Job ID = 14171343 SRX = SRX9986159 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 17245690 spots for SRR13591514/SRR13591514.sra Written 17245690 spots for SRR13591514/SRR13591514.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171817 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:14 17245690 reads; of these: 17245690 (100.00%) were unpaired; of these: 2306581 (13.37%) aligned 0 times 10892843 (63.16%) aligned exactly 1 time 4046266 (23.46%) aligned >1 times 86.63% overall alignment rate Time searching: 00:06:14 Overall time: 00:06:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2584044 / 14939109 = 0.1730 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:31:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986159/SRX9986159.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986159/SRX9986159.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986159/SRX9986159.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986159/SRX9986159.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:31:37: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:31:37: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:31:48: 1000000 INFO @ Sat, 11 Dec 2021 11:31:59: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:32:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986159/SRX9986159.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986159/SRX9986159.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986159/SRX9986159.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986159/SRX9986159.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:32:07: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:32:07: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:32:11: 3000000 INFO @ Sat, 11 Dec 2021 11:32:17: 1000000 INFO @ Sat, 11 Dec 2021 11:32:22: 4000000 INFO @ Sat, 11 Dec 2021 11:32:28: 2000000 INFO @ Sat, 11 Dec 2021 11:32:33: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:32:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986159/SRX9986159.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986159/SRX9986159.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986159/SRX9986159.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986159/SRX9986159.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:32:37: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:32:37: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:32:37: 3000000 INFO @ Sat, 11 Dec 2021 11:32:45: 6000000 INFO @ Sat, 11 Dec 2021 11:32:46: 4000000 INFO @ Sat, 11 Dec 2021 11:32:49: 1000000 INFO @ Sat, 11 Dec 2021 11:32:56: 5000000 INFO @ Sat, 11 Dec 2021 11:32:56: 7000000 INFO @ Sat, 11 Dec 2021 11:33:00: 2000000 INFO @ Sat, 11 Dec 2021 11:33:06: 6000000 INFO @ Sat, 11 Dec 2021 11:33:08: 8000000 INFO @ Sat, 11 Dec 2021 11:33:11: 3000000 INFO @ Sat, 11 Dec 2021 11:33:15: 7000000 INFO @ Sat, 11 Dec 2021 11:33:19: 9000000 INFO @ Sat, 11 Dec 2021 11:33:23: 4000000 INFO @ Sat, 11 Dec 2021 11:33:24: 8000000 INFO @ Sat, 11 Dec 2021 11:33:31: 10000000 INFO @ Sat, 11 Dec 2021 11:33:34: 9000000 INFO @ Sat, 11 Dec 2021 11:33:34: 5000000 INFO @ Sat, 11 Dec 2021 11:33:42: 11000000 INFO @ Sat, 11 Dec 2021 11:33:43: 10000000 INFO @ Sat, 11 Dec 2021 11:33:46: 6000000 INFO @ Sat, 11 Dec 2021 11:33:53: 11000000 INFO @ Sat, 11 Dec 2021 11:33:54: 12000000 INFO @ Sat, 11 Dec 2021 11:33:57: 7000000 INFO @ Sat, 11 Dec 2021 11:33:57: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 11:33:57: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 11:33:57: #1 total tags in treatment: 12355065 INFO @ Sat, 11 Dec 2021 11:33:57: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:33:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:33:57: #1 tags after filtering in treatment: 12355065 INFO @ Sat, 11 Dec 2021 11:33:57: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 11:33:57: #1 finished! INFO @ Sat, 11 Dec 2021 11:33:57: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:33:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:33:58: #2 number of paired peaks: 69 WARNING @ Sat, 11 Dec 2021 11:33:58: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 11:33:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986159/SRX9986159.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986159/SRX9986159.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986159/SRX9986159.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986159/SRX9986159.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 11:34:03: 12000000 INFO @ Sat, 11 Dec 2021 11:34:06: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 11:34:06: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 11:34:06: #1 total tags in treatment: 12355065 INFO @ Sat, 11 Dec 2021 11:34:06: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:34:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:34:06: #1 tags after filtering in treatment: 12355065 INFO @ Sat, 11 Dec 2021 11:34:06: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 11:34:06: #1 finished! INFO @ Sat, 11 Dec 2021 11:34:06: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:34:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:34:07: #2 number of paired peaks: 69 WARNING @ Sat, 11 Dec 2021 11:34:07: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 11:34:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986159/SRX9986159.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986159/SRX9986159.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986159/SRX9986159.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986159/SRX9986159.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 11:34:08: 8000000 INFO @ Sat, 11 Dec 2021 11:34:19: 9000000 INFO @ Sat, 11 Dec 2021 11:34:30: 10000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 11:34:41: 11000000 INFO @ Sat, 11 Dec 2021 11:34:53: 12000000 INFO @ Sat, 11 Dec 2021 11:34:56: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 11:34:56: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 11:34:56: #1 total tags in treatment: 12355065 INFO @ Sat, 11 Dec 2021 11:34:56: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:34:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:34:57: #1 tags after filtering in treatment: 12355065 INFO @ Sat, 11 Dec 2021 11:34:57: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 11:34:57: #1 finished! INFO @ Sat, 11 Dec 2021 11:34:57: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:34:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:34:57: #2 number of paired peaks: 69 WARNING @ Sat, 11 Dec 2021 11:34:57: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 11:34:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986159/SRX9986159.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986159/SRX9986159.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986159/SRX9986159.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986159/SRX9986159.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling