Job ID = 14171153 SRX = SRX9986102 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 32797421 spots for SRR13591496/SRR13591496.sra Written 32797421 spots for SRR13591496/SRR13591496.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171685 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:27:40 32797421 reads; of these: 32797421 (100.00%) were unpaired; of these: 1624742 (4.95%) aligned 0 times 21769430 (66.38%) aligned exactly 1 time 9403249 (28.67%) aligned >1 times 95.05% overall alignment rate Time searching: 00:27:40 Overall time: 00:27:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 10121877 / 31172679 = 0.3247 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:50:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986102/SRX9986102.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986102/SRX9986102.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986102/SRX9986102.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986102/SRX9986102.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:50:54: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:50:54: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:51:04: 1000000 INFO @ Sat, 11 Dec 2021 10:51:14: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:51:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986102/SRX9986102.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986102/SRX9986102.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986102/SRX9986102.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986102/SRX9986102.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:51:23: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:51:23: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:51:24: 3000000 INFO @ Sat, 11 Dec 2021 10:51:34: 1000000 INFO @ Sat, 11 Dec 2021 10:51:35: 4000000 INFO @ Sat, 11 Dec 2021 10:51:44: 2000000 INFO @ Sat, 11 Dec 2021 10:51:46: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:51:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986102/SRX9986102.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986102/SRX9986102.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986102/SRX9986102.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986102/SRX9986102.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:51:53: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:51:53: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:51:55: 3000000 INFO @ Sat, 11 Dec 2021 10:51:56: 6000000 INFO @ Sat, 11 Dec 2021 10:52:04: 1000000 INFO @ Sat, 11 Dec 2021 10:52:05: 4000000 INFO @ Sat, 11 Dec 2021 10:52:07: 7000000 INFO @ Sat, 11 Dec 2021 10:52:15: 2000000 INFO @ Sat, 11 Dec 2021 10:52:16: 5000000 INFO @ Sat, 11 Dec 2021 10:52:17: 8000000 INFO @ Sat, 11 Dec 2021 10:52:26: 3000000 INFO @ Sat, 11 Dec 2021 10:52:27: 6000000 INFO @ Sat, 11 Dec 2021 10:52:27: 9000000 INFO @ Sat, 11 Dec 2021 10:52:37: 4000000 INFO @ Sat, 11 Dec 2021 10:52:37: 7000000 INFO @ Sat, 11 Dec 2021 10:52:37: 10000000 INFO @ Sat, 11 Dec 2021 10:52:47: 5000000 INFO @ Sat, 11 Dec 2021 10:52:48: 8000000 INFO @ Sat, 11 Dec 2021 10:52:48: 11000000 INFO @ Sat, 11 Dec 2021 10:52:58: 6000000 INFO @ Sat, 11 Dec 2021 10:52:58: 9000000 INFO @ Sat, 11 Dec 2021 10:52:59: 12000000 INFO @ Sat, 11 Dec 2021 10:53:09: 7000000 INFO @ Sat, 11 Dec 2021 10:53:09: 10000000 INFO @ Sat, 11 Dec 2021 10:53:10: 13000000 INFO @ Sat, 11 Dec 2021 10:53:19: 8000000 INFO @ Sat, 11 Dec 2021 10:53:21: 11000000 INFO @ Sat, 11 Dec 2021 10:53:21: 14000000 INFO @ Sat, 11 Dec 2021 10:53:29: 9000000 INFO @ Sat, 11 Dec 2021 10:53:31: 12000000 INFO @ Sat, 11 Dec 2021 10:53:32: 15000000 INFO @ Sat, 11 Dec 2021 10:53:40: 10000000 INFO @ Sat, 11 Dec 2021 10:53:42: 13000000 INFO @ Sat, 11 Dec 2021 10:53:42: 16000000 INFO @ Sat, 11 Dec 2021 10:53:50: 11000000 INFO @ Sat, 11 Dec 2021 10:53:53: 14000000 INFO @ Sat, 11 Dec 2021 10:53:54: 17000000 INFO @ Sat, 11 Dec 2021 10:54:00: 12000000 INFO @ Sat, 11 Dec 2021 10:54:05: 15000000 INFO @ Sat, 11 Dec 2021 10:54:05: 18000000 INFO @ Sat, 11 Dec 2021 10:54:11: 13000000 INFO @ Sat, 11 Dec 2021 10:54:15: 16000000 INFO @ Sat, 11 Dec 2021 10:54:16: 19000000 INFO @ Sat, 11 Dec 2021 10:54:21: 14000000 INFO @ Sat, 11 Dec 2021 10:54:26: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 10:54:26: 20000000 INFO @ Sat, 11 Dec 2021 10:54:32: 15000000 INFO @ Sat, 11 Dec 2021 10:54:37: 18000000 INFO @ Sat, 11 Dec 2021 10:54:37: 21000000 INFO @ Sat, 11 Dec 2021 10:54:37: #1 tag size is determined as 58 bps INFO @ Sat, 11 Dec 2021 10:54:37: #1 tag size = 58 INFO @ Sat, 11 Dec 2021 10:54:37: #1 total tags in treatment: 21050802 INFO @ Sat, 11 Dec 2021 10:54:37: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:54:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:54:38: #1 tags after filtering in treatment: 21050802 INFO @ Sat, 11 Dec 2021 10:54:38: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:54:38: #1 finished! INFO @ Sat, 11 Dec 2021 10:54:38: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:54:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:54:39: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 10:54:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:54:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986102/SRX9986102.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986102/SRX9986102.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986102/SRX9986102.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986102/SRX9986102.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 10:54:42: 16000000 INFO @ Sat, 11 Dec 2021 10:54:47: 19000000 INFO @ Sat, 11 Dec 2021 10:54:53: 17000000 INFO @ Sat, 11 Dec 2021 10:54:57: 20000000 INFO @ Sat, 11 Dec 2021 10:55:03: 18000000 INFO @ Sat, 11 Dec 2021 10:55:07: 21000000 INFO @ Sat, 11 Dec 2021 10:55:08: #1 tag size is determined as 58 bps INFO @ Sat, 11 Dec 2021 10:55:08: #1 tag size = 58 INFO @ Sat, 11 Dec 2021 10:55:08: #1 total tags in treatment: 21050802 INFO @ Sat, 11 Dec 2021 10:55:08: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:55:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:55:08: #1 tags after filtering in treatment: 21050802 INFO @ Sat, 11 Dec 2021 10:55:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:55:08: #1 finished! INFO @ Sat, 11 Dec 2021 10:55:08: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:55:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:55:10: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 10:55:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:55:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986102/SRX9986102.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986102/SRX9986102.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986102/SRX9986102.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986102/SRX9986102.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 10:55:13: 19000000 INFO @ Sat, 11 Dec 2021 10:55:23: 20000000 INFO @ Sat, 11 Dec 2021 10:55:33: 21000000 INFO @ Sat, 11 Dec 2021 10:55:33: #1 tag size is determined as 58 bps INFO @ Sat, 11 Dec 2021 10:55:33: #1 tag size = 58 INFO @ Sat, 11 Dec 2021 10:55:33: #1 total tags in treatment: 21050802 INFO @ Sat, 11 Dec 2021 10:55:33: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:55:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:55:34: #1 tags after filtering in treatment: 21050802 INFO @ Sat, 11 Dec 2021 10:55:34: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:55:34: #1 finished! INFO @ Sat, 11 Dec 2021 10:55:34: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:55:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:55:35: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 10:55:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:55:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986102/SRX9986102.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986102/SRX9986102.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986102/SRX9986102.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986102/SRX9986102.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。