Job ID = 14171191 SRX = SRX9986115 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 19896350 spots for SRR13591534/SRR13591534.sra Written 19896350 spots for SRR13591534/SRR13591534.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171676 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:54 19896350 reads; of these: 19896350 (100.00%) were unpaired; of these: 2501887 (12.57%) aligned 0 times 14329328 (72.02%) aligned exactly 1 time 3065135 (15.41%) aligned >1 times 87.43% overall alignment rate Time searching: 00:05:54 Overall time: 00:05:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3904157 / 17394463 = 0.2244 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:41:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986115/SRX9986115.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986115/SRX9986115.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986115/SRX9986115.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986115/SRX9986115.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:41:58: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:41:58: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:42:07: 1000000 INFO @ Sat, 11 Dec 2021 10:42:15: 2000000 INFO @ Sat, 11 Dec 2021 10:42:24: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:42:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986115/SRX9986115.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986115/SRX9986115.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986115/SRX9986115.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986115/SRX9986115.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:42:28: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:42:28: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:42:33: 4000000 INFO @ Sat, 11 Dec 2021 10:42:36: 1000000 INFO @ Sat, 11 Dec 2021 10:42:42: 5000000 INFO @ Sat, 11 Dec 2021 10:42:43: 2000000 INFO @ Sat, 11 Dec 2021 10:42:50: 3000000 INFO @ Sat, 11 Dec 2021 10:42:50: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:42:57: 4000000 INFO @ Sat, 11 Dec 2021 10:42:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986115/SRX9986115.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986115/SRX9986115.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986115/SRX9986115.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986115/SRX9986115.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:42:58: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:42:58: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:42:59: 7000000 INFO @ Sat, 11 Dec 2021 10:43:04: 5000000 INFO @ Sat, 11 Dec 2021 10:43:06: 1000000 INFO @ Sat, 11 Dec 2021 10:43:08: 8000000 INFO @ Sat, 11 Dec 2021 10:43:11: 6000000 INFO @ Sat, 11 Dec 2021 10:43:13: 2000000 INFO @ Sat, 11 Dec 2021 10:43:17: 9000000 INFO @ Sat, 11 Dec 2021 10:43:18: 7000000 INFO @ Sat, 11 Dec 2021 10:43:20: 3000000 INFO @ Sat, 11 Dec 2021 10:43:25: 8000000 INFO @ Sat, 11 Dec 2021 10:43:26: 10000000 INFO @ Sat, 11 Dec 2021 10:43:27: 4000000 INFO @ Sat, 11 Dec 2021 10:43:32: 9000000 INFO @ Sat, 11 Dec 2021 10:43:34: 5000000 INFO @ Sat, 11 Dec 2021 10:43:35: 11000000 INFO @ Sat, 11 Dec 2021 10:43:39: 10000000 INFO @ Sat, 11 Dec 2021 10:43:41: 6000000 INFO @ Sat, 11 Dec 2021 10:43:43: 12000000 INFO @ Sat, 11 Dec 2021 10:43:47: 11000000 INFO @ Sat, 11 Dec 2021 10:43:48: 7000000 INFO @ Sat, 11 Dec 2021 10:43:52: 13000000 INFO @ Sat, 11 Dec 2021 10:43:54: 12000000 INFO @ Sat, 11 Dec 2021 10:43:56: 8000000 INFO @ Sat, 11 Dec 2021 10:43:56: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 10:43:56: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 10:43:56: #1 total tags in treatment: 13490306 INFO @ Sat, 11 Dec 2021 10:43:56: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:43:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:43:57: #1 tags after filtering in treatment: 13490306 INFO @ Sat, 11 Dec 2021 10:43:57: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:43:57: #1 finished! INFO @ Sat, 11 Dec 2021 10:43:57: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:43:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:43:58: #2 number of paired peaks: 31 WARNING @ Sat, 11 Dec 2021 10:43:58: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:43:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986115/SRX9986115.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986115/SRX9986115.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986115/SRX9986115.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986115/SRX9986115.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 10:44:01: 13000000 INFO @ Sat, 11 Dec 2021 10:44:03: 9000000 INFO @ Sat, 11 Dec 2021 10:44:04: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 10:44:04: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 10:44:04: #1 total tags in treatment: 13490306 INFO @ Sat, 11 Dec 2021 10:44:04: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:44:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:44:05: #1 tags after filtering in treatment: 13490306 INFO @ Sat, 11 Dec 2021 10:44:05: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:44:05: #1 finished! INFO @ Sat, 11 Dec 2021 10:44:05: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:44:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:44:06: #2 number of paired peaks: 31 WARNING @ Sat, 11 Dec 2021 10:44:06: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:44:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986115/SRX9986115.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986115/SRX9986115.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986115/SRX9986115.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986115/SRX9986115.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 10:44:10: 10000000 INFO @ Sat, 11 Dec 2021 10:44:17: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 10:44:23: 12000000 INFO @ Sat, 11 Dec 2021 10:44:30: 13000000 INFO @ Sat, 11 Dec 2021 10:44:34: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 10:44:34: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 10:44:34: #1 total tags in treatment: 13490306 INFO @ Sat, 11 Dec 2021 10:44:34: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:44:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:44:34: #1 tags after filtering in treatment: 13490306 INFO @ Sat, 11 Dec 2021 10:44:34: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:44:34: #1 finished! INFO @ Sat, 11 Dec 2021 10:44:34: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:44:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:44:35: #2 number of paired peaks: 31 WARNING @ Sat, 11 Dec 2021 10:44:35: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:44:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986115/SRX9986115.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986115/SRX9986115.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986115/SRX9986115.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986115/SRX9986115.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。