Job ID = 14171167 SRX = SRX9986109 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 51960776 spots for SRR13591528/SRR13591528.sra Written 51960776 spots for SRR13591528/SRR13591528.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171686 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:20:01 51960776 reads; of these: 51960776 (100.00%) were unpaired; of these: 4553722 (8.76%) aligned 0 times 33215824 (63.92%) aligned exactly 1 time 14191230 (27.31%) aligned >1 times 91.24% overall alignment rate Time searching: 00:20:01 Overall time: 00:20:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 15403459 / 47407054 = 0.3249 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:52:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986109/SRX9986109.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986109/SRX9986109.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986109/SRX9986109.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986109/SRX9986109.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:52:42: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:52:42: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:52:51: 1000000 INFO @ Sat, 11 Dec 2021 10:53:00: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:53:09: 3000000 INFO @ Sat, 11 Dec 2021 10:53:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986109/SRX9986109.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986109/SRX9986109.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986109/SRX9986109.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986109/SRX9986109.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:53:12: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:53:12: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:53:19: 4000000 INFO @ Sat, 11 Dec 2021 10:53:23: 1000000 INFO @ Sat, 11 Dec 2021 10:53:29: 5000000 INFO @ Sat, 11 Dec 2021 10:53:34: 2000000 INFO @ Sat, 11 Dec 2021 10:53:38: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:53:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986109/SRX9986109.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986109/SRX9986109.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986109/SRX9986109.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986109/SRX9986109.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:53:42: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:53:42: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:53:46: 3000000 INFO @ Sat, 11 Dec 2021 10:53:48: 7000000 INFO @ Sat, 11 Dec 2021 10:53:54: 1000000 INFO @ Sat, 11 Dec 2021 10:53:57: 4000000 INFO @ Sat, 11 Dec 2021 10:53:58: 8000000 INFO @ Sat, 11 Dec 2021 10:54:05: 2000000 INFO @ Sat, 11 Dec 2021 10:54:08: 9000000 INFO @ Sat, 11 Dec 2021 10:54:08: 5000000 INFO @ Sat, 11 Dec 2021 10:54:17: 3000000 INFO @ Sat, 11 Dec 2021 10:54:19: 10000000 INFO @ Sat, 11 Dec 2021 10:54:20: 6000000 INFO @ Sat, 11 Dec 2021 10:54:29: 11000000 INFO @ Sat, 11 Dec 2021 10:54:29: 4000000 INFO @ Sat, 11 Dec 2021 10:54:32: 7000000 INFO @ Sat, 11 Dec 2021 10:54:39: 12000000 INFO @ Sat, 11 Dec 2021 10:54:41: 5000000 INFO @ Sat, 11 Dec 2021 10:54:43: 8000000 INFO @ Sat, 11 Dec 2021 10:54:49: 13000000 INFO @ Sat, 11 Dec 2021 10:54:53: 6000000 INFO @ Sat, 11 Dec 2021 10:54:54: 9000000 INFO @ Sat, 11 Dec 2021 10:54:59: 14000000 INFO @ Sat, 11 Dec 2021 10:55:05: 10000000 INFO @ Sat, 11 Dec 2021 10:55:05: 7000000 INFO @ Sat, 11 Dec 2021 10:55:09: 15000000 INFO @ Sat, 11 Dec 2021 10:55:16: 11000000 INFO @ Sat, 11 Dec 2021 10:55:18: 8000000 INFO @ Sat, 11 Dec 2021 10:55:19: 16000000 INFO @ Sat, 11 Dec 2021 10:55:28: 12000000 INFO @ Sat, 11 Dec 2021 10:55:29: 17000000 INFO @ Sat, 11 Dec 2021 10:55:30: 9000000 INFO @ Sat, 11 Dec 2021 10:55:39: 18000000 INFO @ Sat, 11 Dec 2021 10:55:39: 13000000 INFO @ Sat, 11 Dec 2021 10:55:41: 10000000 INFO @ Sat, 11 Dec 2021 10:55:49: 19000000 INFO @ Sat, 11 Dec 2021 10:55:51: 14000000 INFO @ Sat, 11 Dec 2021 10:55:53: 11000000 INFO @ Sat, 11 Dec 2021 10:55:59: 20000000 INFO @ Sat, 11 Dec 2021 10:56:02: 15000000 INFO @ Sat, 11 Dec 2021 10:56:05: 12000000 INFO @ Sat, 11 Dec 2021 10:56:09: 21000000 INFO @ Sat, 11 Dec 2021 10:56:13: 16000000 INFO @ Sat, 11 Dec 2021 10:56:18: 13000000 INFO @ Sat, 11 Dec 2021 10:56:19: 22000000 INFO @ Sat, 11 Dec 2021 10:56:25: 17000000 INFO @ Sat, 11 Dec 2021 10:56:30: 23000000 INFO @ Sat, 11 Dec 2021 10:56:30: 14000000 INFO @ Sat, 11 Dec 2021 10:56:36: 18000000 INFO @ Sat, 11 Dec 2021 10:56:40: 24000000 INFO @ Sat, 11 Dec 2021 10:56:42: 15000000 INFO @ Sat, 11 Dec 2021 10:56:48: 19000000 INFO @ Sat, 11 Dec 2021 10:56:50: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 10:56:55: 16000000 INFO @ Sat, 11 Dec 2021 10:56:59: 20000000 INFO @ Sat, 11 Dec 2021 10:57:00: 26000000 INFO @ Sat, 11 Dec 2021 10:57:06: 17000000 INFO @ Sat, 11 Dec 2021 10:57:10: 27000000 INFO @ Sat, 11 Dec 2021 10:57:10: 21000000 INFO @ Sat, 11 Dec 2021 10:57:19: 18000000 INFO @ Sat, 11 Dec 2021 10:57:20: 28000000 INFO @ Sat, 11 Dec 2021 10:57:22: 22000000 INFO @ Sat, 11 Dec 2021 10:57:30: 29000000 INFO @ Sat, 11 Dec 2021 10:57:31: 19000000 INFO @ Sat, 11 Dec 2021 10:57:33: 23000000 INFO @ Sat, 11 Dec 2021 10:57:40: 30000000 INFO @ Sat, 11 Dec 2021 10:57:43: 20000000 INFO @ Sat, 11 Dec 2021 10:57:45: 24000000 INFO @ Sat, 11 Dec 2021 10:57:50: 31000000 INFO @ Sat, 11 Dec 2021 10:57:55: 21000000 INFO @ Sat, 11 Dec 2021 10:57:56: 25000000 INFO @ Sat, 11 Dec 2021 10:58:00: 32000000 INFO @ Sat, 11 Dec 2021 10:58:01: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 10:58:01: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 10:58:01: #1 total tags in treatment: 32003595 INFO @ Sat, 11 Dec 2021 10:58:01: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:58:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:58:01: #1 tags after filtering in treatment: 32003595 INFO @ Sat, 11 Dec 2021 10:58:01: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:58:01: #1 finished! INFO @ Sat, 11 Dec 2021 10:58:01: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:58:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:58:04: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 10:58:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:58:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986109/SRX9986109.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986109/SRX9986109.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986109/SRX9986109.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986109/SRX9986109.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 10:58:06: 22000000 INFO @ Sat, 11 Dec 2021 10:58:08: 26000000 INFO @ Sat, 11 Dec 2021 10:58:17: 23000000 INFO @ Sat, 11 Dec 2021 10:58:19: 27000000 INFO @ Sat, 11 Dec 2021 10:58:30: 24000000 INFO @ Sat, 11 Dec 2021 10:58:30: 28000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 10:58:42: 29000000 INFO @ Sat, 11 Dec 2021 10:58:42: 25000000 INFO @ Sat, 11 Dec 2021 10:58:53: 30000000 INFO @ Sat, 11 Dec 2021 10:58:54: 26000000 INFO @ Sat, 11 Dec 2021 10:59:04: 31000000 INFO @ Sat, 11 Dec 2021 10:59:06: 27000000 INFO @ Sat, 11 Dec 2021 10:59:15: 32000000 INFO @ Sat, 11 Dec 2021 10:59:16: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 10:59:16: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 10:59:16: #1 total tags in treatment: 32003595 INFO @ Sat, 11 Dec 2021 10:59:16: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:59:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:59:16: #1 tags after filtering in treatment: 32003595 INFO @ Sat, 11 Dec 2021 10:59:16: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:59:16: #1 finished! INFO @ Sat, 11 Dec 2021 10:59:16: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:59:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:59:17: 28000000 INFO @ Sat, 11 Dec 2021 10:59:19: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 10:59:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:59:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986109/SRX9986109.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986109/SRX9986109.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986109/SRX9986109.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986109/SRX9986109.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 10:59:29: 29000000 INFO @ Sat, 11 Dec 2021 10:59:40: 30000000 INFO @ Sat, 11 Dec 2021 10:59:52: 31000000 INFO @ Sat, 11 Dec 2021 11:00:03: 32000000 INFO @ Sat, 11 Dec 2021 11:00:03: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 11:00:03: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 11:00:03: #1 total tags in treatment: 32003595 INFO @ Sat, 11 Dec 2021 11:00:03: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:00:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:00:04: #1 tags after filtering in treatment: 32003595 INFO @ Sat, 11 Dec 2021 11:00:04: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 11:00:04: #1 finished! INFO @ Sat, 11 Dec 2021 11:00:04: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:00:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:00:07: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 11:00:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 11:00:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986109/SRX9986109.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986109/SRX9986109.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986109/SRX9986109.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986109/SRX9986109.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling