Job ID = 14171277 SRX = SRX9986130 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 27206299 spots for SRR13591549/SRR13591549.sra Written 27206299 spots for SRR13591549/SRR13591549.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171730 ("srTdm6") has been submitted Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:49 27206299 reads; of these: 27206299 (100.00%) were unpaired; of these: 2351096 (8.64%) aligned 0 times 20959691 (77.04%) aligned exactly 1 time 3895512 (14.32%) aligned >1 times 91.36% overall alignment rate Time searching: 00:06:50 Overall time: 00:06:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 9171457 / 24855203 = 0.3690 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:05:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986130/SRX9986130.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986130/SRX9986130.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986130/SRX9986130.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986130/SRX9986130.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:05:34: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:05:34: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:05:42: 1000000 INFO @ Sat, 11 Dec 2021 11:05:51: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:06:00: 3000000 INFO @ Sat, 11 Dec 2021 11:06:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986130/SRX9986130.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986130/SRX9986130.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986130/SRX9986130.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986130/SRX9986130.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:06:02: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:06:02: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:06:08: 4000000 INFO @ Sat, 11 Dec 2021 11:06:12: 1000000 INFO @ Sat, 11 Dec 2021 11:06:17: 5000000 INFO @ Sat, 11 Dec 2021 11:06:22: 2000000 INFO @ Sat, 11 Dec 2021 11:06:25: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:06:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986130/SRX9986130.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986130/SRX9986130.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986130/SRX9986130.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986130/SRX9986130.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:06:32: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:06:32: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:06:33: 3000000 INFO @ Sat, 11 Dec 2021 11:06:34: 7000000 INFO @ Sat, 11 Dec 2021 11:06:40: 1000000 INFO @ Sat, 11 Dec 2021 11:06:43: 8000000 INFO @ Sat, 11 Dec 2021 11:06:43: 4000000 INFO @ Sat, 11 Dec 2021 11:06:49: 2000000 INFO @ Sat, 11 Dec 2021 11:06:51: 9000000 INFO @ Sat, 11 Dec 2021 11:06:54: 5000000 INFO @ Sat, 11 Dec 2021 11:06:57: 3000000 INFO @ Sat, 11 Dec 2021 11:06:59: 10000000 INFO @ Sat, 11 Dec 2021 11:07:04: 6000000 INFO @ Sat, 11 Dec 2021 11:07:05: 4000000 INFO @ Sat, 11 Dec 2021 11:07:08: 11000000 INFO @ Sat, 11 Dec 2021 11:07:13: 5000000 INFO @ Sat, 11 Dec 2021 11:07:14: 7000000 INFO @ Sat, 11 Dec 2021 11:07:17: 12000000 INFO @ Sat, 11 Dec 2021 11:07:21: 6000000 INFO @ Sat, 11 Dec 2021 11:07:25: 8000000 INFO @ Sat, 11 Dec 2021 11:07:25: 13000000 INFO @ Sat, 11 Dec 2021 11:07:30: 7000000 INFO @ Sat, 11 Dec 2021 11:07:33: 14000000 INFO @ Sat, 11 Dec 2021 11:07:35: 9000000 INFO @ Sat, 11 Dec 2021 11:07:38: 8000000 INFO @ Sat, 11 Dec 2021 11:07:42: 15000000 INFO @ Sat, 11 Dec 2021 11:07:45: 10000000 INFO @ Sat, 11 Dec 2021 11:07:46: 9000000 INFO @ Sat, 11 Dec 2021 11:07:47: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 11:07:47: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 11:07:47: #1 total tags in treatment: 15683746 INFO @ Sat, 11 Dec 2021 11:07:47: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:07:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:07:47: #1 tags after filtering in treatment: 15683746 INFO @ Sat, 11 Dec 2021 11:07:47: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 11:07:47: #1 finished! INFO @ Sat, 11 Dec 2021 11:07:47: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:07:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:07:49: #2 number of paired peaks: 83 WARNING @ Sat, 11 Dec 2021 11:07:49: Too few paired peaks (83) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 11:07:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986130/SRX9986130.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986130/SRX9986130.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986130/SRX9986130.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986130/SRX9986130.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 11:07:54: 10000000 INFO @ Sat, 11 Dec 2021 11:07:55: 11000000 INFO @ Sat, 11 Dec 2021 11:08:01: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 11:08:06: 12000000 INFO @ Sat, 11 Dec 2021 11:08:09: 12000000 INFO @ Sat, 11 Dec 2021 11:08:16: 13000000 INFO @ Sat, 11 Dec 2021 11:08:17: 13000000 INFO @ Sat, 11 Dec 2021 11:08:25: 14000000 INFO @ Sat, 11 Dec 2021 11:08:26: 14000000 INFO @ Sat, 11 Dec 2021 11:08:33: 15000000 INFO @ Sat, 11 Dec 2021 11:08:36: 15000000 INFO @ Sat, 11 Dec 2021 11:08:38: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 11:08:38: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 11:08:38: #1 total tags in treatment: 15683746 INFO @ Sat, 11 Dec 2021 11:08:38: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:08:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:08:39: #1 tags after filtering in treatment: 15683746 INFO @ Sat, 11 Dec 2021 11:08:39: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 11:08:39: #1 finished! INFO @ Sat, 11 Dec 2021 11:08:39: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:08:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:08:40: #2 number of paired peaks: 83 WARNING @ Sat, 11 Dec 2021 11:08:40: Too few paired peaks (83) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 11:08:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986130/SRX9986130.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986130/SRX9986130.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986130/SRX9986130.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986130/SRX9986130.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 11:08:43: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 11:08:43: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 11:08:43: #1 total tags in treatment: 15683746 INFO @ Sat, 11 Dec 2021 11:08:43: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:08:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:08:43: #1 tags after filtering in treatment: 15683746 INFO @ Sat, 11 Dec 2021 11:08:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 11:08:43: #1 finished! INFO @ Sat, 11 Dec 2021 11:08:43: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:08:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:08:44: #2 number of paired peaks: 83 WARNING @ Sat, 11 Dec 2021 11:08:44: Too few paired peaks (83) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 11:08:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986130/SRX9986130.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis BigWig に変換しました。 needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986130/SRX9986130.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986130/SRX9986130.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986130/SRX9986130.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling