Job ID = 14171342 SRX = SRX9986158 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 12395455 spots for SRR13591513/SRR13591513.sra Written 12395455 spots for SRR13591513/SRR13591513.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171799 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:20 12395455 reads; of these: 12395455 (100.00%) were unpaired; of these: 1430090 (11.54%) aligned 0 times 7831229 (63.18%) aligned exactly 1 time 3134136 (25.28%) aligned >1 times 88.46% overall alignment rate Time searching: 00:03:20 Overall time: 00:03:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1719277 / 10965365 = 0.1568 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:26:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986158/SRX9986158.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986158/SRX9986158.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986158/SRX9986158.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986158/SRX9986158.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:26:08: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:26:08: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:26:13: 1000000 INFO @ Sat, 11 Dec 2021 11:26:18: 2000000 INFO @ Sat, 11 Dec 2021 11:26:24: 3000000 INFO @ Sat, 11 Dec 2021 11:26:29: 4000000 INFO @ Sat, 11 Dec 2021 11:26:34: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:26:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986158/SRX9986158.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986158/SRX9986158.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986158/SRX9986158.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986158/SRX9986158.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:26:38: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:26:38: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:26:39: 6000000 INFO @ Sat, 11 Dec 2021 11:26:43: 1000000 INFO @ Sat, 11 Dec 2021 11:26:45: 7000000 INFO @ Sat, 11 Dec 2021 11:26:49: 2000000 INFO @ Sat, 11 Dec 2021 11:26:51: 8000000 INFO @ Sat, 11 Dec 2021 11:26:55: 3000000 INFO @ Sat, 11 Dec 2021 11:26:57: 9000000 INFO @ Sat, 11 Dec 2021 11:26:58: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 11:26:58: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 11:26:58: #1 total tags in treatment: 9246088 INFO @ Sat, 11 Dec 2021 11:26:58: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:26:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:26:58: #1 tags after filtering in treatment: 9246088 INFO @ Sat, 11 Dec 2021 11:26:58: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 11:26:58: #1 finished! INFO @ Sat, 11 Dec 2021 11:26:58: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:26:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:26:59: #2 number of paired peaks: 84 WARNING @ Sat, 11 Dec 2021 11:26:59: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 11:26:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986158/SRX9986158.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986158/SRX9986158.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986158/SRX9986158.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986158/SRX9986158.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 11:27:00: 4000000 INFO @ Sat, 11 Dec 2021 11:27:06: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:27:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986158/SRX9986158.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986158/SRX9986158.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986158/SRX9986158.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986158/SRX9986158.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:27:08: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:27:08: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:27:11: 6000000 INFO @ Sat, 11 Dec 2021 11:27:13: 1000000 INFO @ Sat, 11 Dec 2021 11:27:17: 7000000 INFO @ Sat, 11 Dec 2021 11:27:19: 2000000 INFO @ Sat, 11 Dec 2021 11:27:23: 8000000 INFO @ Sat, 11 Dec 2021 11:27:25: 3000000 INFO @ Sat, 11 Dec 2021 11:27:28: 9000000 INFO @ Sat, 11 Dec 2021 11:27:30: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 11:27:30: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 11:27:30: #1 total tags in treatment: 9246088 INFO @ Sat, 11 Dec 2021 11:27:30: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:27:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:27:30: #1 tags after filtering in treatment: 9246088 INFO @ Sat, 11 Dec 2021 11:27:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 11:27:30: #1 finished! INFO @ Sat, 11 Dec 2021 11:27:30: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:27:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:27:31: #2 number of paired peaks: 84 WARNING @ Sat, 11 Dec 2021 11:27:31: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 11:27:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986158/SRX9986158.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986158/SRX9986158.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986158/SRX9986158.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986158/SRX9986158.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 11:27:31: 4000000 INFO @ Sat, 11 Dec 2021 11:27:36: 5000000 INFO @ Sat, 11 Dec 2021 11:27:41: 6000000 INFO @ Sat, 11 Dec 2021 11:27:47: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 11:27:52: 8000000 INFO @ Sat, 11 Dec 2021 11:27:57: 9000000 INFO @ Sat, 11 Dec 2021 11:27:58: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 11:27:58: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 11:27:58: #1 total tags in treatment: 9246088 INFO @ Sat, 11 Dec 2021 11:27:58: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:27:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:27:58: #1 tags after filtering in treatment: 9246088 INFO @ Sat, 11 Dec 2021 11:27:58: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 11:27:58: #1 finished! INFO @ Sat, 11 Dec 2021 11:27:58: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:27:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:27:59: #2 number of paired peaks: 84 WARNING @ Sat, 11 Dec 2021 11:27:59: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 11:27:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986158/SRX9986158.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986158/SRX9986158.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986158/SRX9986158.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986158/SRX9986158.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。