Job ID = 14168780 SRX = SRX9910773 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 61764665 spots for SRR13498844/SRR13498844.sra Written 61764665 spots for SRR13498844/SRR13498844.sra fastq に変換しました。 bowtie でマッピング中... Your job 14169863 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:27:30 61764665 reads; of these: 61764665 (100.00%) were unpaired; of these: 4488715 (7.27%) aligned 0 times 39609493 (64.13%) aligned exactly 1 time 17666457 (28.60%) aligned >1 times 92.73% overall alignment rate Time searching: 00:27:30 Overall time: 00:27:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 11527339 / 57275950 = 0.2013 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 20:55:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9910773/SRX9910773.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9910773/SRX9910773.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9910773/SRX9910773.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9910773/SRX9910773.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 20:55:16: #1 read tag files... INFO @ Fri, 10 Dec 2021 20:55:16: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 20:55:23: 1000000 INFO @ Fri, 10 Dec 2021 20:55:30: 2000000 INFO @ Fri, 10 Dec 2021 20:55:37: 3000000 INFO @ Fri, 10 Dec 2021 20:55:43: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 20:55:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9910773/SRX9910773.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9910773/SRX9910773.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9910773/SRX9910773.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9910773/SRX9910773.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 20:55:46: #1 read tag files... INFO @ Fri, 10 Dec 2021 20:55:46: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 20:55:51: 5000000 INFO @ Fri, 10 Dec 2021 20:55:53: 1000000 INFO @ Fri, 10 Dec 2021 20:55:58: 6000000 INFO @ Fri, 10 Dec 2021 20:56:00: 2000000 INFO @ Fri, 10 Dec 2021 20:56:06: 7000000 INFO @ Fri, 10 Dec 2021 20:56:08: 3000000 INFO @ Fri, 10 Dec 2021 20:56:13: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 20:56:15: 4000000 INFO @ Fri, 10 Dec 2021 20:56:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9910773/SRX9910773.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9910773/SRX9910773.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9910773/SRX9910773.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9910773/SRX9910773.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 20:56:16: #1 read tag files... INFO @ Fri, 10 Dec 2021 20:56:16: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 20:56:21: 9000000 INFO @ Fri, 10 Dec 2021 20:56:22: 5000000 INFO @ Fri, 10 Dec 2021 20:56:23: 1000000 INFO @ Fri, 10 Dec 2021 20:56:28: 10000000 INFO @ Fri, 10 Dec 2021 20:56:29: 6000000 INFO @ Fri, 10 Dec 2021 20:56:31: 2000000 INFO @ Fri, 10 Dec 2021 20:56:36: 11000000 INFO @ Fri, 10 Dec 2021 20:56:36: 7000000 INFO @ Fri, 10 Dec 2021 20:56:38: 3000000 INFO @ Fri, 10 Dec 2021 20:56:43: 12000000 INFO @ Fri, 10 Dec 2021 20:56:44: 8000000 INFO @ Fri, 10 Dec 2021 20:56:45: 4000000 INFO @ Fri, 10 Dec 2021 20:56:51: 9000000 INFO @ Fri, 10 Dec 2021 20:56:51: 13000000 INFO @ Fri, 10 Dec 2021 20:56:52: 5000000 INFO @ Fri, 10 Dec 2021 20:56:58: 10000000 INFO @ Fri, 10 Dec 2021 20:56:58: 14000000 INFO @ Fri, 10 Dec 2021 20:57:00: 6000000 INFO @ Fri, 10 Dec 2021 20:57:05: 11000000 INFO @ Fri, 10 Dec 2021 20:57:06: 15000000 INFO @ Fri, 10 Dec 2021 20:57:07: 7000000 INFO @ Fri, 10 Dec 2021 20:57:13: 12000000 INFO @ Fri, 10 Dec 2021 20:57:13: 16000000 INFO @ Fri, 10 Dec 2021 20:57:14: 8000000 INFO @ Fri, 10 Dec 2021 20:57:20: 13000000 INFO @ Fri, 10 Dec 2021 20:57:21: 17000000 INFO @ Fri, 10 Dec 2021 20:57:22: 9000000 INFO @ Fri, 10 Dec 2021 20:57:27: 14000000 INFO @ Fri, 10 Dec 2021 20:57:28: 18000000 INFO @ Fri, 10 Dec 2021 20:57:29: 10000000 INFO @ Fri, 10 Dec 2021 20:57:34: 15000000 INFO @ Fri, 10 Dec 2021 20:57:36: 19000000 INFO @ Fri, 10 Dec 2021 20:57:36: 11000000 INFO @ Fri, 10 Dec 2021 20:57:42: 16000000 INFO @ Fri, 10 Dec 2021 20:57:43: 20000000 INFO @ Fri, 10 Dec 2021 20:57:43: 12000000 INFO @ Fri, 10 Dec 2021 20:57:49: 17000000 INFO @ Fri, 10 Dec 2021 20:57:51: 13000000 INFO @ Fri, 10 Dec 2021 20:57:51: 21000000 INFO @ Fri, 10 Dec 2021 20:57:56: 18000000 INFO @ Fri, 10 Dec 2021 20:57:58: 14000000 INFO @ Fri, 10 Dec 2021 20:57:58: 22000000 INFO @ Fri, 10 Dec 2021 20:58:03: 19000000 INFO @ Fri, 10 Dec 2021 20:58:05: 15000000 INFO @ Fri, 10 Dec 2021 20:58:06: 23000000 INFO @ Fri, 10 Dec 2021 20:58:10: 20000000 INFO @ Fri, 10 Dec 2021 20:58:12: 16000000 INFO @ Fri, 10 Dec 2021 20:58:13: 24000000 INFO @ Fri, 10 Dec 2021 20:58:18: 21000000 INFO @ Fri, 10 Dec 2021 20:58:20: 17000000 INFO @ Fri, 10 Dec 2021 20:58:21: 25000000 INFO @ Fri, 10 Dec 2021 20:58:25: 22000000 INFO @ Fri, 10 Dec 2021 20:58:27: 18000000 INFO @ Fri, 10 Dec 2021 20:58:28: 26000000 INFO @ Fri, 10 Dec 2021 20:58:32: 23000000 INFO @ Fri, 10 Dec 2021 20:58:34: 19000000 INFO @ Fri, 10 Dec 2021 20:58:36: 27000000 INFO @ Fri, 10 Dec 2021 20:58:39: 24000000 INFO @ Fri, 10 Dec 2021 20:58:42: 20000000 INFO @ Fri, 10 Dec 2021 20:58:43: 28000000 INFO @ Fri, 10 Dec 2021 20:58:46: 25000000 INFO @ Fri, 10 Dec 2021 20:58:49: 21000000 INFO @ Fri, 10 Dec 2021 20:58:51: 29000000 INFO @ Fri, 10 Dec 2021 20:58:54: 26000000 INFO @ Fri, 10 Dec 2021 20:58:56: 22000000 INFO @ Fri, 10 Dec 2021 20:58:58: 30000000 INFO @ Fri, 10 Dec 2021 20:59:01: 27000000 INFO @ Fri, 10 Dec 2021 20:59:03: 23000000 INFO @ Fri, 10 Dec 2021 20:59:05: 31000000 INFO @ Fri, 10 Dec 2021 20:59:08: 28000000 INFO @ Fri, 10 Dec 2021 20:59:11: 24000000 INFO @ Fri, 10 Dec 2021 20:59:13: 32000000 INFO @ Fri, 10 Dec 2021 20:59:15: 29000000 INFO @ Fri, 10 Dec 2021 20:59:18: 25000000 INFO @ Fri, 10 Dec 2021 20:59:20: 33000000 INFO @ Fri, 10 Dec 2021 20:59:23: 30000000 INFO @ Fri, 10 Dec 2021 20:59:25: 26000000 INFO @ Fri, 10 Dec 2021 20:59:28: 34000000 INFO @ Fri, 10 Dec 2021 20:59:30: 31000000 INFO @ Fri, 10 Dec 2021 20:59:32: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 20:59:35: 35000000 INFO @ Fri, 10 Dec 2021 20:59:38: 32000000 INFO @ Fri, 10 Dec 2021 20:59:39: 28000000 INFO @ Fri, 10 Dec 2021 20:59:43: 36000000 INFO @ Fri, 10 Dec 2021 20:59:45: 33000000 INFO @ Fri, 10 Dec 2021 20:59:47: 29000000 INFO @ Fri, 10 Dec 2021 20:59:50: 37000000 INFO @ Fri, 10 Dec 2021 20:59:53: 34000000 INFO @ Fri, 10 Dec 2021 20:59:54: 30000000 INFO @ Fri, 10 Dec 2021 20:59:58: 38000000 INFO @ Fri, 10 Dec 2021 21:00:01: 35000000 INFO @ Fri, 10 Dec 2021 21:00:01: 31000000 INFO @ Fri, 10 Dec 2021 21:00:05: 39000000 INFO @ Fri, 10 Dec 2021 21:00:08: 32000000 INFO @ Fri, 10 Dec 2021 21:00:08: 36000000 INFO @ Fri, 10 Dec 2021 21:00:12: 40000000 INFO @ Fri, 10 Dec 2021 21:00:15: 33000000 INFO @ Fri, 10 Dec 2021 21:00:16: 37000000 INFO @ Fri, 10 Dec 2021 21:00:19: 41000000 INFO @ Fri, 10 Dec 2021 21:00:23: 34000000 INFO @ Fri, 10 Dec 2021 21:00:23: 38000000 INFO @ Fri, 10 Dec 2021 21:00:27: 42000000 INFO @ Fri, 10 Dec 2021 21:00:30: 35000000 INFO @ Fri, 10 Dec 2021 21:00:31: 39000000 INFO @ Fri, 10 Dec 2021 21:00:34: 43000000 INFO @ Fri, 10 Dec 2021 21:00:37: 36000000 INFO @ Fri, 10 Dec 2021 21:00:38: 40000000 INFO @ Fri, 10 Dec 2021 21:00:42: 44000000 INFO @ Fri, 10 Dec 2021 21:00:45: 37000000 INFO @ Fri, 10 Dec 2021 21:00:46: 41000000 INFO @ Fri, 10 Dec 2021 21:00:49: 45000000 INFO @ Fri, 10 Dec 2021 21:00:52: 38000000 INFO @ Fri, 10 Dec 2021 21:00:53: 42000000 INFO @ Fri, 10 Dec 2021 21:00:55: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 