Job ID = 14171199 SRX = SRX9986119 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 16487570 spots for SRR13591538/SRR13591538.sra Written 16487570 spots for SRR13591538/SRR13591538.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171674 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:58 16487570 reads; of these: 16487570 (100.00%) were unpaired; of these: 1922852 (11.66%) aligned 0 times 11892461 (72.13%) aligned exactly 1 time 2672257 (16.21%) aligned >1 times 88.34% overall alignment rate Time searching: 00:03:58 Overall time: 00:03:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2942834 / 14564718 = 0.2021 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:39:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986119/SRX9986119.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986119/SRX9986119.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986119/SRX9986119.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986119/SRX9986119.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:39:11: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:39:11: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:39:17: 1000000 INFO @ Sat, 11 Dec 2021 10:39:23: 2000000 INFO @ Sat, 11 Dec 2021 10:39:28: 3000000 INFO @ Sat, 11 Dec 2021 10:39:33: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:39:39: 5000000 INFO @ Sat, 11 Dec 2021 10:39:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986119/SRX9986119.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986119/SRX9986119.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986119/SRX9986119.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986119/SRX9986119.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:39:40: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:39:40: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:39:45: 6000000 INFO @ Sat, 11 Dec 2021 10:39:46: 1000000 INFO @ Sat, 11 Dec 2021 10:39:51: 7000000 INFO @ Sat, 11 Dec 2021 10:39:52: 2000000 INFO @ Sat, 11 Dec 2021 10:39:57: 8000000 INFO @ Sat, 11 Dec 2021 10:39:57: 3000000 INFO @ Sat, 11 Dec 2021 10:40:03: 4000000 INFO @ Sat, 11 Dec 2021 10:40:03: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:40:09: 5000000 INFO @ Sat, 11 Dec 2021 10:40:09: 10000000 INFO @ Sat, 11 Dec 2021 10:40:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986119/SRX9986119.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986119/SRX9986119.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986119/SRX9986119.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986119/SRX9986119.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:40:11: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:40:11: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:40:14: 6000000 INFO @ Sat, 11 Dec 2021 10:40:15: 11000000 INFO @ Sat, 11 Dec 2021 10:40:16: 1000000 INFO @ Sat, 11 Dec 2021 10:40:19: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 10:40:19: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 10:40:19: #1 total tags in treatment: 11621884 INFO @ Sat, 11 Dec 2021 10:40:19: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:40:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:40:19: #1 tags after filtering in treatment: 11621884 INFO @ Sat, 11 Dec 2021 10:40:19: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:40:19: #1 finished! INFO @ Sat, 11 Dec 2021 10:40:19: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:40:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:40:20: #2 number of paired peaks: 35 WARNING @ Sat, 11 Dec 2021 10:40:20: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:40:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986119/SRX9986119.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986119/SRX9986119.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986119/SRX9986119.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986119/SRX9986119.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 10:40:20: 7000000 INFO @ Sat, 11 Dec 2021 10:40:22: 2000000 INFO @ Sat, 11 Dec 2021 10:40:26: 8000000 INFO @ Sat, 11 Dec 2021 10:40:27: 3000000 INFO @ Sat, 11 Dec 2021 10:40:31: 9000000 INFO @ Sat, 11 Dec 2021 10:40:33: 4000000 INFO @ Sat, 11 Dec 2021 10:40:37: 10000000 INFO @ Sat, 11 Dec 2021 10:40:38: 5000000 INFO @ Sat, 11 Dec 2021 10:40:42: 11000000 INFO @ Sat, 11 Dec 2021 10:40:44: 6000000 INFO @ Sat, 11 Dec 2021 10:40:46: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 10:40:46: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 10:40:46: #1 total tags in treatment: 11621884 INFO @ Sat, 11 Dec 2021 10:40:46: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:40:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:40:46: #1 tags after filtering in treatment: 11621884 INFO @ Sat, 11 Dec 2021 10:40:46: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:40:46: #1 finished! INFO @ Sat, 11 Dec 2021 10:40:46: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:40:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:40:47: #2 number of paired peaks: 35 WARNING @ Sat, 11 Dec 2021 10:40:47: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:40:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986119/SRX9986119.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986119/SRX9986119.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986119/SRX9986119.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986119/SRX9986119.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 10:40:49: 7000000 INFO @ Sat, 11 Dec 2021 10:40:54: 8000000 INFO @ Sat, 11 Dec 2021 10:40:59: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 10:41:04: 10000000 INFO @ Sat, 11 Dec 2021 10:41:09: 11000000 INFO @ Sat, 11 Dec 2021 10:41:13: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 10:41:13: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 10:41:13: #1 total tags in treatment: 11621884 INFO @ Sat, 11 Dec 2021 10:41:13: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:41:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:41:13: #1 tags after filtering in treatment: 11621884 INFO @ Sat, 11 Dec 2021 10:41:13: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:41:13: #1 finished! INFO @ Sat, 11 Dec 2021 10:41:13: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:41:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:41:13: #2 number of paired peaks: 35 WARNING @ Sat, 11 Dec 2021 10:41:13: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:41:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986119/SRX9986119.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986119/SRX9986119.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986119/SRX9986119.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986119/SRX9986119.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。