Job ID = 14171253 SRX = SRX9986127 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 27241297 spots for SRR13591546/SRR13591546.sra Written 27241297 spots for SRR13591546/SRR13591546.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171715 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:56 27241297 reads; of these: 27241297 (100.00%) were unpaired; of these: 2174221 (7.98%) aligned 0 times 21264189 (78.06%) aligned exactly 1 time 3802887 (13.96%) aligned >1 times 92.02% overall alignment rate Time searching: 00:05:56 Overall time: 00:05:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 9416867 / 25067076 = 0.3757 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:55:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986127/SRX9986127.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986127/SRX9986127.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986127/SRX9986127.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986127/SRX9986127.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:55:01: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:55:01: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:55:06: 1000000 INFO @ Sat, 11 Dec 2021 10:55:12: 2000000 INFO @ Sat, 11 Dec 2021 10:55:17: 3000000 INFO @ Sat, 11 Dec 2021 10:55:23: 4000000 INFO @ Sat, 11 Dec 2021 10:55:28: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:55:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986127/SRX9986127.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986127/SRX9986127.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986127/SRX9986127.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986127/SRX9986127.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:55:31: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:55:31: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:55:34: 6000000 INFO @ Sat, 11 Dec 2021 10:55:37: 1000000 INFO @ Sat, 11 Dec 2021 10:55:39: 7000000 INFO @ Sat, 11 Dec 2021 10:55:43: 2000000 INFO @ Sat, 11 Dec 2021 10:55:45: 8000000 INFO @ Sat, 11 Dec 2021 10:55:48: 3000000 INFO @ Sat, 11 Dec 2021 10:55:51: 9000000 INFO @ Sat, 11 Dec 2021 10:55:54: 4000000 INFO @ Sat, 11 Dec 2021 10:55:57: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:56:00: 5000000 INFO @ Sat, 11 Dec 2021 10:56:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986127/SRX9986127.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986127/SRX9986127.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986127/SRX9986127.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986127/SRX9986127.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:56:01: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:56:01: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:56:03: 11000000 INFO @ Sat, 11 Dec 2021 10:56:06: 6000000 INFO @ Sat, 11 Dec 2021 10:56:07: 1000000 INFO @ Sat, 11 Dec 2021 10:56:09: 12000000 INFO @ Sat, 11 Dec 2021 10:56:12: 7000000 INFO @ Sat, 11 Dec 2021 10:56:13: 2000000 INFO @ Sat, 11 Dec 2021 10:56:15: 13000000 INFO @ Sat, 11 Dec 2021 10:56:18: 8000000 INFO @ Sat, 11 Dec 2021 10:56:19: 3000000 INFO @ Sat, 11 Dec 2021 10:56:20: 14000000 INFO @ Sat, 11 Dec 2021 10:56:24: 9000000 INFO @ Sat, 11 Dec 2021 10:56:24: 4000000 INFO @ Sat, 11 Dec 2021 10:56:26: 15000000 INFO @ Sat, 11 Dec 2021 10:56:30: 10000000 INFO @ Sat, 11 Dec 2021 10:56:30: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 10:56:30: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 10:56:30: #1 total tags in treatment: 15650209 INFO @ Sat, 11 Dec 2021 10:56:30: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:56:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:56:30: 5000000 INFO @ Sat, 11 Dec 2021 10:56:30: #1 tags after filtering in treatment: 15650209 INFO @ Sat, 11 Dec 2021 10:56:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:56:30: #1 finished! INFO @ Sat, 11 Dec 2021 10:56:30: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:56:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:56:31: #2 number of paired peaks: 98 WARNING @ Sat, 11 Dec 2021 10:56:31: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:56:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986127/SRX9986127.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986127/SRX9986127.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986127/SRX9986127.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986127/SRX9986127.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 10:56:35: 11000000 INFO @ Sat, 11 Dec 2021 10:56:36: 6000000 INFO @ Sat, 11 Dec 2021 10:56:41: 12000000 INFO @ Sat, 11 Dec 2021 10:56:42: 7000000 INFO @ Sat, 11 Dec 2021 10:56:47: 13000000 INFO @ Sat, 11 Dec 2021 10:56:48: 8000000 INFO @ Sat, 11 Dec 2021 10:56:53: 14000000 INFO @ Sat, 11 Dec 2021 10:56:54: 9000000 INFO @ Sat, 11 Dec 2021 10:56:59: 15000000 INFO @ Sat, 11 Dec 2021 10:57:00: 10000000 INFO @ Sat, 11 Dec 2021 10:57:03: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 10:57:03: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 10:57:03: #1 total tags in treatment: 15650209 INFO @ Sat, 11 Dec 2021 10:57:03: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:57:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:57:03: #1 tags after filtering in treatment: 15650209 INFO @ Sat, 11 Dec 2021 10:57:03: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:57:03: #1 finished! INFO @ Sat, 11 Dec 2021 10:57:03: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:57:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:57:04: #2 number of paired peaks: 98 WARNING @ Sat, 11 Dec 2021 10:57:04: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:57:04: Process for pairing-model is terminated! BedGraph に変換しました。 BigWig に変換中... cut: /home/okishinya/chipatlas/results/dm3/SRX9986127/SRX9986127.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986127/SRX9986127.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986127/SRX9986127.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986127/SRX9986127.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 10:57:06: 11000000 INFO @ Sat, 11 Dec 2021 10:57:11: 12000000 INFO @ Sat, 11 Dec 2021 10:57:17: 13000000 INFO @ Sat, 11 Dec 2021 10:57:23: 14000000 INFO @ Sat, 11 Dec 2021 10:57:28: 15000000 INFO @ Sat, 11 Dec 2021 10:57:32: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 10:57:32: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 10:57:32: #1 total tags in treatment: 15650209 INFO @ Sat, 11 Dec 2021 10:57:32: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:57:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:57:32: #1 tags after filtering in treatment: 15650209 INFO @ Sat, 11 Dec 2021 10:57:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:57:32: #1 finished! INFO @ Sat, 11 Dec 2021 10:57:32: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:57:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:57:33: #2 number of paired peaks: 98 WARNING @ Sat, 11 Dec 2021 10:57:33: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:57:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986127/SRX9986127.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986127/SRX9986127.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986127/SRX9986127.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986127/SRX9986127.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。