9986161.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986161/SRX9986161.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:37:01: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:37:01: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:37:07: 1000000 INFO @ Sat, 11 Dec 2021 11:37:13: 2000000 INFO @ Sat, 11 Dec 2021 11:37:18: 3000000 INFO @ Sat, 11 Dec 2021 11:37:24: 4000000 INFO @ Sat, 11 Dec 2021 11:37:29: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:37:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986161/SRX9986161.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986161/SRX9986161.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986161/SRX9986161.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986161/SRX9986161.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:37:31: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:37:31: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:37:34: 6000000 INFO @ Sat, 11 Dec 2021 11:37:38: 1000000 INFO @ Sat, 11 Dec 2021 11:37:40: 7000000 INFO @ Sat, 11 Dec 2021 11:37:44: 2000000 INFO @ Sat, 11 Dec 2021 11:37:45: 8000000 INFO @ Sat, 11 Dec 2021 11:37:50: 3000000 INFO @ Sat, 11 Dec 2021 11:37:51: 9000000 INFO @ Sat, 11 Dec 2021 11:37:57: 4000000 INFO @ Sat, 11 Dec 2021 11:37:57: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:38:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986161/SRX9986161.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986161/SRX9986161.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986161/SRX9986161.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986161/SRX9986161.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:38:01: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:38:01: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:38:02: 11000000 INFO @ Sat, 11 Dec 2021 11:38:03: 5000000 INFO @ Sat, 11 Dec 2021 11:38:07: 1000000 INFO @ Sat, 11 Dec 2021 11:38:08: 12000000 INFO @ Sat, 11 Dec 2021 11:38:09: 6000000 INFO @ Sat, 11 Dec 2021 11:38:13: 2000000 INFO @ Sat, 11 Dec 2021 11:38:14: 13000000 INFO @ Sat, 11 Dec 2021 11:38:16: 7000000 INFO @ Sat, 11 Dec 2021 11:38:19: 3000000 INFO @ Sat, 11 Dec 2021 11:38:19: 14000000 INFO @ Sat, 11 Dec 2021 11:38:22: 8000000 INFO @ Sat, 11 Dec 2021 11:38:25: 4000000 INFO @ Sat, 11 Dec 2021 11:38:25: 15000000 INFO @ Sat, 11 Dec 2021 11:38:26: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 11:38:26: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 11:38:26: #1 total tags in treatment: 15220031 INFO @ Sat, 11 Dec 2021 11:38:26: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:38:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:38:27: #1 tags after filtering in treatment: 15220031 INFO @ Sat, 11 Dec 2021 11:38:27: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 11:38:27: #1 finished! INFO @ Sat, 11 Dec 2021 11:38:27: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:38:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:38:28: #2 number of paired peaks: 23 WARNING @ Sat, 11 Dec 2021 11:38:28: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 11:38:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986161/SRX9986161.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis INFO @ Sat, 11 Dec 2021 11:38:28: 9000000 needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986161/SRX9986161.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986161/SRX9986161.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986161/SRX9986161.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 11:38:30: 5000000 INFO @ Sat, 11 Dec 2021 11:38:35: 10000000 INFO @ Sat, 11 Dec 2021 11:38:36: 6000000 INFO @ Sat, 11 Dec 2021 11:38:41: 11000000 INFO @ Sat, 11 Dec 2021 11:38:42: 7000000 INFO @ Sat, 11 Dec 2021 11:38:47: 12000000 INFO @ Sat, 11 Dec 2021 11:38:48: 8000000 INFO @ Sat, 11 Dec 2021 11:38:53: 9000000 INFO @ Sat, 11 Dec 2021 11:38:53: 13000000 INFO @ Sat, 11 Dec 2021 11:38:59: 10000000 INFO @ Sat, 11 Dec 2021 11:39:00: 14000000 INFO @ Sat, 11 Dec 2021 11:39:04: 11000000 INFO @ Sat, 11 Dec 2021 11:39:06: 15000000 INFO @ Sat, 11 Dec 2021 11:39:07: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 11:39:07: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 11:39:07: #1 total tags in treatment: 15220031 INFO @ Sat, 11 Dec 2021 11:39:07: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:39:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 11:39:08: #1 tags after filtering in treatment: 15220031 INFO @ Sat, 11 Dec 2021 11:39:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 11:39:08: #1 finished! INFO @ Sat, 11 Dec 2021 11:39:08: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:39:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:39:09: #2 number of paired peaks: 23 WARNING @ Sat, 11 Dec 2021 11:39:09: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 11:39:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986161/SRX9986161.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986161/SRX9986161.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986161/SRX9986161.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986161/SRX9986161.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 11:39:10: 12000000 INFO @ Sat, 11 Dec 2021 11:39:15: 13000000 INFO @ Sat, 11 Dec 2021 11:39:20: 14000000 INFO @ Sat, 11 Dec 2021 11:39:25: 15000000 INFO @ Sat, 11 Dec 2021 11:39:26: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 11:39:26: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 11:39:26: #1 total tags in treatment: 15220031 INFO @ Sat, 11 Dec 2021 11:39:26: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:39:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:39:26: #1 tags after filtering in treatment: 15220031 INFO @ Sat, 11 Dec 2021 11:39:26: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 11:39:26: #1 finished! INFO @ Sat, 11 Dec 2021 11:39:26: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:39:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:39:27: #2 number of paired peaks: 23 WARNING @ Sat, 11 Dec 2021 11:39:27: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 11:39:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986161/SRX9986161.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986161/SRX9986161.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986161/SRX9986161.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986161/SRX9986161.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。