21:00:55: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 21:00:55: #1 total tags in treatment: 45748611 INFO @ Fri, 10 Dec 2021 21:00:55: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 21:00:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 21:00:56: #1 tags after filtering in treatment: 45748611 INFO @ Fri, 10 Dec 2021 21:00:56: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 21:00:56: #1 finished! INFO @ Fri, 10 Dec 2021 21:00:56: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 21:00:56: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 21:00:58: #2 number of paired peaks: 15 WARNING @ Fri, 10 Dec 2021 21:00:58: Too few paired peaks (15) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 21:00:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9910773/SRX9910773.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910773/SRX9910773.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910773/SRX9910773.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910773/SRX9910773.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 21:00:59: 39000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 21:01:01: 43000000 INFO @ Fri, 10 Dec 2021 21:01:06: 40000000 INFO @ Fri, 10 Dec 2021 21:01:08: 44000000 INFO @ Fri, 10 Dec 2021 21:01:13: 41000000 INFO @ Fri, 10 Dec 2021 21:01:15: 45000000 INFO @ Fri, 10 Dec 2021 21:01:21: 42000000 INFO @ Fri, 10 Dec 2021 21:01:21: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 21:01:21: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 21:01:21: #1 total tags in treatment: 45748611 INFO @ Fri, 10 Dec 2021 21:01:21: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 21:01:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 21:01:22: #1 tags after filtering in treatment: 45748611 INFO @ Fri, 10 Dec 2021 21:01:22: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 21:01:22: #1 finished! INFO @ Fri, 10 Dec 2021 21:01:22: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 21:01:22: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 21:01:25: #2 number of paired peaks: 15 WARNING @ Fri, 10 Dec 2021 21:01:25: Too few paired peaks (15) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 21:01:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9910773/SRX9910773.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910773/SRX9910773.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910773/SRX9910773.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910773/SRX9910773.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 21:01:27: 43000000 INFO @ Fri, 10 Dec 2021 21:01:34: 44000000 INFO @ Fri, 10 Dec 2021 21:01:41: 45000000 INFO @ Fri, 10 Dec 2021 21:01:46: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 21:01:46: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 21:01:46: #1 total tags in treatment: 45748611 INFO @ Fri, 10 Dec 2021 21:01:46: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 21:01:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 21:01:47: #1 tags after filtering in treatment: 45748611 INFO @ Fri, 10 Dec 2021 21:01:47: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 21:01:47: #1 finished! INFO @ Fri, 10 Dec 2021 21:01:47: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 21:01:47: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 21:01:50: #2 number of paired peaks: 15 WARNING @ Fri, 10 Dec 2021 21:01:50: Too few paired peaks (15) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 21:01:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9910773/SRX9910773.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910773/SRX9910773.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910773/SRX9910773.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910773/SRX9910773.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling